• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Cloning of hybrid incompatibility genes inhibiting artificial production of common wheat

Research Project

Project/Area Number 25292008
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionKobe University

Principal Investigator

Takumi Shigeo  神戸大学, (連合)農学研究科(研究院), 准教授 (50249166)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywords育種学 / 生殖隔離 / 倍数性進化 / コムギ / 高密度連鎖地図
Outline of Final Research Achievements

In crop breeding, efficient transmission of agriculturally important genes from wild relatives is necessary for overcoming difficult missions. However, technical problems such as reproductive isolation between crops and their relatives make it difficult to use comprehensively the genetic resources in wild relative populations. Towards complete use of a wild wheat relative Aegilops tauschii for bread wheat breeding, the goal of this research is set as map-based cloning of Net2 and Hch1, which are the D-genome causal genes for hybrid incompatibilities shown in interspecific hybrids from crosses between tetraploid wheat and Aegilops tauschii. Through this project, fine maps for Net2 on chromosome 2DS and Hch1 on chromosome 7DS were successfully constructed, and some BAC clones around the Net2 and Hch1 regions were selected. These progresses are important for further map-based cloning of Net2 and Hch1.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (49 results)

All 2016 2015 2014 2013 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results,  Acknowledgement Compliant: 10 results,  Open Access: 6 results) Presentation (26 results) (of which Invited: 3 results) Remarks (6 results)

  • [Journal Article] Comparison of gene expression profiles and responses to zinc chloride among inter- and intraspecific hybrids with growth abnormalities in wheat and its relatives2015

    • Author(s)
      Kiyofumi Takamatsu, Julio C.M. Iehisa, Ryo Nishijima and Shigeo Takumi
    • Journal Title

      Plant Molecular Biology

      Volume: 88 Issue: 4-5 Pages: 487-502

    • DOI

      10.1007/s11103-015-0338-6

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Quantitative trait locus analysis for flowering-related traits using two F<sub>2</sub> populations derived from crosses between Japanese common wheat cultivars and synthetic hexaploids2015

    • Author(s)
      Anh T. Nguyen, Ryo Nishijima, Tomonori Kajimura, Koji Murai and Shigeo Takumi
    • Journal Title

      Genes & Genetic Systems

      Volume: 90 Issue: 2 Pages: 89-98

    • DOI

      10.1266/ggs.90.89

    • NAID

      130005098890

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Implications of an inverted duplication in the wheat <i>KN1</i>-type homeobox gene <i>Wknox1</i> for theorigin of Persian wheat2015

    • Author(s)
      Shigeo Takumi and Ryoko Morimoto
    • Journal Title

      Genes & Genetic Systems

      Volume: 90 Issue: 2 Pages: 115-120

    • DOI

      10.1266/ggs.90.115

    • NAID

      130005098891

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Fine mapping of <i>Hch1</i>, the causal D-genome gene for hybrid chlorosis in interspecific crosses between tetraploid wheat and <i>Aegilops tauschii</i>2015

    • Author(s)
      Kana Hirao, Ryo Nishijima, Kohei Sakaguchi and Shigeo Takumi
    • Journal Title

      Genes & Genetic Systems

      Volume: 90 Issue: 5 Pages: 283-291

    • DOI

      10.1266/ggs.15-00035

    • NAID

      130005138533

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Extracellular trafficking of a wheat cold-responsive protein, WLT102015

    • Author(s)
      Ryoko Ohno and Shigeo Takumi
    • Journal Title

      Journal of Plant Physiology

      Volume: 174 Pages: 71-74

    • DOI

      10.1016/j.jplph.2014.10.004

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Line differences in Cor/Lea and fructan biosynthesis-related gene transcript accumulation are related to distinct freezing tolerance levels in synthetic wheat hexaploids2015

    • Author(s)
      Hirokazu Yokota, Julio C.M. Iehisa, Etsuo Shimosaka and Shigeo Takumi
    • Journal Title

      Journal of Plant Physiology

      Volume: 176 Pages: 78-88

    • DOI

      10.1016/j.jplph.2014.12.007

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Accelerated senescence and enhanced disease resistance in hybrid chlorosis lines derived from interspecific crosses between tetraploid wheat and Aegilops tauschii2015

    • Author(s)
      Hiroki Nakano, Nobuyuki Mizuno, Yukio Tosa, Kentaro Yoshida, Pyoyun Park and Shigeo Takumi
    • Journal Title

      PLoS ONE

      Volume: 10 Issue: 3 Pages: e0121583-e0121583

    • DOI

      10.1371/journal.pone.0121583

    • NAID

      120005603830

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of quantitative trait loci for abscisic acid responsiveness in the D-genome of hexaploid wheat2014

    • Author(s)
      Julio C.M. Iehisa, Takakazu Matsuura, Izumi C. Mori, Hirokazu Yokota, Fuminori Kobayashi and Shigeo Takumi
    • Journal Title

      Journal of Plant Physiology

      Volume: 171 Issue: 10 Pages: 830-841

    • DOI

      10.1016/j.jplph.2014.02.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A high-density genetic map with array-based markers facilitates structural and quantitative trait locus analyses of the common wheat genome2014

    • Author(s)
      Julio C.M. Iehisa, Ryoko Ohno, Tatsuro Kimura, Hiroyuki Enoki, Satoru Nishimura, Yuki Okamoto, Shuhei Nasuda and Shigeo Takumi
    • Journal Title

      DNA Research

      Volume: 21 Issue: 5 Pages: 555-567

    • DOI

      10.1093/dnares/dsu020

    • NAID

      120005603827

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The cuticular wax inhibitor locus Iw2 in wild diploid wheat Aegilops tauschii: phenotypic survey, genetic analysis, and implications for the evolution of common wheat2014

    • Author(s)
      Ryo Nishijima, Julio C.M. Iehisa, Yoshihiro Matsuoka and Shigeo Takumi
    • Journal Title

      BMC Plant Biology

      Volume: 14 Issue: 1 Pages: 246-246

    • DOI

      10.1186/s12870-014-0246-y

    • NAID

      120005603829

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of quantitative trait locus for abscisic acid responsiveness on chromosome 5A and association with dehydration tolerance in common wheat seedlings2014

    • Author(s)
      Julio C.M. Iehisa, Takakazu Matsuura, Izumi C. Mori, Shigeo Takumi
    • Journal Title

      Journal of Plant Physiology

      Volume: 171 Pages: 25-34

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide marker development for the wheat D genome based on single nucleotide polymorphisms identified from transcripts in the wild wheat progenitor Aegilops tauschii.2014

    • Author(s)
      Iehisa M, Shimizu A, Sato K, Nishijima R, Sakaguchi K, Matsuda R, Nasuda S, Takumi S
    • Journal Title

      Theoretical and Applied Genetics

      Volume: 127 Issue: 2 Pages: 261-271

    • DOI

      10.1007/s00122-013-2215-5

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Segregation distortion caused by weak hybrid necrosis in recombinant inbred lines of common wheat2013

    • Author(s)
      Shigeo Takumi, Yoichi Motomura, Julio C.M. Iehisa, Fuminori Kobayashi
    • Journal Title

      Genetica

      Volume: 141 Issue: 10-12 Pages: 463-470

    • DOI

      10.1007/s10709-013-9745-2

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of quantitative trait loci controlling grain size and shape in the D genome of synthetic hexaploid wheat lines2013

    • Author(s)
      Yuki Okamoto, Anh T. Nguyen, Motohiro Yoshioka, Julio C.M. Iehisa, Shigeo Takumi
    • Journal Title

      Breeding Science

      Volume: 63 Issue: 4 Pages: 423-429

    • DOI

      10.1270/jsbbs.63.423

    • NAID

      130004146405

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential contribution of two <i>Ppd-1</i> homoeoalleles to early-flowering phenotype in Nepalese and Japanese varieties of common wheat2013

    • Author(s)
      Anh T. Nguyen, Julio C.M. Iehisa, Nobuyuki Mizuno, Miyuki Nitta, Shuhei Nasuda, Shigeo Takumi
    • Journal Title

      Breeding Science

      Volume: 63 Issue: 4 Pages: 374-383

    • DOI

      10.1270/jsbbs.63.374

    • NAID

      130004146399

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pleiotropic effects of the elongated glume gene P1 on grain and spikelet shape-related traits in tetraploid wheat2013

