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Effects of an active transposable element mPing on the rice epigenome and its application to plant breeding

Research Project

Project/Area Number 25292006
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionKindai University (2016)
Kyoto University (2013-2015)

Principal Investigator

TSUKIYAMA Takuji  近畿大学, 農学部, 准教授 (00423004)

Co-Investigator(Kenkyū-buntansha) 奥本 裕  京都大学, (連合)農学研究科(研究院), 教授 (90152438)
寺石 政義  京都大学, (連合)農学研究科(研究院), 講師 (80378819)
齊藤 大樹  京都大学, (連合)農学研究科(研究院), 助教 (10536238)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords転移因子 / イネ / エピジェネティクス / エピジェネティック
Outline of Final Research Achievements

In this study, we found that (1) a rice active transposon mPing alters transcript structures post-transcriptionally via induction of alternative splicing that most likely depends on DNA methylation, (2) a Myb-like protein encoded by the autonomous element Ping can bind to mPing sequence independently of DNA methylation, and (3) early embryogenesis-specific expression of Ping may be regulated by a SNP in Ping-ORF1 or position- and dosage-effect of Ping. These indicate that mPing can amplify in the rice genome by escaping from the epigenetic regulation and consequently contributes to the enlargement of genetic variability of the host genome.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (12 results)

All 2016 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results,  Open Access: 2 results) Presentation (7 results)

  • [Journal Article] The active miniature inverted-repeat transposable element mPing posttranscriptionally produces new transcriptional variants in the rice genome2015

    • Author(s)
      Rise Kum, Takuji Tsukiyama, Haruka Inagaki, Hiroki Saito, Masayoshi Teraishi, Yutaka Okumoto, Takatoshi Tanisaka
    • Journal Title

      Molecular Breeding

      Volume: 35 Issue: 8 Pages: 159-159

    • DOI

      10.1007/s11032-015-0353-y

    • NAID

      120005674594

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] イネユビキチン様タンパク質遺伝子Rurm1がメチルビオロゲンによって誘導される酸化ストレス応答におよぼす効果2015

    • Author(s)
      河西恵、築山拓司、寺石政義、奥本裕、谷坂隆俊
    • Journal Title

      作物研究

      Volume: 60 Pages: 55-57

    • NAID

      110010039623

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] トランスポゾンによる変異創生とその育種への応用2015

    • Author(s)
      金澤章、深井英吾、宮尾安藝雄、佐瀬英俊、築山拓司
    • Journal Title

      育種学研究

      Volume: 17 Pages: 77-87

    • Related Report
      2015 Annual Research Report
    • Open Access
  • [Journal Article] Early embryogenesis-specific expression of the rice transposon Ping enhances amplification of the MITE mPing2014

    • Author(s)
      Teramoto S., Tsukiyama T., Okumoto Y., Tanisaka T.
    • Journal Title

      PLOS Genetics

      Volume: 未定

    • NAID

      120005458284

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transient Dual-Luciferase Assay Combined with a Glucocorticoid-Inducible System for Rice Protoplasts2013

    • Author(s)
      Hamamoto Y., Tsukiyama T., Yoshitake Y., Teraishi M., Okumoto Y., Tanisaka T.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 12 Pages: 2480-2482

    • DOI

      10.1271/bbb.130480

    • NAID

      130003381948

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] イネゲノムにおける転移因子を介した選択的スプライシング2016

    • Author(s)
      築山拓司、琴梨世、稲垣晴香、齊藤大樹、寺石政義、奥本裕、谷坂隆俊
    • Organizer
      イネ遺伝学・分子生物学ワークショップ2016
    • Place of Presentation
      名古屋大学
    • Year and Date
      2016-07-04
    • Related Report
      2016 Annual Research Report
  • [Presentation] DNAメチル化がイネ自律性転移因子Pingの結合能におよぼす効果2016

    • Author(s)
      築山拓司、小野原一暢、田中幹也、齊藤大樹、奥本裕、寺石政義、井上國世、谷坂隆俊
    • Organizer
      一般社団法人日本育種学会第129回講演会
    • Place of Presentation
      横浜市立大学
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 活性型トランスポゾンを利用した新しい育種法の開発2014

    • Author(s)
      築山拓司
    • Organizer
      日本育種学会第126回講演会
    • Place of Presentation
      南九州大学
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] イネトランスポゾンmPingの転移活性を制御する因子の探索2014

    • Author(s)
      寺本翔太、築山拓司、谷坂隆俊、奥本裕
    • Organizer
      日本育種学会第126回講演会
    • Place of Presentation
      南九州大学
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Loss-of-function of an ubiquitin-related modifier RURM1 promotes the mobilization of the active MITE mPing2013

    • Author(s)
      Tsukiyama T., Teramoto S., Yasuda K., Horibata A., Mori N., Okumoto Y., Teraishi M., Saito H., Onishi A., Tamura K., Tanisaka T.
    • Organizer
      7th International Rice Genetic Symposium
    • Place of Presentation
      Manila
    • Related Report
      2013 Annual Research Report
  • [Presentation] イネ品種銀坊主におけるmPing転移は時期・組織特異的なPingの発現上昇を伴う2013

    • Author(s)
      寺本翔太、築山拓司、寺石政義、谷坂隆俊、奥本裕
    • Organizer
      日本育種学会第124回講演会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] イネRURM1によるtRNAの硫黄修飾を介したタ ンパク質翻訳効率の制御2013

    • Author(s)
      濵本有希、築山拓司、清水顕史、寺石政義、谷坂隆俊、奥本裕
    • Organizer
      日本育種学会第124回講演会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2020-01-20  

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