Evolution of laminarialean kelp beds deduced from the genomics and morphological features

• Project/Area Number: 25291087
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Kobe University
• Principal Investigator: Kawai Hiroshi 神戸大学, 内海域環境教育研究センター, 教授 (30161269)
• Co-Investigator(Kenkyū-buntansha): HANYUDA TAKEAKI 神戸大学, 自然科学系先端融合研究環内海域環境教育研究センター, 助教 (40379334) ; YAMAGISHI TAKAHIRO 神戸大学, 自然科学系先端融合研究環, 助教 (30379333) ; NAGASATO CHIKAKO 北海道大学, 北方生物圏フィールド科学センター, 准教授 (00374710) ; 本村 泰三 北海道大学, 学内共同利用施設等, 教授 (30183974)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: コンブ目 / 進化 / 分子系統 / トランスクリプトーム / 褐藻 / 網羅的ｃDNA塩基配列解析 / 生物地理 / 藻場構成種 / 網羅的cDNA塩基配列解析

Outline of Final Research Achievements

The molecular phylogeny of derived Laminariales using 6 chloroplast and 6 mitochondrial gene sequences revealed that Alariaceae and Agaraceae/Laminariaceae diverged from Aureophycaceae and the Agaraceae branched. This suggested that the formation of mucilaginous organs and pneumatocysts occurred in the relatively early stage of the evolution of derived Laminariales, and the adaptation to temperate habitat from cold-water habitat, and dispersal to the Southern Hemisphere occurred several time independently in different lineages in laminarialean families. Transcroptome analyses suggested that the diversification of mannurate c5 epimarase and metaroprotease played important roles in the anatomical differenciation of derived Laminariales.

Research Products

• [Journal Article] Phylogeographic analysis of the brown alga Cutleria multifida (Tilopteridales, Phaeophyceae) suggests a complicated introduction history (2016)
  • Author(s): Kawai H., Kogishi K., Hanyuda T., Arai S., Gurgel C.F., Nelson W., Meinesz A., Tsiamis K. and Peters A.F.
  • Journal Title: Phycological Research Volume: 64 Issue: 1 Pages: 3-10
  • DOI: 10.1111/pre.12113
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Taxonomic revision of Papenfussiella species (Chordariaceae, Phaeophyceae) in the Northern Hemisphere (2016)
  • Author(s): Kawai, H., Miyoshi, K., Hanyuda, T.
  • Journal Title: Phycologia Volume: 55 Issue: 3 Pages: 308-317
  • DOI: 10.2216/15-139.1
  • Peer Reviewed
• [Journal Article] Flagellar waveforms of gametes in the brown alga Ectocarpus siliculosus. (2016)
  • Author(s): Kinoshita N, Shiba K, Inaba K, Fu G, Nagasato C and Motomura T.
  • Journal Title: European Journal of Phycology Volume: 51 Issue: 2 Pages: 139-148
  • DOI: 10.1080/09670262.2015.1109144
  • NAID: 120006220013
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] An introduced population of Chorda asiatica (Chordaceae, Laminariales) in Puget Sound, North America (2015)
  • Author(s): Kawai H., Hanyuda T., Mumford T. and Waaland J.R.
  • Journal Title: Phycological Research Volume: 63 Issue: 2 Pages: 154-158
  • DOI: 10.1111/pre.12089
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Taxonomy, morphology and genetic variation of Nitella flexilis var. bifurcata (Charales, Characeae) from Japan (2015)
  • Author(s): Sakayama, H., Kai, A, Nishiyama, M., Watanabe, M. M., Kato, S., Ito, M., Nozaki, H. and Kawai, H.
  • Journal Title: Phycological Research Volume: 63 Issue: 3 Pages: 159-166
  • DOI: 10.1111/pre.12085
  • Peer Reviewed
• [Journal Article] Taxonomic re-examination of Japanese Halimeda species using genetic markers, and proposal of a new species Halimeda ryukyuensis (Bryopsidales, Chlorophyta) (2015)
  • Author(s): Kojima R., Hanyuda T. and Kawai H.
