Identification of beneficial genes on non-recombining regions by using heavy-ion deletion mapping
| Project/Area Number |
25292009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Kazama Yusuke 国立研究開発法人理化学研究所, 仁科加速器研究センター, 協力研究員 (80442945)
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| Co-Investigator(Kenkyū-buntansha) |
YASUI YASUO 京都大学, 農学研究科, 助教 (70293917)
TAKAHARA MANABU 国立研究開発法人農業・食品産業技術総合研究機構, 畜産草地研究所, 主任研究員 (90355123)
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| Co-Investigator(Renkei-kenkyūsha) |
ABE TOMOKO 国立研究開発法人理化学研究所, 仁科加速器研究センター, 室長 (70201895)
ISHII KOTARO 国立研究開発法人理化学研究所, 仁科加速器研究センター, 特別研究員 (50632965)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 育種学 / 重イオンビーム / 組換え抑制領域 / 植物 / 生殖システム / 欠失マッピング |
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| Outline of Final Research Achievements |
Genes responsible for dioecy, self-incompatibility, and apomixis, which have been related to diversification of plant reproduction systems, are located on chromosomal non-recombining regions. Thus, it has been difficult to identify these genes. In this study, mutant lines having deletions on the non-recombining regions were developed. The deletion maps of the lines were calculated by using the newly developed software “DelMapper”, and used for narrowing down the positions of the responsible genes. Together with the information for genomes and transcriptomes obtained from next-generation sequencing, candidates for the responsible genes were successfully listed.
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Report
(4 results)
Research Products
(66 results)
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[Journal Article] A new physical mapping approach refines the sex-determining gene positions on the Silene latifolia Y-chromosome2016
Author(s)
Kazama Y, Ishii K, Aonuma W, Ikeda T, Kawamoto H, Koizumi A, Filatov DA, Chibalina M, Bergero R, Charlesworth D, Abe T, Kawano S
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 18917-18917
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Wild is sexy!2015
Author(s)
内藤健, 高橋有, 山内卓樹, 福島健児, 風間裕介, 江崎文一, 友岡憲彦
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Journal Title
Breeding Research
Volume: 17
Issue: 2
Pages: 64-70
DOI
NAID
ISSN
1344-7629, 1348-1290
Related Report
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[Journal Article] Diversification of 13S globulins, allergenic seed storage proteins, of common buckwheat2014
Author(s)
Sano, M., Nakagawa, M., Oishi, A., Yasui, Y., Katsube-Tanaka, T
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Journal Title
Food Chemistry
Volume: 155
Pages: 192-198
DOI
Related Report
Peer Reviewed
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[Journal Article] Phenotypic spectrum of Parachlorella kessleri (Chlorophyta) mutants produced by heavy-ion irradiation.2013
Author(s)
Ota, S., Matsuda, T., Takeshita, T., Yamazaki, T., Kazama, Y., Abe, T., Kawano, S.
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Journal Title
Bioresour. Technol.
Volume: 149
Pages: 432-438
DOI
Related Report
Peer Reviewed
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[Journal Article] Rapid degeneration of noncoding DNA regions surrounding SlAP3X/Y after recombination suppression in a dioecious plant, Silene latifolia.2013
Author(s)
Ishii, K., Nishiyama, R., Shibata, F., Kazama, Y., Abe, T., and Kawano, S.
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Journal Title
G3
Volume: 3
Issue: 12
Pages: 2121-2130
DOI
Related Report
Peer Reviewed
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[Presentation] 重イオンビームによるグリーンイノベーションの創出2015
Author(s)
風間裕介, 平野智也, 大田修平, 佐藤陽一, 村井耕二, 河野重行, 小野克徳, 阿部知子
Organizer
近畿作物・育種研究会公開シンポジウム 「変異創成技術の現状と未来」
Place of Presentation
京都教育大学藤森キャンパス(京都)
Year and Date
2015-12-12
Related Report
Invited
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