| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
To investigate mechanisms of occurance of necrosis and abnormal flowering of dormant buds in deciduous fruit tree, changes in mebobolome including a metabolome analysis, phytohormone analysis, bud burst and dormancy related gene expression analysis were performed using Japanese pear under different temperature conditions. From the results of these analysis, temperature fluctuation (TF) during both endodormancy and ecodormancy periods affected on many pathways of metabolism including sugar, organic acids, amino acids. Especially, pentose phosphate pathway was affected by TFs. In addition cyanamide treatment affected on beta-alanine, proline, glucose-6-phosphate. These results indicated that supply of energy source and oxidative stress would be factors invloved in dormancy breaking. Gene expresions of DAM, gene expresions related phytohormone synthesis and metabolism were chaned by TF treatments.
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