Molecular understanding of sequential pollination mechanism in plant reproduction.

• Project/Area Number: 25292005
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Mie University
• Principal Investigator: Suwabe Keita 三重大学, 生物資源学研究科, 准教授 (50451612)
• Co-Investigator(Kenkyū-buntansha): WATANABE Masao 東北大学, 大学院生命科学研究科, 教授 (90240522)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000); Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: 植物生殖 / 受粉 / 柱頭 / 乳頭細胞 / トランスクリプトーム / ライブイメージング / シロイヌナズナ / 花粉 / 自家不和合性 / バイオインフォマティックス / 突然変異体 / 細胞間情報認識 / 水輸送 / タイムラプス

Outline of Final Research Achievements

Pollination is an important early step in sexual plant reproduction. In angiosperms, it comprises a complex series of events, regulated by multiple processes, which must be completed effectively for successful seed production. Sequential pollination events, from pollen adhesion onto the stigma surface to pollen tube germination and elongation, occur on the stigma. In this study, we identified molecular factors in each pollination step, using LM-NGS transcriptome technology in stigmatic papilla cell. This finding will contribute a comprehensive molecular understanding of pollination mechanism in sexual plant reproduction.

Research Products

• [Journal Article] Comparative analysis of microRNA profiles of rice anthers between cool-sensitive and cool-tolerant cultivars under cool-temperature stress. (2016)
  • Author(s): Maeda, S., Sakazono, S., Masuko-Suzuki, H., Taguchi, M., Yamamura, K., Nagano, K., Endo, T., Saeki, K., Osaka, M., Nabemoto, M., Ito, K., Kudo, T., Kobayashi, M., Kawagishi, M., Fujita, K., Nanjo, H., Shindo, T., Yano, K., Suzuki, G., Suwabe, K., and Watanabe, M.
  • Journal Title: Genes Genet. Syst. Volume: -
  • NAID: 130005421208
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of reference genes for quantitative expression analysis using large scale RNA-seq data of Arabidopsis thaliana and model crop plants. (2016)
  • Author(s): Kudo T., Sasaki Y., Terashima S., Matsuda-Imai N., Takano T., Saito M., Kanno M., Ozaki S., Suwabe K., Suzuki G., Watanabe M., Matsuoka M., Takayama S., and Yano K.
  • Journal Title: Genes Genet. Syst. Volume: -
  • NAID: 130005421205
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transcriptional characteristics and differences in Arabidopsis stigmatic papilla cells pre- and post-pollination. (2015)
  • Author(s): Matsuda, T., Matsushima, M., Nabemoto, M., Osaka, M., Sakazono, S., Masuko-Suzuki, H., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K. K., Okumura, K., Suzuki, G., Watanabe, M., Suwabe, K.
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 4 Pages: 663-673
  • DOI: 10.1093/pcp/pcu209
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] アブラナ科野菜における分子育種の基盤構築とその応用. (2015)
  • Author(s): 諏訪部圭太
  • Journal Title: JATAFFジャーナル Volume: 3 Pages: 10-16
• [Journal Article] Plant omics data center: an integrated web repository for interspecies gene expression networks with NLP-based curation. (2015)
  • Author(s): Ohyanagi, H., Takano, T., Terashima, S., Kobayashi, M., Kanno, M., Morimoto, K., Matsumura, H., Sasaki, Y., Aya, K., Suwabe, K., Suzuki, G., Watanabe, M., Matsuoka, M., Yokoyama, K., Yano, K.
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 1 Pages: 1-8
  • DOI: 10.1093/pcp/pcu188
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] アブラナ科野菜における分子育種の基盤構築とその応用 (2015)
  • Author(s): 諏訪部圭太
  • Journal Title: JATAFFジャーナル Volume: 3
• [Journal Article] Physiological and Genetic Analysis of CO_2-Induced Breakdown of Self-Incompatibility in Brassica rapa (2014)
  • Author(s): Lao, X., Suwabe, K., Niikura, S., Kakita, M., Iwano, M., and Takayama, S.
  • Journal Title: J Exp Bot. Volume: 65 (4) Issue: 4 Pages: 939-951
  • DOI: 10.1093/jxb/ert438
  • Peer Reviewed
• [Journal Article] Time-lapse imaging of self- and cross-pollinations in Brassica rapa L.^<**>; Contributed equally to this work (2013)
  • Author(s): Hiroi, K., M. Sone, S. Sakazono, M. Osaka, H. Masuko-Suzuki, T. Matsuda, G. Suzuki, K. Suwabe and M. Watanabe
  • Journal Title: Annals of Botany Volume: 112 Issue: 1 Pages: 115-122
  • DOI: 10.1093/aob/mct102
  • Peer Reviewed
• [Journal Article] Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells from a Combination of Laser Microdissection and RNA Sequencing (2013)
  • Author(s): Osaka, M., Matsuda, T., Sakazono, S., Masuko-Suzuki, H., Maeda, S., Sewaki, M., Sone, M., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K.K., Yano, K., Lim, Y.P., Suzuki, G., Suwabe, K., and Watanabe, M.
  • Journal Title: Plant Cell Physiol. Volume: 54 (11) Issue: 11 Pages: 1894-1906
  • DOI: 10.1093/pcp/pct133
  • Peer Reviewed
• [Journal Article] Demonstration <i>in vivo</i> of the role of <i>Arabidopsis</i> PLIM2 actin-binding proteins during pollination (2013)
  • Author(s): Sudo, K., Park, J.-I, Sakazono, S., Masuko-Suzuki, H., Osaka, M., Kawagishi, M., Fujita, K., Maruoka, M., Nanjo, H., Suzuki, G., Suwabe, K., Watanabe, M.
  • Journal Title: Genes & Genetic Systems Volume: 88 Issue: 5 Pages: 279-287
  • DOI: 10.1266/ggs.88.279
  • NAID: 130003390809
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed
• [Presentation] Functional analysis of pollen-expressed cysteine-rich protein CR3. (2016)
  • Author(s): Morimoto, H., Arakawa, C., Egusa, W., Masuko-Suzuki, H., Matsushima, M., Suzuki, G., Watanabe, M., and Suwabe, K.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] シロイヌナズナ雌しべの柱頭毛は周囲の湿度を感じて開花後に伸長する (2016)
  • Author(s): 武田征士, 落合功輝, 坂園聡美, 渡辺正夫, 諏訪部圭太
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] シロイヌナズナおよび作物植物のRNA-seqデータを用いた遺伝子発現解析用リファレンス遺伝子の探索 (2016)
  • Author(s): 工藤徹，佐々木陽平，寺島伸，松田(今井)典子，高野知之，齋藤美沙，菅野真麻，諏訪部圭太，鈴木剛，渡辺正夫，松岡誠，高山誠司，矢野健太郎
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Identification of Reference Genes for Quantitative Expression Analysis Using Large-scale RNA-seq Data of Arabidopsis thaliana and Model Crop Plants. (2016)
  • Author(s): Kudo, T., Sasaki, Y., Terashima, S., Matsuda, N., Takano, T., Saito, M., Kanno, M., Suwabe, K., Suzuki, G., Watanabe, M., Matsuoka, M., Takayama, S., and Yano, K.
  • Organizer: International Plant and Animal Genome XXIV
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2016-01-09
  • Int'l Joint Research
• [Presentation] 複数植物種の遺伝子発現ネットワーク情報と高信頼度機能アノテーション情報を搭載したWebデータベースPODC (2015)
  • Author(s): 寺島伸, 高野智之, 工藤徹, 菅野真麻, 齋藤美沙, 松田典子, 浅野さとみ, 尾崎崇一, 佐々木陽平, 小林正明, 横山幸治, 諏訪部圭太, 鈴木剛, 渡辺正夫, 松岡信, 大柳一, 矢野健太郎
  • Organizer: 日本育種学会第128回講演会
  • Place of Presentation: 新潟大学（新潟県新潟市）
  • Year and Date: 2015-09-11
• [Presentation] 複数植物種の遺伝子発現ネットワーク情報と高信頼度アノテーション情報を搭載したWeb データベースPODC (2015)
  • Author(s): 寺島伸, 高野智之, 工藤徹, 菅野真麻, 齋藤美沙, 松田(今井)典子, 浅野さとみ, 佐々木陽平, 小林正明, 横山幸治, 諏訪部圭太, 鈴木剛, 渡辺正夫, 松岡信, 大柳一, 矢野健太郎
  • Organizer: 第12回 日本ナス科コンソシアム(JSOL)年会
  • Place of Presentation: 明治大学（神奈川県川崎市）
  • Year and Date: 2015-09-04
• [Presentation] Identification of Reference Genes for Quantitative Expression Analysis in Broad Experimental Conditions Using Large-scale RNA-seq Data of Arabidopsis thaliana and Model Crop Plants. (2015)
  • Author(s): Kudo, T., Sasaki, Y., Terashima, S., Matsuda-Imai, N., Takano, T., Saito, M., Kanno, M., Ozaki, S., Suwabe, K., Suzuki, G., Watanabe, M., Mastuoka, M., Takayama, S., and Yano, K.