  • Journal Title: Phycological Research Volume: 63 Issue: 3 Pages: 178-188
  • DOI: 10.1111/pre.12095
  • Peer Reviewed
• [Journal Article] Diversity of Halimeda (Chlorophyta) from the Thai–Malay Peninsula (2015)
  • Author(s): Pongparadon S., Zuccarello G., Phang S.-M., Kawai H., Hanyuda T. and Prathep A.
  • Journal Title: Phycologia Volume: 54 Issue: 4 Pages: 349-366
  • DOI: 10.2216/14-108.1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Reproductive morphology and DNA sequences of the brown alga Platysiphon verticillatus support the new combination Platysiphon glacialis (2015)
  • Author(s): Kawai H., Hanyuda T., Yamagishi T., Kai A., Lane C.E., McDevit D., Küpper F.C. and Saunders G.W.
  • Journal Title: Journal of Phycology Volume: 51 Issue: 5 Pages: 910-917
  • DOI: 10.1111/jpy.12331
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular phylogeny of two unusual brown algae, Phaeostrophion irregulare and Platysiphon glacialis, proposal of the Stschapoviales ord. nov. and Platysiphonaceae fam. nov., and a re-examination of divergence times for brown algal orders (2015)
  • Author(s): Kawai H., Hanyuda T., Draisma G.A. Wilce R.T. and Andersen R.A.
  • Journal Title: Journal of Phycology Volume: 51 Issue: 5 Pages: 918-928
  • DOI: 10.1111/jpy.12332
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Aribbon-like structure in the ejective organelle of green microalga Pyramimonas parkeae (Prasinophyceae) consists of core histones and polymers containing N-acetyl-glucosamine. (2015)
  • Author(s): Yamagishi T. and Kawai H.
  • Journal Title: Protist Volume: 166 Issue: 5 Pages: 522-533
  • DOI: 10.1016/j.protis.2015.08.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ubiquitous distribution of helmchrome in phototactic swarmers of the stramenopiles. (2015)
  • Author(s): Fu G., Nagasato C., Yamagishi T., Kawai H., Okuda K., Yoshitake T., Horiguchi T., Motomura T.
  • Journal Title: Protplasma Volume: 253 Issue: 3 Pages: 929-941
  • DOI: 10.1007/s00709-015-0857-7
  • Peer Reviewed
• [Journal Article] Plasmodesmata of brown algae. (2015)
  • Author(s): Terauchi, M, Nagasato, C, and Motomura, T.
  • Journal Title: J. Plant Res. Volume: 128 Issue: 1 Pages: 7-15
  • DOI: 10.1007/s10265-014-0677-4
  • Peer Reviewed
• [Journal Article] Development and function of plasmodesmata in Fucus zygotes (2015)
  • Author(s): Nagasato, C., Tanaka, A. and Motomura, T
  • Journal Title: Bot. Mar. Volume: 58 Pages: 229-238
  • Peer Reviewed
• [Journal Article] Phylogeographic data revealed shallow genetic structure in the kelp Saccharina japonica (Laminariales, Phaeophyta). (2015)
  • Author(s): Zhang J, Yao JT, Sun ZM, Fu G, Galanin DA, Nagasato C, Motomura T, Hu ZM and Duan DL.
  • Journal Title: BMC Evolutionary Biology Volume: 15 Issue: 1 Pages: 1-12
  • DOI: 10.1186/s12862-015-0517-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spermatogenesis and auxospore structure in the multipolar centric diatom Hydrosera (2015)
  • Author(s): Masahiko Idei, Shinya Sato, Chikako Nagasato, Taizo Motomura, Kensuke Toyoda, Tamotsu Nagumo, David G. Mann
  • Journal Title: Journal of Phycology Volume: 51 Issue: 1 Pages: 144-158
  • DOI: 10.1111/jpy.12261
  • Peer Reviewed
• [Journal Article] Occurrence of an endangered species Nitellopsis obtusa (Charales, Charophyceae) in western Japan and the genetic differences within and among Japanese populations (2015)
  • Author(s): Kato, S., Kawai, H., Takimoto, M., Suga, H., Yohda, K., Horiya, K., Higuchi, S., Sakayama, H. 2015. Occurrence of an endangered species Nitellopsis obtusa (Charales, Charophyceae) in western Japan and the genetic differences within and among Japanese populations. Phycol. Res.
