| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
DREB1A encoding the transcription factor that improves abiotic stress tolerance of plants. There are many DREB1A gene orthologs in various plant species. However, in lettuce (Lactuca sativa L.), single nucleotide polymorphism (SNP) is exist at 70th tryptophane to stop codon. In this study, the relationship between the SNP and stress tolerance was verified by investigating overexpressed transformants and F2 generations.
Under freezing, drought and salt stress condition, transgenic lettuce overexpressing LsDREB1A-X70W lines showed the higher survival rate. This phenomenon was supported by stress-related target genes revealed by RNA-seq analysis. Both X70W-genotype and heterozygous F2 showed a significant higher survival rate as compared to W70X-genotype. These results suggest that the SNP of LsDREB1A is utilized as DNA marker in environmental stress tolerance of lettuce, and the cultivars possessing wild-type genotype X70W could be used as a useful breeding material.
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