  • Organizer: 第12回 日本ナス科コンソシアム(JSOL)年会
  • Place of Presentation: 明治大学（神奈川県川崎市）
  • Year and Date: 2015-09-04
• [Presentation] The pollen-expressed cysteine-rich peptide CR3 involves in pollen germination and pollen tube growth. (2015)
  • Author(s): Morimoto, H., Arakawa, C., Matsushima, M., Suzuki, G., Watanabe, M. and Suwabe, K.
  • Organizer: 26th International Conference on Arabidopsis Research
  • Place of Presentation: Paris, France
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] Revival of self-incompatibility in Arabidposis thaliana. (2015)
  • Author(s): Matsushima, M., Morimoto, H., Hikota, Y., Okumura, K., Yano, K., Shimizu, K.K., Takayama, S., Suzuki, G., Watanabe, M. and Suwabe, K.
  • Organizer: 26th International Conference on Arabidopsis Research
  • Place of Presentation: Paris, France
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] Transcriptional chagnes during pollination in Arabidopsis thaliana. (2015)
  • Author(s): Matsushima, M., Morimoto, H., Hikota, Y., Yano, K., Shimizu, K. K., Takayama, S., Suzuki, G., Watanabe, M., and Suwabe, K.
  • Organizer: International Symposium of Correlative Gene System, "Establishing Next-Generation Genetics"
  • Place of Presentation: 奈良春日野国際フォーラム甍（奈良県奈良市）
  • Year and Date: 2015-05-28
  • Int'l Joint Research
• [Presentation] Current solutions and pipelines for high-throughput sequencing data analysis. (2015)
  • Author(s): Kobayashi, M., Takano, T., Aya, K., Yano, K., Futagoishi, R., Niimi, T., Suwabe, K., Watanabe, M., Suzuki, G., Fujiwara, T., Matsuoka, M., Yano, K.
  • Organizer: International Symposium of Correlative Gene System, "Establishing Next-Generation Genetics"
  • Place of Presentation: 奈良春日野国際フォーラム甍（奈良県奈良市）
  • Year and Date: 2015-05-28
  • Int'l Joint Research
• [Presentation] Data mining with Plant Omics Data Center reveals conserved gene expression networks of molecular chaperone and protein disulfide isomerase genes in different organs. (2015)
  • Author(s): Kudo, T., Takano, T., Ohyanagi, H., Terashima, S., Kanno, M., Saito, M., Matsuda, N., Kobayashi, M., Ozaki, S., Sasaki, Y., Asano, S., Yokoyama, K., Aya, K., Suwabe, K., Suzuki, G., Watanabe, M., Matsuoka, M., Yano, K.
  • Organizer: International Symposium of Correlative Gene System, "Establishing Next-Generation Genetics"
  • Place of Presentation: 奈良春日野国際フォーラム甍（奈良県奈良市）
  • Year and Date: 2015-05-28
  • Int'l Joint Research
• [Presentation] Plant Omics Data Center: an integrated web repository for interspecies gene expression networks with NLP-based curation. (2015)
  • Author(s): Terashima, S., Takano, T., Ohyanagi, H.,, Kubo, T., Kanno, M., Saito, M., Matsuda, N., Kobayashi, M., Ozaki, S., Sasaki, Y., Asano, S., Yokoyama, Aya, K., Suwabe, K., Suzuki, G., Watanabe, M., Matsuoka, M., and Yano, K.
  • Organizer: International Symposium of Correlative Gene System, "Establishing Next-Generation Genetics"
  • Place of Presentation: 奈良春日野国際フォーラム甍（奈良県奈良市）
  • Year and Date: 2015-05-28
  • Int'l Joint Research
• [Presentation] Current progress of the pollination biology research. (2015)
  • Author(s): Suwabe, K.
  • Organizer: KSM spring meeting, KSM-ICWG-GSP joint clubroot symposium & Scientific seminar
  • Place of Presentation: Daejeon and Suncheon, Korea
  • Year and Date: 2015-05-15
  • Int'l Joint Research / Invited
• [Presentation] LM-RNA-seq による受粉時のシロイヌナズナ雌性生殖組織の機能解析 (2015)
  • Author(s): 松田智貴, 松嶋舞, 辺本萌, 大坂正明, 坂園聡美, 増子(鈴木)潤実, 曽根美佳子, 高橋宏和, 中園幹生, 岩野恵, 高山誠司, 清水健太郎, 奥村克純, 矢野健太郎, 鈴木剛, 渡辺正夫, 諏訪部圭太
  • Organizer: 日本育種学会第127回講演会
  • Place of Presentation: 玉川大学
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] 遺伝子発現ネットワーク情報と高精度アノテーション情報を搭載したWeb データベースPODCの開発 (2015)
  • Author(s): 髙野知之, 寺島伸, 工藤徹, 菅野真麻, 森本恭子, 鐘ヶ江弘美, 佐々木陽平, 小林正明, 尾崎崇一, 齋藤美沙, 浅野さとみ, 横山幸治, 安益公一朗, 諏訪部圭太, 鈴木剛, 渡辺正夫, 松岡信, 大柳一, 矢野健太郎
  • Organizer: 日本育種学会第127回講演会
  • Place of Presentation: 玉川大学
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] 植物オミクス統合データベースPlant Omics DataCenter の横断的遺伝子発現ネットワーク情報を用いたデータマイニング例 (2015)
  • Author(s): 工藤徹, 高野知之, 寺島伸, 菅野真麻, 森本恭子, 鐘ヶ江弘美, 尾崎崇一, 佐々木陽平, 齋藤美沙, 浅野さとみ, 横山幸治, 安益公一郎, 諏訪部圭太, 鈴木剛, 渡辺正夫, 松岡信, 小林正明, 大柳一, 矢野健太郎
  • Organizer: 日本育種学会第127回講演会
  • Place of Presentation: 玉川大学
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Comparative analysis of self-incompatibility factors in genus Arabidopsis. (2015)
  • Author(s): M. Matsushima, T. Matsuda, K. Nagasaka, C. Hoshiai, S. Narita, M. Nabemoto, S. Sakazono, H. Masuko-Suzuki, K. Yano, K.K. Shimizu, S. Takayama, K. Okumura, G. Suzuki, M. Watanabe and K. Suwabe
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Plant Omics Data Center (PODC): The integrated web repository for interspecies gene expression networks. (2015)
  • Author(s): T. Takano, S. Terashima, H. Ohyanagi, M. Kanno, K. Morimoto, H. Kanegae, Y. Sasaki, M. Kobayashi, T. Kudo, S. Asano, M. Saito, K. Yokoyama, K. Aya, K. Suwabe, G. Suzuki, M. Watanabe, M. Matsuoka and K. Yano
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Practical uses of PODC (Plant Omics Data Center): For discovering new gene association in expression data and NLP-based knowledge data. (2015)
  • Author(s): S. Asano, T. Takano, S. Terashima, M. Kobayashi, M. Kanno, K. Morimoto, H. Kanegae, S. Ozaki, Y. Sasaki, M. Saito, T. Kudo, K. Yokoyama, K. Aya, K. Suwabe, G. Suzuki, M. Watanabe, M. Matsuoka, H. Ohyanagi and K. Yano
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Data mining in Plant Omics Data Center: A case of molecular chaperone and protein disulfide isomerase genes. (2015)
  • Author(s): T. Kudo, T. Takano, S. Terashima, M. Kobayashi, M. Kanno, K. Morimoto, H. Kanegae, S. Ozaki, Y. Sasaki, M. Saito, S. Asano, K. Yokoyama, K. Aya, K. Suwabe, G. Suzuki, M. Watanabe, M. Matsuoka, H. Ohyanagi and K. Yano
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Comparative transcriptome analysis between pre- and post-pollination in Arabidposis thaliana. (2015)
  • Author(s): T. Matsuda, M. Matsushima, M. Nabemoto, M. Osaka, S. Sakazono, H. Masuko-Suzuki, M. Sone, H. Takahashi, M. Nakazono, M. Iwano, S. Takayama, K.K. Shimizu, K. Yano, G. Suzuki, M. Watanabe and K. Suwabe
  • Organizer: Plant & Animal Genome XXIII
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-01-10 – 2015-01-14
• [Presentation] Establishment of dynamic imaging of pollination in Arabidopsis thaliana. (2015)
  • Author(s): M. Matsushima, M. Ando, T. Matsuda, M. Nabemoto, M. Sone, K. Hiroi, S. Sakazono, H. Masuko-Suzuki, K. Yano, G. Suzuki, M. Watanabe and K. Suwabe
  • Organizer: Plant & Animal Genome XXIII
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-01-10 – 2015-01-14
• [Presentation] Data mining in Plant Omics Data Center suggests conserved gene expression networks of molecular chaperone and protein disulfide isomerase genes in different organs. (2015)
  • Author(s): T. Kudo, T. Takano, S. Terashima, M. Kobayashi, M. Kanno, K. Morimoto, H. Kanegae, S. Ozaki, Y. Sasaki, M. Saito, S. Asano, K. Yokoyama, K. Aya, K. Suwabe, G. Suzuki, M. Watanabe, M. Matsuoka, H. Ohyanagi and K. Yano
  • Organizer: Plant & Animal Genome XXIII
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-01-10 – 2015-01-14
• [Presentation] CA Plot Viewer and Plant Omics Data Center: A GUI-based Tool for Gene Expression Network Construction and an Integrated Web Repository for Interspecies Gene Expression Networks with NLP-based Curation. (2014)
  • Author(s): H. Ohyanagi, T. Takano, S. Terashima, M. Kobayashi, M. Kanno, K. Morimoto, H. Kanegae, S. Ozaki, T. Kudo, H. Matsumura, Y. Sasaki, M. Saito, S. Asano, K. Yokoyama, K. Aya, K. Suwabe, G. Suzuki, K.Aoki, Y. Kubo, M. Watanabe, M. Matsuoka and K. Yano
  • Organizer: 12th International Symposium on Rice Functional Genomics
  • Place of Presentation: Tucson, USA
  • Year and Date: 2014-11-16 – 2014-11-19
• [Presentation] 遺伝子発現ネットワークの種間比較を実現する植物オミクス統合データベースPODCを用いたデータマイニング例 (2014)
  • Author(s): 工藤徹，髙野知之，寺島伸，小林正明，森本恭子，菅野真麻，鐘ヶ江弘美，尾崎崇一，松村駿斗，佐々木 陽平，齋藤美沙，浅野さとみ，横山幸治，安益公一朗，諏訪部圭太，鈴木剛，青木考，久保康隆，渡辺正夫，松岡信，大柳一，矢野健太郎
  • Organizer: 11th JSOL International Symposium on Solanaceae Genomics
  • Place of Presentation: 名古屋大学
  • Year and Date: 2014-10-25 – 2014-10-26
• [Presentation] PODC活用例；トマトの遺伝子発現情報に基づくゲノム遺伝子相関の解明に向けて (2014)
  • Author(s): 浅野さとみ，髙野知之，寺島伸，小林正明，森本恭子，菅野真麻，鐘ヶ江弘美，尾崎崇一，工藤徹，佐々木 陽平，齋藤美沙，横山幸治，安益公一朗，諏訪部圭太，鈴木剛，青木考，久保康隆，渡辺正夫，松岡信，大柳一，矢野健太郎
  • Organizer: 11th JSOL International Symposium on Solanaceae Genomics
  • Place of Presentation: 名古屋大学
  • Year and Date: 2014-10-25 – 2014-10-26
• [Presentation] 遺伝子発現ネットワークの種間比較と高信頼度アノテーションの統合データベースPODC (2014)
  • Author(s): 高野知之, 小林正明, 大柳一, 佐々木陽平, 寺島伸, 松村駿斗, 森本恭子, 菅野真麻, 横山幸治, 千葉洋, 多田欣史, 清水顕史, 安益公一郎, 松岡信, 渡辺正夫, 諏訪部圭太, 矢野健太郎
  • Organizer: 日本育種学会第126回講演会
  • Place of Presentation: 南九州大学
  • Year and Date: 2014-09-26 – 2014-09-27
• [Presentation] 遺伝子発現ネットワークの種間比較と高信頼度アノテーションの統合データベース構築 (2014)
  • Author(s): 髙野知之, 寺島伸, 小林正明, 佐々木陽平, 松村駿人, 森本恭子, 菅野真麻, 千葉洋, 多田欣史, 清水顕史, 安益公一朗, 松岡信, 渡辺正夫, 諏訪部圭太,矢野健太郎
  • Organizer: 第32回日本植物細胞分子生物学会
  • Place of Presentation: 岩手県盛岡市
  • Year and Date: 2014-08-21 – 2014-08-22
• [Presentation] Cell type-specific RNA sequencing of papilla cell, a surface cell of pistil, by a combination of laser microdissection and next generation sequencing technologies. (2014)
  • Author(s): K. Suwabe
  • Organizer: The 2nd Plant Genomics Congress
  • Place of Presentation: London, UK
  • Year and Date: 2014-05-12 – 2014-05-13
  • Invited
• [Presentation] Cell type-specific transcriptome of Cruciferous stigmatic papilla cells from a combination of laser microdissection and RNA sequencing. (2014)
  • Author(s): Osaka, M., Matsuda, T., Sakazono, S., Masuko-Suzuki, H., Maeda, S., Sewaki, M., Sone, M., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K. K., Yano, K., Lim, Y.-P., Suzuki, G., Suwabe, K., Watanabe, M.