  • Journal Title: Phycol. Res. Volume: 63 Pages: 00-00
  • NAID: 40020140316
  • Peer Reviewed
• [Journal Article] Taxonomy, morphology and genetic variation of Nitella flexilis var. bifurcata (Charales, Characeae) from Japan (2015)
  • Author(s): Sakayama, H., Kai, A., Nishiyama, M., Watanabe, M.M., Kato, S., Ito, M., Nozaki, H., Kawai, H.
  • Journal Title: Phycol. Res. Volume: 63 Pages: 00-00
  • Peer Reviewed
• [Journal Article] Taxonomic re-examination of Japanese Halimeda species using genetic markers, and proposal of a new species Halimeda ryukyuensis (Bryopsidales, Chlorophyta) (2015)
  • Author(s): Kojima, R., Hanyuda, T., Kawai, H.
  • Journal Title: Phycol. Res. Volume: 63 Pages: 00-00
  • Peer Reviewed
• [Journal Article] Ligulate Desmarestia (Desmarestiales, Phaeophyceae) revised: D. japonica sp. nov. and D. dudresnayi differ from D. ligulata. (2014)
  • Author(s): Yang, E.C., Peters, A.F., Kawai, H., Stern, R., Hanyuda, T., Bárbara, T., Müller, D.G., Srittmatter, M. Prud’Homme van Reine, W.F., Küpper, F.C.
  • Journal Title: J. Phycol. Volume: 50 Issue: 1 Pages: 149-166
  • DOI: 10.1111/jpy.12148
  • Peer Reviewed
• [Journal Article] Radioactive cesium accumulation in seaweeds by the Fukushima 1 Nuclear Power Plant accident – two years’ monitoring at Iwaki and its vicinity. (2014)
  • Author(s): Kawai, H., Kitamura, A., Mimura, M., Mimura, T., Tahara, T., Aida, D., Sato, K., H. Sasaki.
  • Journal Title: J. Plant Res. Volume: 127 Issue: 1 Pages: 23-42
  • DOI: 10.1007/s10265-013-0603-1
  • Peer Reviewed / Open Access
• [Journal Article] Four newly recorded species of the calcified marine brown macroalgal genus Padina (Dictyotales, Phaeophyceae) for Australia (2014)
  • Author(s): Ni-Ni-Win, Zhong-Min Sun, Z.-M., Hanyuda, T., Kurihara, A., Gurgel, C.F.D., Kawai, H.
  • Journal Title: Aust. Syst. Bot. Volume: 26 Issue: 6 Pages: 448-465
  • DOI: 10.1071/sb13025
  • Peer Reviewed
• [Journal Article] Recent advances in the phylogeny and taxonomy of Laminariales (2014)
  • Author(s): Kawai, H.
  • Journal Title: Perspectives in Phycology Volume: 1 Pages: 27-40
  • Peer Reviewed / Open Access
• [Journal Article] Comparative transcriptome analysis of Discosporangium mesarthrocarpum (Phaeophyceae), Schizocladia ischiensis (Schizocladiophyceae) and Phaeothamnion confervicola (Phaeothamniophyceae), with special reference to cell wall-related genes (2014)
  • Author(s): Yamagishi, T., Müller, D.G., Kawai, H.
  • Journal Title: J. Phycol. Volume: 50 Pages: 543-551
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A pilot-scale floating closed culture system for the multicellular cyanobacterium Arthrospira platensis NIES-03 (2014)
  • Author(s): Toyoshima, M., Yamagishi, T., Aikawa, S., Kondo, A., Kawai, H.
  • Journal Title: J. Appl. Phycol. Volume: 27 Issue: 6 Pages: 0-0
  • DOI: 10.1007/s10811-014-0484-2
  • Peer Reviewed / Open Access
• [Journal Article] Proteomics analysis of heterogeneous flagella in brown alga (Stramenopiles) (2014)
  • Author(s): Fu, G., Nagasato, C., Oka, S., Cock, M. J. and Motomura, T.
