| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Outline of Final Research Achievements |
We have searched for transcription factor(s) that bind to the promoter region of chlorophyll metabolic genes using a library composed only of transcription factor cDNAs of Arabidopsis. Among transcription factors identified by the screening, we focused on ANAC046 that specifically bound to the promoters of chlorophyll catabolic genes including SGR1, SGR2, NYC1 and PaO. Analysis of mutant and transgenic ANAC046 plants showed that ANAC046 is a positive regulator of leaf senescence and exerts its effect by controlling the expression of Chl catabolic genes and senescence-associated genes. Transgenic petunia plants introduced ANAC046-SRDX construct showed a marked increase in chlorophyll content in their flowers, indicating that ANAC046 function as a positive regulator of chlorophyll degradation in petunia plants.
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