| Budget Amount *help |
¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Outline of Final Research Achievements |
To gain insight into the reduction of a Japanese pear DAM homolog (PpMADS13-1) expression toward endodormancy release, we investigated the histone modification in PpMADS13-1 locus via chromatin immunoprecipitation-quantitative PCR. Our results indicated that reduction in the active histone mark by trimethylation of the histone H3 tail at lysine 4 and loss of histone variant H2A.Z contributed to the reduction of PpMADS13-1 expression toward endodormancy release. Subsequently, we found that PpCBF2, a pear C-repeated binding factor, regulated PpMADS13-1 expression via interaction of PpCBF2 with the 5′-upstream region of PpMADS13-1. Furthermore, transient reporter assay confirmed no interaction between the PpMADS13-1 protein and the pear FLOWERING LOCUS T genes. We also showed that the size of flower buds did not change significantly during endodormancy, but rapid enlargement took place at the end of ecodormancy concomitant with the increase in free water content.
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