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Identification of the gametocidal gene that induce chromosome breakage in common wheat

Research Project

Project/Area Number 25292007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionKyoto University

Principal Investigator

Nasuda Shuhei  京都大学, (連合)農学研究科(研究院), 准教授 (10273492)

Research Collaborator FRIEBE Bernd  
AKHUNOV Eduard D.  
GILL Bikram S.  
HOUBEN Andreas  
DOLEZEL Jaroslav  
YOSHIOKA Motohiro  
MIZUNO Nobuyuki  
SAKAI Natsuto  
YAMADA Hajime  
MURATA Kazuki  
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywords配偶子致死 / マッピング / ラフマップ / 遺伝学 / 生殖 / 雑種不稔 / コムギ / 遺伝子単離 / ゲノム編集 / 機能解析 / 染色体切断
Outline of Final Research Achievements

The objective of this study is to identify the gametocidal gene that induce chromosome breakage in wheat. We took the approach of map-based cloning to identify the gene. First, we roughly mapped the gene to the tip of the long arm of chromosme T4B-4Ssh. Then, by adopting molecular markers of the syntenic chromosomal region of rice, we could narrow down the chromosomal region harboring the gametocidal gene to a 15 cM interval between two markers. It may give an impression that the gene is still distant from the molecular markers, but eventually the region is physically estimated to be 1.3 Mb because of inflation of apparent recombination near the gene in the population tested here. Our estimation is that if we could identify the markers as close as 1.0 cM, the gene would be within 100 kb from the markers. The results of the present study will promote identification and cloinig of the causal gene.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (12 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (5 results) Journal Article (2 results) (of which Open Access: 1 results) Presentation (5 results)

  • [Int'l Joint Research] Kansas State University(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Institute of Experimental Botany(チェコ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] IPK, Gatersleben(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] カンザス州立大学(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] 応用植物学研究所(チェコ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Chromosome 3B of Chinese Spring wheat is not a prerequisite for the gametocidal action of the Gc3-C1 gene2014

    • Author(s)
      Shota Watanabe, Takashi R. Endo, and Shuhei Nasuda
    • Journal Title

      eWIS: electronic - Wheat Information Service

      Volume: 117 Pages: 1-3

    • Related Report
      2014 Annual Research Report
    • Open Access
  • [Journal Article] Chromosome 3B of Chinese Spring wheat is not a prerequisite for the gametocidal action of the Gc3-C1 gene2014

    • Author(s)
      Shota Watanabe, Takashi R. Endo, Shuhei Nasuda
    • Journal Title

      Wheat Information Service

      Volume: 117 Pages: 1-3

    • Related Report
      2013 Annual Research Report
  • [Presentation] 配偶子致死遺伝子による接合体における染色体切断作用の解析2017

    • Author(s)
      山田創、那須田周平
    • Organizer
      日本育種学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] パンコムギにおける配偶子致死遺伝子Gc2-4Sshの遺伝的マッピング2016

    • Author(s)
      吉岡資洋、水野信之、酒井那乙斗、Bernd Friebe、那須田周平
    • Organizer
      日本育種学会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] パンコムギの配偶子致死遺伝子Gc2-4SshのRadiation Hybridマッピング集団の育成2016

    • Author(s)
      酒井那乙斗、吉岡資洋、水野信之、那須田周平
    • Organizer
      日本育種学会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Aegilops triunicialis に由来する del(3CL) 染色体の サーベイシークエンス2015

    • Author(s)
      水野 信之、田中 剛、Jan Vrana、Marie Kubalakova、Dolezel Jaroslav、遠藤 隆、 那須田 周平
    • Organizer
      日本育種学会 第127回講演会
    • Place of Presentation
      玉川大学
    • Year and Date
      2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] コムギにおける配偶子致死関連遺伝子 のradiation hybrid mapping2014

    • Author(s)
      那須田 周平、渡邊 将太、五十嵐 太郎、吉岡 資洋、辻本 壽
    • Organizer
      日本遺伝学会 第86回大会
    • Place of Presentation
      長浜バイオ大
    • Year and Date
      2014-09-17
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-21   Modified: 2025-01-30  

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