Studies on the evolutionary origin chromosomal differentiation and expansion process in a brachypterous grasshopper

• Project/Area Number: 25291088
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: University of the Ryukyus
• Principal Investigator: TATSUTA Haruki 琉球大学, 農学部, 教授 (50370268)
• Co-Investigator(Kenkyū-buntansha): 菅野 良一 北海道大学, (連合)農学研究科(研究院), 専門研究員 (00648826) ; 秋元 信一 北海道大学, (連合)農学研究科(研究院), 教授 (30175161)
• Research Collaborator: AKIMOTO Shin-ichi 北海道大学 ; BUGROV Alexander ロシア科学アカデミー, シベリア支部 ; WARCHALOWSKA-SLIWA Elzbieta ポーランド科学アカデミー ; GRZYWACZ Beata 日本学術振興会外国人, 特別研究員
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 種分化 / バッタ / 昆虫 / 遺伝学 / 分類学 / 進化 / 行動学 / フキバッタ / 染色体 / 生殖隔離 / マイクロサテライト / 遺伝構造 / 性選択 / 共生微生物 / 性的対立

Outline of Final Research Achievements

To explore spatial genetic structures using molecular markers in a brachypterous grasshopper Podisma sapporensis in which a new sex determination system has been established by the process of translocation between a autosome and X chromosome, we have designed primers that amplify the flanking regions of tandem repeats using the next generation sequencer. We also estimated approximate genome size in this species. Furthermore, we found the infection of endosymbiont in P. sapporensis. Copulatory behavior and copulation duration were also recorded and compared between different chromosomal races.

