Mechanism of the maintenance of single nucleotide polymorphism endowing resistance to herbicide in weeds

• Project/Area Number: 25292013
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Crop production science
• Research Institution: Kyoto University
• Principal Investigator: TOMINAGA Tohru 京都大学, (連合)農学研究科(研究院), 教授 (10135551)
• Co-Investigator(Kenkyū-buntansha): OHDAN Hideki 独立行政法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター水田作・園芸研究領域, 主任研究員 (70343978)
• Co-Investigator(Renkei-kenkyūsha): SHIMONO Yoshiko 京都大学, 大学院農学研究科, 助教 (40469755)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000); Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 除草剤抵抗性 / 一塩基多型 / スズメノテッポウ / スルホニルウレア剤 / 雑草 / 作用点抵抗性

Outline of Final Research Achievements

Herbicide resistance in weeds is due to changes in target enzyme. In this research, single nucleotide polymorphisms in sulfonylurea herbicide resistant Alopecurus aequalis populations in north Kyushu, Japan were clarified. Eight single nucleotide substitutions were found in Pro197 and Trp574 in the ALS enzyme. The level of resistance to SU herbicide was the highest in ALS1Thr197 and ALS1Leu574 biotypes, middle in ALS1Ser197、ALS2Ser197、ALS1Ala197 and ALS1His197 biotypes, and the lowest in ALS1Leu197 and ALS2Leu197 biotypes.

Research Products

• [Journal Article] 雑草種子の生理・生態的特性 (2016)
  • Author(s): 冨永達
  • Journal Title: グリーンエイジ Volume: 16 Pages: 4-7
• [Journal Article] 雑草の除草剤抵抗性-そのメカニズムと生活史特性- (2015)
  • Author(s): 冨永達
  • Journal Title: 日本農薬学会誌 Volume: 40 Pages: 240-242
• [Journal Article] 雑草における農業生態型の分化 (2015)
  • Author(s): 冨永達
  • Journal Title: 植調 Volume: 49 Pages: 4-7
• [Presentation] Single Nucleotide Polymorphisms in sulfonylurea herbicide resistant Alopecurus aequalis populations in Japan (2015)
  • Author(s): T. Kiyoku, Y. Shimono, H. Ohdan and T. Tominaga
  • Organizer: Resistance 2015 Meeting
  • Place of Presentation: Rothamsted Research, England
  • Year and Date: 2015-09-14
  • Int'l Joint Research
• [Presentation] 九州北部で出現したスルホニルウレア系除草剤抵抗性スズメノテッポウにおけるALS遺伝子の集団内多型 (2015)
  • Author(s): 清久達裕・眞鍋陽平・大段秀記・下野嘉子・冨永達
  • Organizer: 雑草学会
  • Place of Presentation: 秋田県立大学（秋田市）
  • Year and Date: 2015-04-18 – 2015-04-19
• [Presentation] 九州北部のスルホニルウレア系除草剤抵抗性スズメノテッポウの作用点変異と抵抗性の程度 (2015)
  • Author(s): 眞鍋陽平・大段秀記・下野嘉子・冨永達
  • Organizer: 雑草学会
  • Place of Presentation: 秋田県立大学（秋田市）
  • Year and Date: 2015-04-18 – 2015-04-19
• [Presentation] 九州北部で出現したスルホニルウレア系除草剤抵抗性スズメノテッポウにおけるALS遺伝子の集団内多型 (2015)
  • Author(s): 清久達裕・眞鍋陽平・大段秀記・下野嘉子・冨永達
  • Organizer: 日本雑草学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2015-04-18
• [Presentation] 九州北部のスルホニルウレア系除草剤抵抗性スズメノテッポウの作用点変異と抵抗性の程度 (2015)
  • Author(s): 眞鍋陽平・大段秀記・下野嘉子・冨永達
  • Organizer: 日本雑草学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2015-04-18
• [Presentation] 雑草の除草剤抵抗性 －そのメカニズムと生活史特性－ (2015)
  • Author(s): 冨永達
  • Organizer: 農薬学会
  • Place of Presentation: 玉川大学（東京都町田市）
  • Year and Date: 2015-03-18
  • Invited
• [Book] 身近な雑草の生物学 (2014)
  • Author(s): 根本正之・冨永達
  • Total Pages: 151
  • Publisher: 朝倉書店