Effect of Polymorphism of Nitrogen Fixation Related Gene SEN1 on Soybean Production

• Project/Area Number: 25292014
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Crop production science
• Research Institution: Saga University
• Principal Investigator: Suzuki Akihiro 佐賀大学, 農学部, 教授 (50305108)
• Co-Investigator(Kenkyū-buntansha): 穴井 豊昭 佐賀大学, 農学部, 教授 (70261774) ; 有馬 進 佐賀大学, 農学部, 教授 (90140954)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: 根粒菌 / 窒素固定 / 共生 / ダイズ / ミヤコグサ / 遺伝子 / 多型 / 根粒 / sen1遺伝子 / マメ科植物

Outline of Final Research Achievements

We found polymorphism in amino acid sequence of soybean SEN1 genes. Nitrogen fixation activity of Lotus japonicus sen1 mutant transformed by Enrei SEN1 gene was slightly higher than that of Peking type SEN1 gene. Effect of polymorphism of soybean SEN1 gene on the soybean yield was analyzed using BC5F2 soybean plants prepared from crossing with Fukuyutaka (Peking type) and Enrei. As a result, 100-seed weight of Enrei type BC5F2 plants slightly higher than that of Peking type. These results show the possibility that Enrei type SEN1 gene have positive effect for soybean 100-seed weight due to higher nitrogen fixation in the nodules.

Research Products

• [Int'l Joint Research] カリフォルニア大学ロサンゼルス(米国)
• [Journal Article] 光質が根粒菌の感染を制御するーフィトクロムシグナリングを介した根粒形成のメカニズムー (2014)
  • Author(s): 永田真紀，鈴木章弘
  • Journal Title: 化学と生物 Volume: 52 Pages: 136-138
  • NAID: 130004808952
  • Peer Reviewed
• [Presentation] SEN1 遺伝子の多型がマメ科の表現型に与える影響 (2014)
  • Author(s): 河濟英紀 ,原田克哉 ,富永晃好,菅沼教生,橋口正嗣,明石良,渡邊哲史,穴井豊昭,有馬進,鈴木章弘
  • Organizer: 第2４回植物微生物研究会研究交流会
  • Place of Presentation: 佐賀
  • Year and Date: 2014-09-19 – 2014-09-20
• [Presentation] Both root nodulation and arbuscular mycorrhization are photomorphogenetically controlled by sensing the red/far red (R/FR) ration through jasmonic acid (JA) signaling. (2013)
  • Author(s): Akihiro Suzuki et al.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Effect of light irradiation to the root on Lotus japonicas root nodule formation (2013)
  • Author(s): Aya Shimomura et al.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Effect of the diversity of SEN1 gene on nitrogen fixation activity in Lotus japonicus (2013)
  • Author(s): Katsuya Harada et al.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Transcriptional analysis of an in vitro nitrogen fixation reaction induced by free living bacterial cells of B. japonicum USDA110 with symbio (2013)
  • Author(s): Tadashi Yokoyama et al.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Presentation] Control of mycorrhizal infection by Red/Far-Red (R/FR) ratio through jasmonic acid (JA) signaling. (2013)
  • Author(s): Akihiro Suzuki et al.
  • Organizer: Plant Biology 2013
  • Place of Presentation: Providence
• [Book] Chapter 6 of "Advances in Biology and Ecology of Nitrogen Fixation" : Effects of Phytohormones on Nodulation and Nitrogen Fixation in Leguminous Plants (2014)
  • Author(s): Maki Nagata and Akihiro Suzuki
  • Publisher: INTECH
• [Book] Chapter 57 of "Biological Nitrogen Fixation" : Lotus japonicus nodules when it sees red. (2014)
  • Author(s): Maki Nagata, Ann M. Hirsch and Akihiro Suzuki
  • Publisher: Wiley/Blackwell
• [Remarks] 佐賀大学農学部生物環境科学科作物生態生理学研究室ホームページ
  • URL: http://extwww.cc.saga-u.ac.jp/~azuki/top.html
• [Remarks] 佐賀大学農学部生物環境科学科作物生態生理学研究室ホームページ
  • URL: http://extwww.cc.saga-u.ac.jp/̃azuki/top.html