Molecular mechanisms of chromosomal rearrangement caused by topological changing of DNA.
Project/Area Number |
25291077
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Genetics/Chromosome dynamics
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
Maki Hisaji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20199649)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
|
Keywords | 染色体再編・維持 / 遺伝的不安定性 / 繰り返し配列 |
Outline of Final Research Achievements |
Various kinds of repetitive sequence present in genomes of many species are known to be a cause of genetic instability which leads to genetic diseases and cancer. We found that a long inverted repeat is transformed to a cruciform DNA in a manner dependent on DNA replication. E. coli SbcCD appeared to have a novel enzymatic acitivity that cleaves such a cruciform DNA, and this enzyme perhaps causes the genetic instability at long inverted repeats.
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Report
(4 results)
Research Products
(30 results)
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[Presentation] Escherichia coli DNA polymerase IV mediates quick recpovery of repliaction forks stalled at N2-dG adducts.2014
Author(s)
Furukohri, A., Ikead, M., Akiyama, M., Katayama, T., Fuchs R.P., and Maki, H.
Organizer
The 9th 3R International Symposium
Place of Presentation
御殿場高原ホテル・静岡県・御殿場市
Year and Date
2014-11-11 – 2014-11-21
Related Report
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[Presentation] A single molecule approach to DNA replication in Escherichia coli cells demonstrated that DNA polymerae III is a major determinant of fork speed.2014
Author(s)
Pham, TM., Tan, K.W., Sakumura, Y., Okumura,K., Furukohri, A., Maki, H.,and Akiyama, T.M.
Organizer
The 9th 3R International Symposium
Place of Presentation
御殿場高原ホテル・静岡県・御殿場市
Year and Date
2014-11-11 – 2014-11-21
Related Report
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[Presentation] A short CCG run in the Huntingtin gene is an obstacle for replicative DNA polymerases, potentially hampering fork progression.2014
Author(s)
Hang, L.P., Furukohri, A., Masuda, Y., Maki, S., Katayama, T., Fuchs R.P., and Maki, H.
Organizer
DNA polymerases: Biology, Diseases and Biomediacal Appliatiuon Conference 2014
Place of Presentation
Robinson college, Cambridge (England)
Year and Date
2014-08-31 – 2014-09-04
Related Report
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[Presentation] Escherichia coli DNA polymerase IV mediates quick recpovery of repliaction forks stalled at N2-dG adducts.2014
Author(s)
Furukohri, A., Ikead, M., Akiyama, M., Katayama, T., Fuchs R.P., and Maki, H.
Organizer
DNA polymerases: Biology, Diseases and Biomediacal Appliatiuon Conference 2014
Place of Presentation
Robinson college, Cambridge (England)
Year and Date
2014-08-31 – 2014-09-04
Related Report
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[Presentation] A single molecule approach to DNA replication in Escherichia coli cells demonstrated that DNA polymerae III is a major determinant of fork speed.2014
Author(s)
Pham, TM., Tan, K.W., Sakumura, Y., Okumura,K., Furukohri, A., Maki, H.,and Akiyama, T.M.
Organizer
DNA polymerases: Biology, Diseases and Biomediacal Appliatiuon Conference 2014
Place of Presentation
Robinson college, Cambridge (England)
Year and Date
2014-08-31 – 2014-09-04
Related Report
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