• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a method of molecular breeding involving epigenetic control of gene expression

Research Project

Project/Area Number 25292002
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionHokkaido University

Principal Investigator

KANAZAWA Akira  北海道大学, (連合)農学研究科(研究院), 准教授 (30281794)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords遺伝子発現 / 植物 / 分子育種 / 遺伝子 / 発現制御 / 色素 / RNA / 遺伝子発現制御 / エピジェネティクス / シトシンメチル化 / RNAサイレンシング / RNA機能
Outline of Final Research Achievements

This work was aimed to understand the factors affecting the efficient induction and stabilization of epigenetic changes from the view point of establishing modification of plant traits via epigenetic control of gene expression as a tool for molecular breeding. Epigenetic changes that occurred under a specific condition in plants that had RNA silencing were analyzed. The patterns of dynamic changes in small RNA production and cytosine methylation, together with the stability of the altered state, were uncovered.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (19 results)

All 2016 2015 2014 2013 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (12 results) (of which Invited: 3 results) Book (2 results)

  • [Journal Article] Deep sequencing uncovers commonality in small RNA profiles between transgene-induced and naturally occurring RNA silencing of chalcone synthase-A gene in petunia.2013

    • Author(s)
      Megumi Kasai
    • Journal Title

      BMC Genomics

      Volume: 14 Issue: 1 Pages: 63-63

    • DOI

      10.1186/1471-2164-14-63

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of RNA-directed DNA methylation of a transgene by simultaneously downregulating a ROS1 orthologue using a virus vector in Nicotiana benthamiana.Front2013

    • Author(s)
      Otagaki, S., Kasai, M., Masuta, C. and Kanazawa, A
    • Journal Title

      Genet

      Volume: 4 Pages: 44-44

    • DOI

      10.3389/fgene.2013.00044

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic variation in four maturity genes affects photoperiod insensitivity and PHYA-regulated post-flowering responses of soybean2013

    • Author(s)
      Xu M, Xu Z, Liu B, Kong F, Tsubokura Y, Watanabe S, Xia Z, Harada K, Kanazawa A, Yamada T and Abe J
    • Journal Title

      BMC Plant Biology

      Volume: 13 Issue: 1 Pages: 91-91

    • DOI

      10.1186/1471-2229-13-91

    • NAID

      120005316987

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of RNA-directed DNA methylation and heritable transcriptional gene silencing as a tool to engineer novel traits in plants2013

    • Author(s)
      Megumi Kasai
    • Journal Title

      Plant Biotechnology

      Volume: 30 Issue: 3 Pages: 233-241

    • DOI

      10.5511/plantbiotechnology.13.0319a

    • NAID

      10031202041

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epimutagenesis and its application for next generation breeding2013

    • Author(s)
      前川雅彦
    • Journal Title

      Breeding Research

      Volume: 15 Issue: 2 Pages: 42-50

    • DOI

      10.1270/jsbbr.15.42

    • NAID

      10031173700

    • ISSN
      1344-7629, 1348-1290
    • Related Report
      2013 Annual Research Report
  • [Presentation] ペチュニアにおけるカルコン合成酵素遺伝子のコサプレッションからのエピジェネティックな復帰:低分子RNA産生とシトシンメチル化状態の変化2016

    • Author(s)
      福本沙弥・河西めぐみ・種田晃人・金澤 章
    • Organizer
      日本育種学会第130回講演会
    • Place of Presentation
      鳥取大学(鳥取県、鳥取市)
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] レトロトランスポゾンの挿入を介した植物の環境適応とエピミュータジェネシス:重複遺伝子を持つダイズにおける事例2016

    • Author(s)
      金澤 章
    • Organizer
      2016年度国立遺伝学研究所 研究集会「転移因子と宿主の相互作用による生命機能と進化」
    • Place of Presentation
      国立遺伝学研究所(静岡県、三島市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ダイズにおけるレトロトランスポゾンSORE-1 のsmall RNA 産生とDNAメチル化2016

    • Author(s)
      御厨 駿
    • Organizer
      日本育種学会第129回講演会
    • Place of Presentation
      横浜市立大学(神奈川県、横浜市)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] ペチュニアのカルコン合成酵素遺伝子のコサプレッションとエピジェネティックな表現型の復帰過程におけるシトシンメチル化の動態2015

    • Author(s)
      福本沙弥
    • Organizer
      日本育種学会第128回講演会
    • Place of Presentation
      新潟大学(新潟県、新潟市)
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] ダイズにおけるRNAサイレンシングによる遺伝子発現制御とその利用2015

    • Author(s)
      金澤 章
    • Organizer
      第8回ダイズ研究会(招待講演)
    • Place of Presentation
      岩手大学(盛岡市)
    • Year and Date
      2015-03-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 遺伝子重複とレトロトランスポゾンの挿入を介したダイズの適応進化2014

    • Author(s)
      金澤 章
    • Organizer
      日本育種学会第56回シンポジウム(シンポジウム・ワークショップ)
    • Place of Presentation
      南九州大学(都城市)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 植物のRNAi機構

    • Author(s)
      金澤 章
    • Organizer
      2013年度国立遺伝学研究所 研究集会「転移因子と宿主の相互作用による生命機能」
    • Place of Presentation
      三島市 国立遺伝学研究所
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 開花時期の異なるダイズ系統間におけるイオンビーム照射による変異の比較

    • Author(s)
      土屋真弓
    • Organizer
      第8回高崎量子応用研究シンポジウム
    • Place of Presentation
      高崎市 高崎シティギャラリー
    • Related Report
      2013 Annual Research Report
  • [Presentation] ダイズにおけるトランスジーンのサイレンシングの広がりと伝達を制限する要素

    • Author(s)
      森 あゆみ
    • Organizer
      日本育種学会第124回講演会
    • Place of Presentation
      鹿児島市 鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 北海道の栽培ダイズ分化過程における遺伝子破壊を伴うレトロトランスポゾンSORE-1の転移

    • Author(s)
      土屋真弓
    • Organizer
      日本育種学会第124回講演会
    • Place of Presentation
      鹿児島市 鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] VIGSを用いたE1L遺伝子の機能解析

    • Author(s)
      徐 美蘭
    • Organizer
      日本育種学会第124回講演会
    • Place of Presentation
      鹿児島市 鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 組織学的解析から明らかになったダイズ植物体における外来遺伝子サイレンシングの領域拡張とその制限機構

    • Author(s)
      森 あゆみ
    • Organizer
      日本育種学会第125回講演会
    • Place of Presentation
      仙台市 東北大学
    • Related Report
      2013 Annual Research Report
  • [Book] Plant Gene Silencing: Methods and Protocols, Methods in Molecular Biology, vol. 12872015

    • Author(s)
      Akira Kanazawa, Megumi Kasai
    • Total Pages
      307
    • Publisher
      Springer Science + Business Media New York
    • Related Report
      2014 Annual Research Report
  • [Book] A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships2013

    • Author(s)
      Megumi Kasai
    • Total Pages
      613
    • Publisher
      InTech
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi