| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
We performed genetic analyses of eight putative histone lysine methyltransferase (KMT) genes in the phytopathogenic fungus Pyricularia oryzae. Phenotypic assays revealed that MoSET1 disruptants (Δmoset1) showed the most severe defects in infection-related morphogenesis, including conidiation and appressorium formation, indicating that H3K4me is an important epigenetic mark for infection-related gene expression in P. oryzae. ChIP-seq analysis of H3K4 di- and tri-methylation (H3K4me2/me3) and MoSET1 protein during infection-related morphogenesis, together with RNA-seq analysis of the Δmoset1 mutant, led to the following conclusions: 1) In general, H3K4-me2 and -me3 abundance was positively associated with active transcription. 2) Among the 4,077 differentially expressed genes (DEGs) between mycelia and germination tubes, 1,201 and 882 genes were up- and down-regulated, respectively, in a MoSET1-dependent manner.
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