Identification of genes controlling astringency in Chinese-type PCNA (pollination constant non-astringent) and its application to breeding for PCNA-type persimmon

• Project/Area Number: 25292020
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Ryukoku University (2016); Kyoto University (2013-2015)
• Principal Investigator: Yonemori Keizo 龍谷大学, 農学部, 教授 (10111949)
• Co-Investigator(Kenkyū-buntansha): 神崎 真哉 近畿大学, 農学部, 准教授 (20330243) ; 佐藤 明彦 国立研究開発法人農業・食品産業技術総合研究機構, 果樹研究所, 上席研究員 (30355440) ; 山根 久代 京都大学, (連合)農学研究科(研究院), 講師 (80335306)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
• Keywords: 園芸学 / 果樹 / 甘渋性 / タンニン / 植物 / ゲノム

Outline of Final Research Achievements

The accuracy of AFLP markers linked to the locus of natural astringency-loss in Chinese-type PCNA persimmon were confirmed and then, the markers were used as probes for fosmid/BAC library constructed from Diospyros lotus, which is a close diploid relative of D. kaki, for constructing contig to identify the locus of natural astringency-loss. However, the results showed that the fosmid/BAC library is hard to use directly for identifying the locus probably due to the difference of genome structure between D. kaki and D. lotus. On the other hand, RNA-seq analysis using segregated progeny between Chinese-type PCNA and non-PCNA indicated that the genes related to transportation in the cell were differently expressed among them, as well as the genes related to flavonoid biosynthesis pathway.

Research Products

• [Journal Article] Application of marker-assisted selection in persimmon breeding of PCNA offspring using SCAR markers among the population from the cross between non-PCNA 'Taigetsu' and PCNA 'Kanshu' (2014)
  • Author(s): Mitani, N., A. Kono, M. Yamada, A. Sato, S. Kobayashi, Y. Ban, T. Ueno, M. Shiraishi, S. Kanzaki, T. Tsujimoto, K. Yonemori
  • Journal Title: HortScience Volume: 49 Pages: 1132-1135
  • Peer Reviewed
• [Presentation] Toward Isolation of a Gene Conferring Astringency Loss in Persimmon Fruit (2017)
  • Author(s): Nishiyama, S., N. Onoue, A. Kono, A. Sato2, K. Yonemori and R. Tao
  • Organizer: PLANT AND ANIMAL GENOME XXIV
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 2017-01-14
  • Int'l Joint Research
• [Presentation] Differential expression analysis of the genes conferring non-astringent trait at the two different loci in Japanese- and Chinese-type pollination constant non-astringent (PCNA) persimmon (2016)
  • Author(s): Yonemori, K., S. Nishiyama, A. Kono, N. Onoue and A. Sato
  • Organizer: VI International Symposium on Persimmon
  • Place of Presentation: VALENCIA, SPAIN
  • Year and Date: 2016-10-17
  • Int'l Joint Research
• [Presentation] カキ甘渋性に関するトランスクリプトーム解析 (2016)
  • Author(s): 西山総一郎・尾上典之・河野 淳・佐藤明彦・米森敬三・田尾龍太郎
  • Organizer: 園芸学会平成28年度秋季大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2016-09-10
• [Presentation] Comparative genomics of the locus controlling astringency in Diospyros species (2016)
  • Author(s): Nishiyama, S., A. Kono, N. Onoue, A. Sato, K. Ushijima, H. Yamane, R. Tao and K. Yonemori
  • Organizer: Plant and Animal Genome XXIV
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 2016-01-09
  • Int'l Joint Research