    • Author(s)
      Yuki Okamoto, Shigeo Takumi
    • Journal Title

      Euphytica

      Volume: 194 Issue: 2 Pages: 207-218

    • DOI

      10.1007/s10681-013-0916-0

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of quantitative trait loci for flowering-related traits in the D genome of synthetic hexaploid wheat lines.2013

    • Author(s)
      Nguyen TA, Iehisa JCM, Kajimura T, Murai K, Takumi S.
    • Journal Title

      Euphytica

      Volume: (in press) Issue: 3 Pages: 401-412

    • DOI

      10.1007/s10681-013-0873-7

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] RNA-seq解析によるコムギ近縁Uゲノム種Aegilops umbellulataのゲノムワイドな多型検出2016

    • Author(s)
      岡田萌子、吉田健太郎、西嶋遼、佐藤和宏、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      横浜市立大学(神奈川県横浜市)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] RNA-seqデータに基づいた合成パンコムギDゲノム上の特定領域へのタルホコムギscaffoldsの補充2016

    • Author(s)
      西嶋遼、坂口晃平、吉田健太郎、水野信之、那須田周平、佐藤和宏、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      横浜市立大学(神奈川県横浜市)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 野生一粒系コムギ雑種種子の発生異常2015

    • Author(s)
      宅見薫雄
    • Organizer
      ムギ類研究会
    • Place of Presentation
      伊勢市観光文化会館(三重県伊勢市)
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] RNA-seq解析によるタルホコムギゲノムワイドマーカーの検出2015

    • Author(s)
      西嶋遼、吉田健太郎、佐藤和広、宅見薫雄
    • Organizer
      ムギ類研究会
    • Place of Presentation
      伊勢市観光文化会館(三重県伊勢市)
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞死に関与するコムギWRKY転写因子を発現した形質転換シロイヌナズナの解析2015

    • Author(s)
      久木康伸、大野良子、吉田健太郎、宅見薫雄
    • Organizer
      ムギ類研究会
    • Place of Presentation
      伊勢市観光文化会館
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 低温誘導性雑種致死を誘発するタルホコムギNet2のfine mapping2015

    • Author(s)
      坂口晃平、宅見薫雄
    • Organizer
      ムギ類研究会
    • Place of Presentation
      伊勢市観光文化会館(三重県伊勢市)
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] コムギ・エギロプス属のゲノム間隔離障壁と雑種不全2015

    • Author(s)
      宅見薫雄
    • Organizer
      第29回木原セミナー
    • Place of Presentation
      横浜市立大学木原生物学研究所(神奈川県横浜市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] タルホコムギ10系統の幼苗葉のRNA-seqデータからのSNPの検出2015

    • Author(s)
      西嶋遼、吉田健太郎、佐藤和広、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      新潟大学(新潟県新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] コムギ種間雑種における生育不全で発現量が増加するWRKY転写因子の解析2015

    • Author(s)
      久木康伸、大野良子、吉田健太郎、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      新潟大学(新潟県新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] タルホコムギの葉と幼穂のRNA-seqデータに基づく選択的スプライシング産物の検出2015

    • Author(s)
      宅見薫雄、Julio C.M. Iehisa、岡田萌子、佐藤和広
    • Organizer
      日本育種学会
    • Place of Presentation
      新潟大学(新潟県新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 2倍体コムギ近縁種Aegilops umbellulataの二粒系コムギとの交雑親和性に関する種内変異の解析2015

    • Author(s)
      岡田萌子、吉田健太郎、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      新潟大学(新潟県新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] ゲノムの分化を越えられるか―コムギ・エギロプス属の人為種間交雑2015

    • Author(s)
      宅見薫雄
    • Organizer
      研究集会「コムギ遺伝資源の利活用に関する研究打ち合わせ:乾燥地での栽培特性の向上をめざして」
    • Place of Presentation
      鳥取大学乾燥地研究センター(鳥取県鳥取市)
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 二粒系コムギとの雑種でハイブリッドクロロシスを誘導するタルホコムギ遺伝子Hch1の高密度連鎖地図の作成2015

