Molecular mechanism of floral diversification in orchids

• Project/Area Number: 25292018
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Tohoku University
• Principal Investigator: KANNO AKIRA 東北大学, 生命科学研究科, 准教授 (10260449)
• Co-Investigator(Kenkyū-buntansha): MII MASAHIRO 千葉大学, 環境健康フィールド科学センター, 特任研究員 (30093074) ; HANDA TAKASHI 明治大学, 農学部, 教授 (00192708) ; YUKAWA TOMOHISA 独立行政法人国立科学博物館, 筑波実験植物園, 研究主幹 (50280524) ; SATO SYUSEI 東北大学, 大学院生命科学研究科, 准教授 (70370921)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 遺伝子単離 / 遺伝子発現 / ゲノム解析 / 形質転換 / 変異体 / シグモルキス / サギソウ

Outline of Final Research Achievements

In order to understand the molecular mechanism of the orchid-specific floral organs such as lip, column, pollinium, we established the cultivation system and Agrobacterium-mediated transformation system in Psygmorchis pusilla. We also analyzed the optimal condition for mutant generation with ion beam and EMS. cDNA fragments related to floral development were isolated from Psygmorchis pusilla and Habenaria radiata.

Research Products

• [Journal Article] Phylogeny and classification of the East Asian Amitostigma alliance (Orchidaceae: Orchideae) based on six DNA markers (2015)
  • Author(s): Tang, Y., T. Yukawa, R. M. Bateman, H. Jiang and H. Peng
  • Journal Title: BMC Evolutionary Biology Volume: 15 Issue: 1 Pages: 96-96
  • DOI: 10.1186/s12862-015-0376-3
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Studies in Asian Nervilia (Orchidaceae) V: N. futago, a cryptic new species from southwest Japan confirmed by morphological, cytological and molecular analyses (2015)
  • Author(s): Gale, S. W., J. Li, A. Kinoshita and T. Yukawa
  • Journal Title: Systematic Botany Volume: 40 Issue: 2 Pages: 413-425
  • DOI: 10.1600/036364415x688772
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effects of NAA and BA on PLB growth of Psygmorchis pusilla. (2014)
  • Author(s): Kodama, T. and T. Handa
  • Journal Title: Acta Hort. Volume: 1025 Pages: 195-199
  • Peer Reviewed
• [Journal Article] Molecular phylogenetics and character evolution of morphologically diverse groups, Dendrobium section Dendrobium and allies. (2014)
  • Author(s): Takamiya, T., P. Wongsawad, A. Sathapattayanon, N. Tajima, S. Suzuki, S. Kitamura, N.Shioda, T. Handa, S. Kitanaka, H. Iijima and T. Yukawa
  • Journal Title: AoB Plants Volume: 6
  • Peer Reviewed
• [Journal Article] Acylated cyanidin 3,7-diglucosides in the red-purple flowers of Sophronitis wittigiana (Orchidaceae). (2014)
  • Author(s): Tatsuzawa, F., N. Saito, T. Yukawa, T. Honda, K. Shinoda, K. Kato and K. Miyoshi
  • Journal Title: Journal of the Japanese Society for Horticultural Science Volume: 83 Pages: 64-71
  • NAID: 40019948948
  • Peer Reviewed
• [Journal Article] Nomenclatural changes in the genus Calanthe (Orchidaceae). (2014)
  • Author(s): Yukawa, T. and P. J. Cribb
  • Journal Title: Bulletin of the National Museum of Nature and Science Series B (Botany) Volume: 40 Pages: 145-151
  • NAID: 40020318749
  • Peer Reviewed
• [Presentation] Diversity and conservation status of Japanese indigenous orchids. (2015)
  • Author(s): Yukawa, T., K. Suzuki and Y. Ogura-Tsujita
  • Organizer: 11th International Symposium on Diversity and Conservation of Asian Orchids (Janghang)
  • Place of Presentation: ソチョン郡（韓国）
  • Year and Date: 2015-12-16
  • Int'l Joint Research
• [Presentation] サギソウ獅子咲き形質に連鎖するDEF様遺伝子の同定 (2015)
  • Author(s): 梶野祐未、三苫舞、林里沙、菅野明
  • Organizer: 園芸学会平成27年度秋季大会
  • Place of Presentation: 徳島大学（徳島県徳島市）
  • Year and Date: 2015-09-26
• [Presentation] ラン科植物Erycina pusilla におけるTCP遺伝子の単離 (2015)
  • Author(s): 關根健人、菅野明、佐藤修正、半田高
  • Organizer: 第３３回日本植物細胞分子生物学会
  • Place of Presentation: 東京大学（東京）
  • Year and Date: 2015-08-10
• [Presentation] ラン科セッコク属の多様性解析に基づく薬用資源の探索. (2015)
  • Author(s): 曽根麻友美・藤原有紀子・町田智美・松本亮平・菊地秦平・清水玲子・吉野圭一・北中進・遊川知久・飯島洋・高宮知子
  • Organizer: 日本植物園協会第50回大会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2015-06-25
• [Presentation] ラン科セッコク属植物の包括的な情報構築と分類に基づく薬用資源の探索. (2014)
  • Author(s): 高宮知子・海保有芳・鶴牧友梨香・曽根麻友美・藤原有紀子・町田智美・松本亮平・北中進・飯島洋・遊川知久
  • Organizer: 第23回日本DNA多型学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-11-26 – 2014-11-28
• [Presentation] サギソウの花器官におけるDEFICIENS様遺伝子群の単離と発現解析 (2014)
  • Author(s): 三苫舞・林里沙・菅野明
  • Organizer: 園芸学会平成26年度秋季大会
  • Place of Presentation: 佐賀
  • Year and Date: 2014-09-27 – 2014-09-29
• [Presentation] ラン科シュンラン属の生活形･栄養摂取様式の進化－13: 独立栄養植物と菌従属栄養植物の雑種の世界初開花. (2014)
  • Author(s): 辻田有紀・三吉一光・堤千絵・遊川知久
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 川崎
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] ラン科シュンラン属の生活形･栄養、摂取様式の進化－12: 生活史ステージによる菌根菌シフトの種間比較. (2014)
  • Author(s): 木下晃彦・辻田有紀・遊川知久
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 川崎
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] 菌従属栄養植物タカツルランの菌根菌の多様性. (2014)
  • Author(s): 徐慧・辻田有紀・深澤遊・阿部晴恵・馬田英隆・手塚賢至・後藤利幸・牧雅之・遊川知久
  • Organizer: 日本菌学会第58回大会
  • Place of Presentation: 小松、石川県
  • Year and Date: 2014-06-13 – 2014-06-15
• [Presentation] 変化アサガオ「獅子系統」の腋芽培養における表現型の違いについて (2013)
  • Author(s): 古賀成彦・半田高
  • Organizer: 第31回植物細胞分子生物学会大会
  • Place of Presentation: 北海道大学
• [Book] ラン科植物の遺伝子解析最前線，リバティーアカデミーブックレット22巻「ランの世界」（夏井高人編） (2013)
  • Author(s): 半田 高 他
  • Total Pages: 46
  • Publisher: 明治大学リバティーアカデミー，東京