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Molecular mechanisms of chromosomal rearrangement caused by topological changing of DNA.

Research Project

Project/Area Number 25291077
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Genetics/Chromosome dynamics
Research InstitutionNara Institute of Science and Technology

Principal Investigator

Maki Hisaji  奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20199649)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords染色体再編・維持 / 遺伝的不安定性 / 繰り返し配列
Outline of Final Research Achievements

Various kinds of repetitive sequence present in genomes of many species are known to be a cause of genetic instability which leads to genetic diseases and cancer. We found that a long inverted repeat is transformed to a cruciform DNA in a manner dependent on DNA replication. E. coli SbcCD appeared to have a novel enzymatic acitivity that cleaves such a cruciform DNA, and this enzyme perhaps causes the genetic instability at long inverted repeats.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (30 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 5 results,  Acknowledgement Compliant: 4 results) Presentation (23 results) (of which Invited: 4 results)

  • [Int'l Joint Research] University of Edinburgh(英国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Long inverted repeat transiently stalls DNA replication by forming hairpin structures on both leading and lagging strands2016

    • Author(s)
      Lai P.J., Lim C.T., Le H.P., Katayama T., Leach D.R.F, Furukohri A*., Maki H.
    • Journal Title

      Genes to Cells

      Volume: 21 Issue: 2 Pages: 136-145

    • DOI

      10.1111/gtc.12326

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A novel mode of nuclease action is revealed by the bacterial Mre11/Rad50 complex2015

    • Author(s)
      Lim C.T., Lai P.J., Leach D.R.F, Maki H., Furukohri A
    • Journal Title

      Nucleic Acids Research

      Volume: 43 Pages: 9804-9816

    • DOI

      10.1093/nar/gkv855

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Short CCG repeat in huntingtin gene is an obstacle for replicative DNA polymerases, potentially hampering progression of replication fork.2015

    • Author(s)
      Le HP, Masuda Y, Tsurimoto T, Maki S, Katayama T, Furukohri A, Maki H.
    • Journal Title

      Genes Cells.

      Volume: 10 Issue: 10 Pages: 817-833

    • DOI

      10.1111/gtc.12275

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Recombinase and translesion DNA polymerase decrease the speed of replication fork progression during the DNA damage response in Escherichia coli cells.2015

    • Author(s)
      Tan K.W., Pham M.T., Furukohri A, Maki H. and Akiyama T.M.
    • Journal Title

      Nucleic Acids Research

      Volume: 43 Issue: 3 Pages: 1715-1725

    • DOI

      10.1093/nar/gkv044

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] DNA polymeraes IV mediates efficient and quick recovery at N2-dG adducts.2014

    • Author(s)
      Ikeda M, Furukohri A, Philippin G, Loechler E, Akiyama MT, Katayama T, Fuchs RP, Maki H.
    • Journal Title

      Nucleic Acids Research

      Volume: 42 Issue: 13 Pages: 8461-8472

    • DOI

      10.1093/nar/gku547

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A single-molecule approach to DNA replication in Escherichia coli cells demonstrated that DNA polymerase III is a major determinant of fork speed.2013

    • Author(s)
      Pham M.T., Tan K.W., Sakumura Y., Okumura K., Maki H. and Akiyama T.M.
    • Journal Title

      Molecular Microbiology

      Volume: 90 Issue: 3 Pages: 584-596

    • DOI

      10.1111/mmi.12386

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 大腸菌のrecAまたはdinB遺伝子の過剰発現による複製フォーク進行の遅延2015

    • Author(s)
      Tan Kang Wei, Pham Minh Tuan, 古郡麻子, 真木壽治, 秋山昌広
    • Organizer
      日本分子生物学会第38回年会
    • Place of Presentation
      神戸ポートアイランド、兵庫県神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 大腸菌の栄養環境が酸化DNA損傷と活性酸素種に与える影響2015

    • Author(s)
      布瀬翔平, Tom Conrad, 安藤さやか, 豊本奈美江, 中村葵, 秋山昌広, 真木壽治
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      東北大学、宮城県仙台市
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 低pH培養環境は大腸菌細胞内の酸化DNA損傷レベルを上昇させる2015

    • Author(s)
      宮原亮太, 真木壽治
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      東北大学、宮城県仙台市
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Biochemical analysis reveals a novel action of bacterial Mre11/Rad50 complex2015

    • Author(s)
      古郡麻子, Lim Chew Theng, Lai Pey Jiun, David R.F. Leach, 真木壽治
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      東北大学、宮城県仙台市
    • Year and Date
      2015-09-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] recAまたはdinB遺伝子の過剰発現による複製フォーク速度の低下2015

    • Author(s)
      Tan Kang Wei, Pham Minh Tuan, 古郡麻子, 真木壽治, 秋山昌広
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      東北大学、宮城県仙台市
    • Year and Date
      2015-09-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] 大腸菌DNAポリメラーゼIの細胞内機能におけるβクランプの役割2015

    • Author(s)
      山本崇史, 真木壽治
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      東北大学、宮城県仙台市
    • Year and Date
      2015-09-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヌクレオチドプールとDNAの酸化損傷に起因する突然変異2015

    • Author(s)
      真木壽治
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      東北大学、宮城県仙台市
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 大腸菌のSOS応答による複製フォークの進行速度の低下2014

    • Author(s)
      Tang Kang Wei、Pham Minh Tuan、古郡麻子、奥村克純、真木寿治、秋山昌広
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜・神奈川県・横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Escherichia coli DNA polymerase IV mediates quick recpovery of repliaction forks stalled at N2-dG adducts.2014

    • Author(s)
      Furukohri, A., Ikead, M., Akiyama, M., Katayama, T., Fuchs R.P., and Maki, H.
    • Organizer
      The 9th 3R International Symposium
    • Place of Presentation
      御殿場高原ホテル・静岡県・御殿場市
    • Year and Date
      2014-11-11 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] A single molecule approach to DNA replication in Escherichia coli cells demonstrated that DNA polymerae III is a major determinant of fork speed.2014

    • Author(s)
      Pham, TM., Tan, K.W., Sakumura, Y., Okumura,K., Furukohri, A., Maki, H.,and Akiyama, T.M.
    • Organizer
      The 9th 3R International Symposium
    • Place of Presentation
      御殿場高原ホテル・静岡県・御殿場市
    • Year and Date
      2014-11-11 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大腸菌損傷乗り越え型 DNA Polymerase IV の複製フォークにおける役割2014

    • Author(s)
      古郡麻子、池田美央、西川義人、秋山昌広、片山 勉、Robert P. Fuchs、真木寿治
    • Organizer
      日本遺伝学会 第86回大会
    • Place of Presentation
      長浜バイオ大学・滋賀県・長浜市
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大腸菌のSOS応答による複製フォーク速度の減速2014

    • Author(s)
      Tang Kang Wei、Pham Minh Tuan、古郡麻子、奥村克純、真木寿治、秋山昌広
    • Organizer
      日本遺伝学会 第86回大会
    • Place of Presentation
      長浜バイオ大学・滋賀県・長浜市
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] A short CCG run in the Huntingtin gene is an obstacle for replicative DNA polymerases, potentially hampering fork progression.2014

    • Author(s)
      Hang, L.P., Furukohri, A., Masuda, Y., Maki, S., Katayama, T., Fuchs R.P., and Maki, H.
    • Organizer
      DNA polymerases: Biology, Diseases and Biomediacal Appliatiuon Conference 2014
    • Place of Presentation
      Robinson college, Cambridge (England)
    • Year and Date
      2014-08-31 – 2014-09-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Escherichia coli DNA polymerase IV mediates quick recpovery of repliaction forks stalled at N2-dG adducts.2014

    • Author(s)
      Furukohri, A., Ikead, M., Akiyama, M., Katayama, T., Fuchs R.P., and Maki, H.
    • Organizer
      DNA polymerases: Biology, Diseases and Biomediacal Appliatiuon Conference 2014
    • Place of Presentation
      Robinson college, Cambridge (England)
    • Year and Date
      2014-08-31 – 2014-09-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] A single molecule approach to DNA replication in Escherichia coli cells demonstrated that DNA polymerae III is a major determinant of fork speed.2014

    • Author(s)
      Pham, TM., Tan, K.W., Sakumura, Y., Okumura,K., Furukohri, A., Maki, H.,and Akiyama, T.M.
    • Organizer
      DNA polymerases: Biology, Diseases and Biomediacal Appliatiuon Conference 2014
    • Place of Presentation
      Robinson college, Cambridge (England)
    • Year and Date
      2014-08-31 – 2014-09-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大腸菌細胞におけるDNA複製フォークの進行速度に関する一分子解析.2013

    • Author(s)
      Pham Minh Tuan、Tang Kang Wei、作村諭一、奥村克純、真木寿治、秋山昌広
    • Organizer
      第36回 日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、神戸市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌細胞における複製フォークの進行速度に関する一分子解析.2013

    • Author(s)
      Pham Minh Tuan、Tang Kang Wei、作村諭一、奥村克純、真木寿治、秋山昌広
    • Organizer
      第22回 DNA複製・組換え・修復ワークショップ
    • Place of Presentation
      ホテルニュー水戸屋、仙台市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌 Pol IV の働きにより複製フォークはN2-dG DNA損傷を効率よく乗り越えて進行を続ける2013

    • Author(s)
      古郡麻子、池田美央、秋山昌広、片山 勉、Robert P. Fuchs、真木寿治
    • Organizer
      第22回 DNA複製・組換え・修復ワークショップ
    • Place of Presentation
      ホテルニュー水戸屋、仙台市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 自然突然変異とDNAポリメラーゼの分子機構研究2013

    • Author(s)
      真木寿治
    • Organizer
      日本遺伝学会 第85回大会
    • Place of Presentation
      慶応大学日吉キャンパス、横浜市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] A sequence flanking CAG/CTG trinucleotide repeats in the gene responsible for Hiungtington's disease has a potential for blocking DNA polymerases and may cause the replication fork arrest.2013

    • Author(s)
      Phuong Hang Le、古郡麻子、増田雄司、片山 勉、真木智子、真木寿治
    • Organizer
      日本遺伝学会 第85回大会
    • Place of Presentation
      慶応大学日吉キャンパス、横浜市
    • Related Report
      2013 Annual Research Report
  • [Presentation] DNA polymerase III is a major determinat of replication fork speed in Escherichia coli.2013

    • Author(s)
      Pham Minh Tuan、Tang Kang Wei、作村諭一、奥村克純、真木寿治、秋山昌広
    • Organizer
      日本遺伝学会 第85回大会
    • Place of Presentation
      慶応大学日吉キャンパス、横浜市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌細胞のDNA複製フォーク速度の単分子解析.2013

    • Author(s)
      Pham Minh Tuan、Tang Kang Wei、作村諭一、奥村克純、真木寿治、秋山昌広
    • Organizer
      日本遺伝学会 第85回大会
    • Place of Presentation
      慶応大学日吉キャンパス、横浜市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌細胞のDNA複製フォーク速度の単分子解析.2013

    • Author(s)
      Pham Minh Tuan、Tang Kang Wei、作村諭一、奥村克純、真木寿治、秋山昌広
    • Organizer
      第10回 21世紀大腸菌研究会
    • Place of Presentation
      ラフォーレ修善寺、伊豆市
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2022-01-31  

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