    • Author(s)
      平尾佳奈、西嶋遼、坂口晃平、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      玉川大学
    • Year and Date
      2015-03-20 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 二粒系コムギとの雑種でタイプ2ネクローシスを誘導するタルホコムギ遺伝子Net2の高密度連鎖地図の作成2015

    • Author(s)
      坂口晃平、西嶋遼、Iehisa Masaru、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      玉川大学
    • Year and Date
      2015-03-20 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 二倍体野生コムギ2種の種間交雑により生じる異常種子のRNAseq解析2015

    • Author(s)
      高松清史、吉田健太郎、佐藤和広、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      玉川大学
    • Year and Date
      2015-03-20 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 野生一粒系コムギ2種の種間交雑によって形成される種子形態へのハイブリッドネクローシスの影響2014

    • Author(s)
      高松清史、池田達哉、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      南九州大学
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] タルホコムギのanathera変種が持つ芒抑制遺伝子周辺領域の染色体構造2014

    • Author(s)
      西嶋遼、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      南九州大学
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] タルホコムギが持つ早生遺伝子を日本のパンコムギへ導入できる可能性について2014

    • Author(s)
      宅見薫雄、Anh Tuan Nguyen、村井耕二
    • Organizer
      日本育種学会
    • Place of Presentation
      南九州大学
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 野生一粒系コムギにおけるハイブリッドネクローシスの遺伝子発現解析2014

    • Author(s)
      高松清史、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      東北大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] タルホコムギにおける帯白性の自然変異とパンコムギの起原2014

    • Author(s)
      西嶋遼、Iehisa Masaru、松岡由浩、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      東北大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] miR156 is associated with the grass clump phenotype in hybrids between tetraploid wheat and Aegilops tauschii2013

    • Author(s)
      Shigeo Takumi, Ryusuke Matsuda, Julio C.M. Iehisa
    • Organizer
      12th International Wheat Genetics Symposium
    • Place of Presentation
      Pacifico Yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] Application of SNPs derived from transcripts of two distinct Aegilops tauschii accessions to wheat genome analysis2013

    • Author(s)
      Julio C.M. Iehisa, Akifumi Shimizu, Ryo Nishijima, Kazuhiro Sato, Shuhei Nasuda, Shigeo Takumi
    • Organizer
      12th International Wheat Genetics Symposium
    • Place of Presentation
      Pacifico Yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comparison of gene expression profiles of hybrid necrosis in einkorn and common wheat2013

    • Author(s)
      Kiyofumi Takamatsu, Julio C.M. Iehisa, Shigeo Takumi
    • Organizer
      12th International Wheat Genetics Symposium
    • Place of Presentation
      Pacifico Yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] Mapping of a dominant awnless gene in Aegilops tauschii2013

    • Author(s)
      Ryo Nishijima, Shigeo Takumi
    • Organizer
      12th International Wheat Genetics Symposium
    • Place of Presentation
      Pacifico Yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] タルホコムギの葉と穂のRNA-seq解析から同定されたSNPの6倍体コムギ解析への利用2013

    • Author(s)
      Iehisa Masaru、清水顕史、佐藤和広、西嶋遼、坂口晃平、那須田周平、宅見薫雄
    • Organizer
      日本育種学会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] コムギ種間雑種にみられる低温誘導性ハイブリッドネクローシス2013

    • Author(s)
      宅見薫雄、松田龍典、Julio C.M. Iehisa、水野信之
    • Organizer
      日本育種学会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Remarks] 神戸大学植物遺伝学研究室HP

    • URL

      http://www.plantgenetics-kobeu.info/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 神戸大学農学部インターゲノミクス研究会HP

    • URL

      http://www.research.kobe-u.ac.jp/ans-intergenomics/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 神戸大学植物遺伝学研究室

    • URL

      http://www.plantgenetics-kobeu.info/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 神戸大学農学部インターゲノミクス研究会

    • URL

      http://www.research.kobe-u.ac.jp/ans-intergenomics/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 神戸大学農学部インターゲノミクス研究会

    • URL

      http://www.research.kobe-u.ac.jp/ans-intergenomics/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 神戸大学大学院農学研究科植物遺伝学研究室

    • URL

      http://www.lab.kobe-u.ac.jp/ans-plantgenetics/

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi