Response of the seagrass-bed ecosystem to global environmental changes with an emphasis on carbon sequestration and detrital food web

• Project/Area Number: 25291098
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Ecology/Environment
• Research Institution: The University of Tokyo
• Principal Investigator: Miyajima Toshihiro 東京大学, 大気海洋研究所, 助教 (20311631)
• Co-Investigator(Kenkyū-buntansha): 浜口 昌巳 国立研究開発法人水産研究・教育機構, 瀬戸内海区水産研究所, 干潟生産グループ長 (60371960) ; 堀 正和 国立研究開発法人水産研究・教育機構, 瀬戸内海区水産研究所, 主任研究員 (50443370)
• Research Collaborator: SATO Masaaki ; SHIMABUKURO Hiromori ; MORIMOTO Naoko ; HAMAOKA Akiko ; HAMAOKA Hideki
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
• Keywords: 海草藻場 / 食物網構造 / 炭素循環 / 海洋酸性化 / デトリタス / 底生動物 / 環境DNA / 微生物群集 / 生態系 / 海洋酸性化応答 / アマモ場 / 同位体標識実験 / 底生動物群集 / メソコズム / 微生物 / 無脊椎動物

Outline of Final Research Achievements

Response of the seagrass meadow ecosystem to ocean acidification induced by the rise of atmospheric CO2 was studied with an emphasis on the detrital food web. Major achievements can be summarized as follows. A novel eDNA technique was developed for tracing production, accumulation, and decomposition processes of seagrass-derived detritus. A clone library of seagrass rhizosphere bacterial community was established and analyzed to extract characteristics specific to such an environment. A novel 2H-based pulse-chase experimental method was developed to evaluate turnover time and conversion efficiency associated individual channels within the seagrass meadow epibenthic food web. Differential responses to ocean acidification between the grazing and detrital food chains of the seagrass meadow food web was demonstrated by mesocosm manipulation experiments with the aid of the developed pulse-chase technique.

Research Products

• [Journal Article] Geophysical constraints for organic carbon sequestration capacity of Zostera marina seagrass meadows and surrounding habitats (2017)
  • Author(s): Miyajima, T., Hori, M., Hamaguchi, M., Shimabukuro, H., Yoshida, G.
  • Journal Title: Limnology and Oceanography Volume: 62 Issue: 3 Pages: 954-972
  • DOI: 10.1002/lno.10478
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Geographic variability in organic carbon stock and accumulation rate in sediments of East and Southeast Asian seagrass meadows (2015)
  • Author(s): Miyajima T, Hori M, Hamaguchi M, Shimabukuro H, Adachi H, Yamano H, Nakaoka M
  • Journal Title: Global Biogeochemical Cycles Volume: 29 Issue: 4 Pages: 397-415
  • DOI: 10.1002/2014gb004979
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Quantitative DNA assays for detecting Zostera marina DNA in coastal Sediments (2017)
  • Author(s): Hori, M., Hamaguchi, M., Shimabukuro, H., Yoshida, G., Miyajima, T.
  • Organizer: JpGU-AGU Joint Meeting 2017
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2017-05-23
  • Int'l Joint Research
• [Presentation] Application of deuterium tracer techniques to food-web analysis of seagrass meadows (2017)
  • Author(s): Miyajima, T., Hori, M., Sato, M., Hamaoka, A., Hamaoka, H.
  • Organizer: JpGU-AGU Joint Meeting 2017
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2017-05-23
  • Int'l Joint Research
• [Presentation] Spatiotemporal variation in carbon and nitrogen stable isotope ratios of suspended and settling particles in coral reefs (2016)
  • Author(s): Miyajima, T., Morimoto, N., Tanaka, Y., Watanabe, A., Nakamura, T., Yamamoto, T., Nadaoka, K.
  • Organizer: JpGU-AGU Joint Meeting 2016
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2016-05-24
• [Presentation] 沿岸域における陸源物質動態:水、DIC、POM安定同位体比による評価 (2016)
  • Author(s): 森本直子、梅澤有、渡邉敦、Maria Lourdes San Diego-McGlone、Charissa M. Ferrera、Genevieve L. Regino、灘岡和夫、宮島利宏
  • Organizer: 日本地球惑星科学連合2016年大会
  • Place of Presentation: 幕張メッセ（千葉市）
  • Year and Date: 2016-05-24
• [Presentation] Millenary blue carbon extracted from temperate seagrass-bed sediments using DNA markers (2014)
  • Author(s): Masakazu Hori, Toshihiro Miyajima, Masami Hamaguchi
  • Organizer: 2nd Asian Marine Biology Symposium
  • Place of Presentation: 済州（韓国）
  • Year and Date: 2014-10-01 – 2014-10-04
• [Presentation] 熱帯・亜熱帯海草藻場堆積物における有機炭素の保存機構 ── 非吸着態有機炭素の重要性とその起源 (2014)
  • Author(s): 宮島利宏・堀正和・島袋寛盛・安達寛・浜口昌巳・仲岡雅裕・灘岡和夫
  • Organizer: 2014年日本地球惑星科学連合大会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-05-01
• [Presentation] DNAバイオマーカーを利用した海洋堆積物中有機炭素の起源評価の試み (2013)
  • Author(s): 浜口昌巳, 宮島利宏, 堀正和, 島袋寛盛, 吉田吾郎
  • Organizer: 2013年度日本地球化学会年会
  • Place of Presentation: 筑波大学（茨城県つくば市）
• [Presentation] Nutrient sources and export production in coral reefs: A case study on Shiraho coral reef (Southwest Japan)
  • Author(s): T. Miyajima
  • Organizer: International Workshop of Ocean acidification in Coral Reefs - Bridging gaps between field to laboratory studies
  • Place of Presentation: 東京大学理学部（東京都文京区）
• [Book] ブルーカーボン (2017)
  • Author(s): 堀正和・桑江朝比呂
  • Publisher: 地人書館