Research Products

• [Int'l Joint Research] ロシア科学アカデミーシベリア支部(ロシア連邦)
• [Int'l Joint Research] ポーランド科学アカデミー(ポーランド)
• [Int'l Joint Research] ロシア科学アカデミーシベリア支部(ロシア連邦)
• [Int'l Joint Research] ポーランド科学アカデミー(ポーランド)
• [Journal Article] First evidence of Wolbachia infection in populations of the grasshopper Podisma sapporensis Shiraki (Orthoptera, Acrididae) (2016)
  • Author(s): Bugrov, A. G., Y. Y. Ilinsky, A. A.Strunov, M. V. Zhukova, E. V. Kiseleva, S. Akimoto, and H. Tatsuta
  • Journal Title: Entomological Science Volume: 19 Issue: 3 Pages: 296-300
  • DOI: 10.1111/ens.12187
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Young giant water nymphs prefer larger prey: Changes in foraging behavior with nymphal growth in Kirkaldyia deyrolli (2016)
  • Author(s): Ohba, S. and H. Tatsuta
  • Journal Title: Biological Journal of the Linnean Society Volume: 117 Issue: 3 Pages: 601-606
  • DOI: 10.1111/bij.12693
  • Peer Reviewed / Open Access
• [Journal Article] Dimorphism of right-left asymmetry in the female genitalia of the brachypterous grasshopper Parapodisma mikado (Orthoptera: Acrididae): Asymmetric female genitalia (2016)
  • Author(s): Shimizu, D., Y. C. Sugano, K. Tsuchiya-Suzuki, G. Ito, and S. Akimoto
  • Journal Title: Entomological Science Volume: 19 Issue: 4 Pages: 452-457
  • DOI: 10.1111/ens.12211
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The first evidence of Wolbachia infection in populations of the grasshopper Podisma sapporensis Shiraki (Orthoptera, Acrididae) (2016)
  • Author(s): Bugrov, A.G., Y.Y. Ilinsky, A.A.Strunov, M.V.Zhukova, E.V.Kiseleva, S. Akimoto, and H. Tatsuta
  • Journal Title: Entomological Science Volume: in press
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] (Z)-13-Hexadecenyl acetate: a novel moth sex pheromone component from Herpetogramma submarginale (Lepidoptera: Crambidae). (2015)
  • Author(s): Yan Q, Kuriyama K, Nishikawa K, Tominaga S, Tatsuta H, Ando T, Naka H
  • Journal Title: Journal of Chemical Ecology Volume: 41 Issue: 5 Pages: 441-445
  • DOI: 10.1007/s10886-015-0576-8
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Free silver ion as the main cause of acute and chronic toxicity of silver nanoparticles to cladocerans (2015)
  • Author(s): Sakamoto, M., J-Y, Ha, S. Yonashima, C. Kataoka, H. Tatsuta, and S. Kashiwada
  • Journal Title: Archives of Environmental Contamination and Toxicology Volume: 68 Issue: 3 Pages: 500-509
  • DOI: 10.1007/s00244-014-0091-x
  • Peer Reviewed / Open Access
• [Journal Article] Female sex pheromones of two Japanese Saturniid species, Rhodinia fugax and Loepa sakaei: Identification, synthesis, and field evaluation (2015)
  • Author(s): Yan, Q., A. Kanegae, T. Miyachi, H. Naka, H. Tatsuta, and T. Ando
  • Journal Title: Journal of Chemical Ecology Volume: 41 Issue: 1 Pages: 1-8
  • DOI: 10.1007/s10886-014-0538-6
  • Peer Reviewed / Open Access
• [Journal Article] 遺伝的変異を考慮した感受性評価：ミジンコ集団を例に (2014)
  • Author(s): 立田晴記
  • Journal Title: 環境毒性学雑誌 Volume: 16「 Pages: 29-35
  • NAID: 130005089149
  • Peer Reviewed
• [Journal Article] Retrospective estimation of population-level effect of pollutants based on local adaptation and fitness cost of tolerance (2013)
  • Author(s): Tanaka, Y. and H. Tatsuta
  • Journal Title: Ecotoxicology Volume: 22 Issue: 5 Pages: 795-802
  • DOI: 10.1007/s10646-013-1081-x
  • Peer Reviewed
• [Presentation] Preliminary phylogeny of the tribe Podismini (Orthoptera: Acrididae: Melanoplinae) in Japan based on mitochondrial cytochrome c oxidase I gene sequences (2016)
  • Author(s): Grzywacz, B. and H. Tatsuta
  • Organizer: 12th International Congress of Orthopterology
  • Place of Presentation: Praia Do Sol Hotel, Ilheus, Brazil
  • Year and Date: 2016-10-30
  • Int'l Joint Research
• [Presentation] First evidence of Wolbachia infection in populations of grasshopper Podisma sapporensis (Orthoptera: Acrididae) (2016)
  • Author(s): Alexander Bugrov; Yury Ilinsky; Shin-ichi Akimoto & Haruki Tatsuta
  • Organizer: 12th International Congress of Orthopterology
  • Place of Presentation: Praia Do Sol Hotel, Ilheus, Brazil
  • Year and Date: 2016-10-30
  • Int'l Joint Research
• [Presentation] イモゾウムシの発音器官特定と発音時間に見られる地理的変異 (2016)
  • Author(s): 立田晴記，熊野了州，高梨琢磨
  • Organizer: 日本昆虫学会・日本応用動物昆虫学会合同大会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2016-03-26
• [Presentation] ゴマダラカミキリにおける振動に対する驚愕反応 (2016)
  • Author(s): 栗山賢人，立田晴記
  • Organizer: 日本昆虫学会・日本応用動物昆虫学会合同大会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2016-03-26
• [Presentation] イモゾウ虫のメス不妊虫は不妊虫放飼法に貢献するか？ (2016)
  • Author(s): 森本然，立田晴記，辻和希，熊野了州，鶴井香織
  • Organizer: 日本昆虫学会・日本応用動物昆虫学会合同大会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2016-03-26
• [Presentation] 天敵は「いる」だけで被食者に影響を与えるか－イリムサーキバラコマユバチの存在がイモゾウムシ次世代数に与える効果 (2015)
  • Author(s): 鶴井香織，熊野了州，原口大，立田晴記，豊里哲也，松山隆志
  • Organizer: 第59回日本応用動物昆虫学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] クワガタと環境研と私 (2015)
  • Author(s): 立田晴記
  • Organizer: 第59回日本応用動物昆虫学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] フタイロカミキリモドキにおける性的形質と配偶行動の個体群間変異 (2014)
  • Author(s): 里見大輔，小笠航，立田晴記，小汐千春，工藤慎一
  • Organizer: 日本動物行動学会第33回大会
  • Place of Presentation: 長崎大学
  • Year and Date: 2014-11-01 – 2014-11-03
• [Presentation] ミカドフキバッタの雌における交尾器の鏡像二型 (2014)
  • Author(s): 清水大輔・秋元信一
  • Organizer: 日本応用動物昆虫学会・日本昆虫学会共催 北海道支部大会
  • Place of Presentation: 北海道大学総合博物館
• [Book] Sexual differences in distress sounds in the West Indian sweet potato weevil Euscepes postfasciatus (2015)
  • Author(s): Tatsuta, H. and N. Kumano
  • Total Pages: 307
  • Publisher: World Scientific
• [Book] Morphometric analysis of intraspecific variation of the elytral shape between the wild- and mass-reared sweet potato weevil Cylas formicarius (2015)
  • Author(s): Kumano, N., K. Tsurui, T. Toyosato, and H. Tatsuta
  • Total Pages: 307
  • Publisher: World Scientific
• [Book] 行動生物学事典 (2013)
  • Author(s): 立田晴記
  • Total Pages: 637
  • Publisher: 東京化学同人
• [Book] Proceedings of the 2nd International Symposium of the Biological Shape Analysis (2013)
  • Author(s): Tatsuta, H., H. Iwata, and K. Goka
  • Total Pages: 215
  • Publisher: World Scientific
• [Remarks] 琉球大学農学部亜熱帯動物学講座
  • URL: http://w3.u-ryukyu.ac.jp/konken/
• [Patent(Industrial Property Rights)] 振動を用いた害虫の行動制御により植物を保護する方法 (2016)
  • Inventor(s): 高梨琢磨，小池卓二，松井康浩，上地奈美，立田晴記，他6名
  • Industrial Property Rights Holder: 高梨琢磨，小池卓二，松井康浩，上地奈美，立田晴記，他6名
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-244162
  • Filing Date: 2016-12-16