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Epigenetic gene regulation of the rice blast fungus Pyricularia oryzae during infection

Research Project

Project/Area Number 25292028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant protection science
Research InstitutionKobe University

Principal Investigator

NAKAYASHIKI Hitoshi  神戸大学, (連合)農学研究科(研究院), 教授 (50252804)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywordsいもち病菌 / エピジェネティクス / ヒストン修飾 / 病原性 / 植物保護 / ヒストンメチル化
Outline of Final Research Achievements

We performed genetic analyses of eight putative histone lysine methyltransferase (KMT) genes in the phytopathogenic fungus Pyricularia oryzae. Phenotypic assays revealed that MoSET1 disruptants (Δmoset1) showed the most severe defects in infection-related morphogenesis, including conidiation and appressorium formation, indicating that H3K4me is an important epigenetic mark for infection-related gene expression in P. oryzae. ChIP-seq analysis of H3K4 di- and tri-methylation (H3K4me2/me3) and MoSET1 protein during infection-related morphogenesis, together with RNA-seq analysis of the Δmoset1 mutant, led to the following conclusions: 1) In general, H3K4-me2 and -me3 abundance was positively associated with active transcription. 2) Among the 4,077 differentially expressed genes (DEGs) between mycelia and germination tubes, 1,201 and 882 genes were up- and down-regulated, respectively, in a MoSET1-dependent manner.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (11 results)

All 2015 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (6 results) (of which Invited: 3 results) Remarks (1 results)

  • [Journal Article] MoSET1 (Histone H3K4 Methyltransferase in Magnaporthe oryzae) Regulates Global Gene Expression during Infection-Related Morphogenesis.2015

    • Author(s)
      Pham KT, Inoue Y, Vu BV, Nguyen HH, Nakayashiki T, Ikeda K, Nakayashiki H.
    • Journal Title

      PLOS Genetics

      Volume: 11 Issue: 7 Pages: e1005385-e1005385

    • DOI

      10.1371/journal.pgen.1005385

    • NAID

      120005745418

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Histone H3K4 methyltransferase globally regulates substrate-dependent activation of cell wall degradation enzymes in Magnaporthe oryzae2015

    • Author(s)
      Pham, TMK ; Nguyen, H.H. ; Murai,T et al.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 81 Issue: 2 Pages: 127-130

    • DOI

      10.1007/s10327-014-0570-y

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Substrate-induced transcriptional activation of the MoCel7C cellulase gene is associated with methylation of histone H3 at lysine 4 in the rice blast fungus Magnaporthe oryzae.2013

    • Author(s)
      Vu BV, Pham KT, Nakayashiki H.
    • Journal Title

      Appl Environ Microbiol

      Volume: 79 Issue: 21 Pages: 6823-6832

    • DOI

      10.1128/aem.02082-13

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Is the fungus Magnaporthe losing DNA methylation?2013

    • Author(s)
      Ikeda, K., Vu, B. V., Kadotani, N., Tanaka, M., Murata, T., Shiina, K., Chuma, I., Tosa, Y., and Nakayashiki H
    • Journal Title

      Genetics

      Volume: 195 Issue: 3 Pages: 845-855

    • DOI

      10.1534/genetics.113.155978

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] いもち病菌ゲノムのエピジェネティクな遺伝子制御2015

    • Author(s)
      Pham,T.M.K., Inoue, Y., Vu, B.V., Ikeda, K., Nakayashiki, H.
    • Organizer
      H27年度植物感染生理談話会
    • Place of Presentation
      メルパルク松山(愛媛県・松山市)
    • Year and Date
      2015-08-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] MAPキナーゼPmk1およびMps1はH3K4ヒストンメチル基転移酵素MoSET1の上流で機能する2015

    • Author(s)
      Nguyen Hieu Hanh, Kieu Thi Minh Pham, 池田健一, 中屋敷均
    • Organizer
      日本植物病理学会
    • Place of Presentation
      明治大学 東京
    • Year and Date
      2015-03-29 – 2015-03-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Histone methyltransferase genes play roles in the pathogenicity of the rice blast fungus, Magnaporthe oryzae.2013

    • Author(s)
      Nakayashiki, H
    • Organizer
      Sino-Japan Joint Symposium of Plant Pathology
    • Place of Presentation
      中国、北京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Histone lysine methyltransferases in Magnaporthe oryzae are involved in various aspects of infection and pathogenesis.2013

    • Author(s)
      Pham, K. T. M., Vu, B. V., Nguyen, Q. B., Ikeda, K., and Nakayashiki, H.
    • Organizer
      10th International Congress of Plant Pathology
    • Place of Presentation
      中国、北京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] MoSET1, histone methyltransferase responsible for methylation of histone H3 lysine 4 in Magnaporthe oryzae is required for both activation of gene expression during infection-related morphogenesis of the fungus.2013

    • Author(s)
      Pham, K. T. M., Vu, B. V., Ikeda, K., and Nakayashiki, H.
    • Organizer
      平成25年度日本植物病理学会 関西部会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] いもち病菌におけるヒストンリジンメチルトランスフェラーゼの役割2013

    • Author(s)
      Pham, K. T. M., 井上喜博, Vu, B. V., Nguyen, Q. B., 池田健一、中屋敷均
    • Organizer
      第13回糸状菌分子生物学コンファレンス
    • Place of Presentation
      つくば市
    • Related Report
      2013 Annual Research Report
  • [Remarks] 細胞機能構造学コース

    • URL

      http://www.ans.kobe-u.ac.jp/jyukensei/e-bio/guide/structure.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

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