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Molecular Analysis for heterostyly by using genomics and transcriptmics approches

Research Project

Project/Area Number 25292023
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Horticultural science
Research InstitutionOkayama University

Principal Investigator

Ushijima Koichiro  岡山大学, 大学院環境生命科学研究科, 准教授 (20379720)

Co-Investigator(Kenkyū-buntansha) 鈴木 孝征  中部大学, 公私立大学の部局等, 講師 (50535797)
赤木 剛士  京都大学, (連合)農学研究科(研究院), 助教 (50611919)
池田 和生  山形大学, 農学部, 准教授 (80555269)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Keywords異形花型自家不和合性 / de novo assembly / S遺伝子座 / 自家不和合性 / トランスクリプトーム / PacBio / 次世代シークエンサー
Outline of Final Research Achievements

Heterostyly is one of self-incompatibility that is a genetic system to reject the self-pollen and be linked to floral morph polymorphism.In this study, to elucidate the molecular mechanism of Linum heterostyly, we isolated the candidate gene controlling heterostyly and characterized the S locus region. Four candidate gene were newly isolated by transcriptome analysis. Genome analysis revealed the S haplotype specific region at least reached more that 1 Mbp. All candidate genes were included in this region but the relative locations were unclear. We further shotgun sequenced the genome of Linum grandiflorum and de novo assembly. The assembled contigs will give new insights on the study of heterostyly.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (9 results)

All 2016 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Invited: 1 results)

  • [Journal Article] Genetic Control of Floral Morph and Petal Pigmentation in <i>Linum grandiflorum</i> Desf., a Heterostylous Flax2015

    • Author(s)
      Koichiro Ushijima, Kazuo Ikeda, Ryohei Nakano, Miyo Matsubara, Yuri Tsuda, Yasutaka Kubo
    • Journal Title

      The Horticulture Journal

      Volume: 84 Issue: 3 Pages: 261-268

    • DOI

      10.2503/hortj.MI-045

    • NAID

      130005088754

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] アマ科植物の異形花型自家不和合性遺伝子座領域に座乗する遺伝子の同定2016

    • Author(s)
      牛島幸一郎, 赤木剛士, 池田和生, 七條由衣, 島田浩平, 中野龍平, 久保 康隆
    • Organizer
      育種学会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] NGS大量リードデータのサブリード比較によるアマ科植物の異形花型自家不和合性遺伝子座の解析2016

    • Author(s)
      牛島幸一郎, 赤木剛士, 池田和生, 七條由衣, 島田浩平, 中野龍平, 久保 康隆
    • Organizer
      園芸学会
    • Place of Presentation
      名城大学
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] 異形花型自家不和合性の制御因子の探索2015

    • Author(s)
      牛島幸一郎
    • Organizer
      日本育種学会中四国談話会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-12-19
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 大量シークエンスデータのサブリード比較によるアマ科異形花型自家不和合性関連遺伝子の単離2015

    • Author(s)
      牛島幸一郎、赤木剛士、池田和生、七條由衣、中野龍平、久保康隆
    • Organizer
      園芸学会
    • Place of Presentation
      徳島大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 次世代シークエンスプールのkmer比較を利用したアマ科異形花不和合性の花粉側候補遺伝子の探索2015

    • Author(s)
      牛島幸一郎、赤木剛士、池田和生、中野龍平、久保康隆
    • Organizer
      日本育種学会
    • Place of Presentation
      新潟大学
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] アマ科異形花型自家不和合性植物の花器官の形態的特徴と遺伝様式2014

    • Author(s)
      牛島幸一郎・池田和生・松原未葉・久保康隆・中野龍平
    • Organizer
      園芸学会中四国支部会
    • Place of Presentation
      徳島県立農林水産総合技術支援センター
    • Year and Date
      2014-07-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] アマ科植物の異形花型自家 不和合性の解析 VI. de novo assembly 解析による花型特異 的発現遺伝子の同定2013

    • Author(s)
      牛島 幸一郎・鈴木 孝征・中野 龍平・東山 哲也・久保 康隆
    • Organizer
      園芸学会H25年度秋季大会
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ベニバナアマにおける異形花型自家不和合性の解 析 V : 次世代シークエンサーを用いた新規 S 遺伝 子候補の探索2013

    • Author(s)
      牛島 幸一郎・鈴木 孝征・中野 龍平・東山 哲也・久保 康隆
    • Organizer
      日本育種学会 第124回講演会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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