  • Journal Title: Protist Volume: 165 Issue: 5 Pages: 662-675
  • DOI: 10.1016/j.protis.2014.07.007
  • NAID: 120005906760
  • Peer Reviewed
• [Journal Article] Identification of three proteins involved in fertilization and parthenogenetic development of a brown alga, Scytosiphon lomentaria (2014)
  • Author(s): Jong Won Han, J. W., Tatyana A. Klochkova, T. A, Shim, J., Nagasato, C. Motomura, T., and Kim, G. H.
  • Journal Title: Planta Volume: 240 Issue: 6 Pages: 1253-1267
  • DOI: 10.1007/s00425-014-2148-5
  • Peer Reviewed
• [Journal Article] Mesospora elongata sp. nov. (Ralfsiales, Phaeophyceae), a new crustose brown algal species from the Indo-Pacific region (2013)
  • Author(s): Poong, S.-W., Lim P.-E., Phang, S.-M., Gerung G., Kawai, H.
  • Journal Title: Phycologia Volume: 52 Issue: 1 Pages: 84-81
  • DOI: 10.2216/12-42.1
  • Peer Reviewed
• [Journal Article] A molecular-assisted floristic survey of crustose brown algae (Phaeophyceae) from Malaysia and Lombok Island, Indonesia based on rbcL and partial cox1 genes (2013)
  • Author(s): Poong, S.-W., Lim P.-E., Phang, S.-M., Sunarpi, H., West, J.A., Kawai, H.
  • Journal Title: J. Appl. Phycol. Volume: 26 Issue: 2 Pages: 1231-1242
  • DOI: 10.1007/s10811-013-0081-9
  • Peer Reviewed
• [Journal Article] Direct conversion of Spirulina to ethanol without pretreatment of enzymatic hydrolysis processes (2013)
  • Author(s): Aikawa, S., Joseph, A., Yamada, R., Izumi, Y., Yamagishi, T., Matsuda, F., Kawai, H., Chang, J-S., Hasunuma, T., Kondo, A.
  • Journal Title: Energy Environ. Sci. Volume: 6 Pages: 1844-1849
  • Peer Reviewed
• [Journal Article] Ancestral reproductive structure in basal kelp Aureophycus aleuticus (2013)
  • Author(s): Kawai, H., Hanyuda, T., Ridgway, L.M., Holser, K.
  • Journal Title: Sci. Rep. Volume: 3 Issue: 1 Pages: 2491-2491
  • DOI: 10.1038/srep02491
  • NAID: 120005356886
  • Peer Reviewed
• [Presentation] Marine algae carried across the North Pacific on Japanese Tsunami Marine Debris (JTMD) and their invation threat to the coasts of Oregon and Washington, USA (2016)
  • Author(s): Hansen, G., Hanyuda, T., Kawai, H.
  • Organizer: 9th International Conference on Marine Bioinvasions
  • Place of Presentation: The Menzies Sydney Hotel, Sydney
  • Year and Date: 2016-01-19
  • Int'l Joint Research
• [Presentation] Taxonomic revision of Papenfussiella species in the Northern Hemispher (2015)
  • Author(s): Kawai, H., Miyoshi, K., Hanyuda, T.
  • Organizer: European Phycological Congress
  • Place of Presentation: Novotel, London
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] 北米産ツルモ属の系統地理–新たに確認された越境移入– (2015)
  • Author(s): 川井浩史・羽生田岳昭・T. Mumford・J.R. Waaland
  • Organizer: 日本藻類学会第39回大会
  • Place of Presentation: 九州大学
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] プラシノ藻Pyramimonas parkeaeにおけるエジェクトソーム様構造の分子同定：ヒストンと酸性多糖の重合体から成る新奇ポリマー (2015)
  • Author(s): 山岸隆博・川井浩史
  • Organizer: 日本藻類学会第39回大会
  • Place of Presentation: 九州大学
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Genomic study of the brown algal model organism Ectocarpus (2014)
  • Author(s): Kawai, H.