  • Organizer: Plant and Animal Genome XXII
  • Place of Presentation: San Diego, CA, USA
  • Invited
• [Presentation] 大規模オミックス情報を活用した網羅的な遺伝子発現ネットワーク構築と種間比較解析 (2014)
  • Author(s): 小林 正明, 高野 知之, 鈴木 貴之, 佐々木 陽平, 寺島 伸, 松村 駿斗, 森本 恭子, 菅野 真麻, 鐘ヶ江 弘美, 横山 幸治, 吉田 有希, 千葉 洋,多田 欣史, 清水 顕史, 安益 公一郎, 松岡 信, 渡辺 正夫, 諏訪部 圭太, 矢野 健太郎
  • Organizer: 第125回日本育種学会講演会
  • Place of Presentation: 東北大学
• [Presentation] Brachypodium distachyonにおいてmiR164bが雄性生殖器官の発達に及ぼす影響 (2014)
  • Author(s): 大嶋 涼達，前田 隼輔，坂園 聡美，増子 潤美，藤岡 智明，鈴木 剛，諏訪部 圭太，渡辺 正夫
  • Organizer: 第125回日本育種学会講演会
  • Place of Presentation: 東北大学
• [Presentation] Comprehensive analysis of the Arabidopsis stigmatic papilla cell transcriptome. (2013)
  • Author(s): Matsuda, T., Osaka, M., Sakasono, S., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Suzuki, G., Watanabe, M., Suwabe, K.
  • Organizer: 24th International Conference on Arabidopsis Research (Arabidopsis 2013)
  • Place of Presentation: Sydney, Australia
• [Presentation] The Arabidopsis stigmatic cell transcriptome by LM-NGS. (2013)
  • Author(s): Matsuda, T., Osaka, M., Sewaki, M., Sakasono, S., Suzuki, G., Watanabe, M., Suwabe, K.
  • Organizer: Plant Biology 2013
  • Place of Presentation: Providence, RI, USA
• [Presentation] Characterization of pollen behavior on self- and cross-pollination in Brassica rapa. (2013)
  • Author(s): Sone, M., Hiroi, K., Sakazono, S., Osaka, M., Masuko-Suzuki, H., Matsuda, T., Suzuki, G., Suwabe, K., Watanabe, M.
  • Organizer: Plant Biology 2013
  • Place of Presentation: Providence, RI, USA
• [Presentation] Arabidopsis属植物における受粉前後の発現遺伝子の比較解析 (2013)
  • Author(s): 松田智貴, 大坂正明, 瀬脇みさと, 坂園聡美, 長坂香里, 曽根美佳子, 増子(鈴木)潤美, 岩野恵, 高山誠司, 矢野健太郎, 鈴木剛, 渡辺正夫, 諏訪部圭太
  • Organizer: 第124回日本育種学会講演会
  • Place of Presentation: 鹿児島大学
• [Presentation] モデル植物のオミクス情報を活用した遺伝子発現ネットワークの大規模な種間比較解析 (2013)
  • Author(s): 小林正明, 高野知之, 菊江佳世子, 鈴木貴之, 佐々木陽平, 寺島伸, 松村駿斗, 吉岡幸江, 森本恭子, 菅野真麻, 横山幸治, 吉田有希, 千葉洋, 多田欣史, 清水顕史, 安益公一郎, 松岡信, 渡辺正夫, 諏訪部圭太, 矢野健太郎
  • Organizer: 第124回日本育種学会講演会
  • Place of Presentation: 鹿児島大学
• [Presentation] アブラナ科植物における自家および他家交配時の花粉動態解析 (2013)
  • Author(s): 曽根美佳子, 広井健一郎, 坂園聡美, 大坂正明, 鈴木(増子)潤美, 松田智貴, 鈴木剛, 諏訪部圭太, 渡辺正夫
  • Organizer: 第124回日本育種学会講演会
  • Place of Presentation: 鹿児島大学