Metabolic regulation in dormant buds of deciduous fruit tree-Study of function of low temperature signal

• Project/Area Number: 25292019
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: University of Tsukuba
• Principal Investigator: SUGAYA Sumiko 筑波大学, 生命環境系, 教授 (90302372)
• Co-Investigator(Kenkyū-buntansha): 瀬古澤 由彦 筑波大学, 生命環境系, 助教 (90361310)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: 休眠 / ニホンナシ / メタボローム解析 / 遺伝子発現 / 植物ホルモン / 園芸学 / 果樹 / 代謝 / 落葉果樹 / 低温 / 糖代謝 / 果樹園芸学

Outline of Final Research Achievements

To investigate mechanisms of occurance of necrosis and abnormal flowering of dormant buds in deciduous fruit tree, changes in mebobolome including a metabolome analysis, phytohormone analysis, bud burst and dormancy related gene expression analysis were performed using Japanese pear under different temperature conditions. From the results of these analysis, temperature fluctuation (TF) during both endodormancy and ecodormancy periods affected on many pathways of metabolism including sugar, organic acids, amino acids. Especially, pentose phosphate pathway was affected by TFs. In addition cyanamide treatment affected on beta-alanine, proline, glucose-6-phosphate. These results indicated that supply of energy source and oxidative stress would be factors invloved in dormancy breaking. Gene expresions of DAM, gene expresions related phytohormone synthesis and metabolism were chaned by TF treatments.

Research Products

• [Journal Article] Metabolomics analysis of 'Housui' Japanese pear flower buds during endodormancy (2018)
  • Author(s): Horikoshi HM, Sekozawa Y, Kobayashi M, Saito K, Kusano M, Sugaya S.
  • Journal Title: Plant Physioly and Biochemistry Volume: 126 Pages: 134-141
  • DOI: 10.1016/j.plaphy.2018.02.028
  • Peer Reviewed
• [Journal Article] Inhibition of carbohydrate metabolism by thermal fluctuations during endodormancy lead to negative impacts on bud burst and incidence of floral necrosis in ‘Housui’ Japanese pear flower buds (2017)
  • Author(s): Horikoshi, HM, Sekozawa, Y., Sugaya, S.
  • Journal Title: Scientia horticulturae Volume: 1160 Pages: 221-226
  • DOI: 10.1016/j.scienta.2017.06.018
  • Peer Reviewed
• [Journal Article] Different thermal conditions during ecodormancy in Japanese pear affect floral bud necrosis, water and carbohydrate dynamics. (2017)
  • Author(s): Humberto Mitio Horikoshi, Yoshihiko Sekozawa, Sumiko Sugaya, Robson Ryu Yamamoto, Herter Gilberto Flavio
  • Journal Title: Acta Horticulturae Volume: 1160 Issue: 1160 Pages: 221-226
  • DOI: 10.17660/actahortic.2017.1160.32
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Metaboloc profiling of 'Hosui' Japanse pear flower buds treated with hydrogen cyanamide. (2017)
  • Author(s): Humberto HM. Y. Sekozawa, M. Kusano, S. Sugaya
  • Organizer: XIII International Symposium on Plant Bioregulators in Fruit Production
  • Int'l Joint Research