  • Organizer: Marchantia Workshop 2014
  • Place of Presentation: Centennial Hall, Kobe University, Kobe
  • Year and Date: 2014-12-08 – 2014-12-10
  • Invited
• [Presentation] Molecular phylogeny of Cladosiphon species, and proposal of a new species from central Japan (2014)
  • Author(s): Kawai, H., Kim, S.H., Ichikawa, Y., Uwai, S. Hanyuda, T., Peters, A.F.
  • Organizer: 7th Asian Pacific Phycological Forum
  • Place of Presentation: Wuhan Eastlake International Conference Center, Wuhan, China
  • Year and Date: 2014-09-20 – 2014-09-24
• [Presentation] 褐藻の細胞壁形成における金標識抗フコイダン抗体の分布 (2014)
  • Author(s): 関田論子・川井浩史・奥田一雄
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学生田キャンパス
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] Early evolution of Laminariales (2014)
  • Author(s): Kawai, H.
  • Organizer: 28th Congress of the Phycological Society of Southern Africa
  • Place of Presentation: Melkbosstrand, South Africa
• [Presentation] 系統保存株を用いた褐藻類の多遺伝子分子系統解析とPlatysiphon verticillatusの分類の再検討 (2014)
  • Author(s): 川井浩史・羽生田岳昭・R.J. Andersen・R. Wilce
  • Organizer: 日本藻類学会第38回大会
  • Place of Presentation: 東邦大学
• [Presentation] Life history, molecular phylogeny and taxonomic revision of Platysiphon verticillatus (Phaeophyceae) (2013)
  • Author(s): awai, H., Hanyuda, T., Kai, A., Yamagishi, T., Saunders, G.W., Lane, C., McDevit, D., Küpper, F.C., Wilce, R.T.
  • Organizer: 52nd Northeast Algal Society Annual Symposium
  • Place of Presentation: Mystic, Connecticut, USA
• [Presentation] Sorus formation of the basal system in Aureophycus aleuticus, and its taxonomic revision at familial rank (2013)
  • Author(s): Kawai, H., Hanyuda, T., Holser, K., Ridgeway, L.M.
  • Organizer: 10th International Phycological Congress
  • Place of Presentation: Orlando, Florida, USA
• [Presentation] Macroalgal culture collection in Kobe University and macroalgal genomics (2013)
  • Author(s): Kawai, H., Yamagishi, T., Kai, A., Yamaguchi, A., Hanyuda, T.
  • Organizer: 5th ANRRC International Meeting
  • Place of Presentation: Shonan-village Center, Hayama, Japan
  • Invited
• [Presentation] 褐藻類の多細胞化・大型化を細胞壁成分、有性生殖様式、生活史型の進化から考える (2013)
  • Author(s): 川井浩史・羽生田岳昭・山岸隆博
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学
• [Book] ‘Evolution and biogeography of laminarialean kelps’ (Ed. Z. Hu and C. Fraser) in ‘Seaweed Phylogeography’ (2016)
  • Author(s): Kawai, H., Hanyuda, T., Uwai, S.
  • Total Pages: 22
  • Publisher: Springer
• [Book] ‘Introduction (Heterokontobionta p.p.), Cryptophyta, Dinophyta, Haptophyta, Heterkontophyta (except diatoms, Phaeophyceae, Eustigmatophyceae), Chlorarachniophyta, Euglenophyta’ (Ed. W. Frey) in ‘Syllabus of Plant Families – A. Engler’s Syllabus der Pflanzenfamilien Part 2/1 Photoautotrophic eukaryotic Algae (2015)
  • Author(s): Nakayama, T., Kawai, H.
  • Total Pages: 30
  • Publisher: Borntraeger Verlagsbuchhandlung
• [Book] Syllabus of Plant Families Part 2/1 (2015)
  • Author(s): Nakayama, T., Kawai, H.
  • Total Pages: 324
  • Publisher: Borntraeger
• [Book] 光合成生物の進化と生命科学 (2014)
  • Author(s): 三村徹郎・川井浩史
  • Total Pages: 194
  • Publisher: 培風館