| Project/Area Number |
25292020
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Horticultural science
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| Research Institution | Ryukoku University (2016)
Kyoto University (2013-2015) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
神崎 真哉 近畿大学, 農学部, 准教授 (20330243)
佐藤 明彦 国立研究開発法人農業・食品産業技術総合研究機構, 果樹研究所, 上席研究員 (30355440)
山根 久代 京都大学, (連合)農学研究科(研究院), 講師 (80335306)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Keywords | 園芸学 / 果樹 / 甘渋性 / タンニン / 植物 / ゲノム |
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| Outline of Final Research Achievements |
The accuracy of AFLP markers linked to the locus of natural astringency-loss in Chinese-type PCNA persimmon were confirmed and then, the markers were used as probes for fosmid/BAC library constructed from Diospyros lotus, which is a close diploid relative of D. kaki, for constructing contig to identify the locus of natural astringency-loss. However, the results showed that the fosmid/BAC library is hard to use directly for identifying the locus probably due to the difference of genome structure between D. kaki and D. lotus. On the other hand, RNA-seq analysis using segregated progeny between Chinese-type PCNA and non-PCNA indicated that the genes related to transportation in the cell were differently expressed among them, as well as the genes related to flavonoid biosynthesis pathway.
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