Epigenetic gene regulation of the rice blast fungus Pyricularia oryzae during infection
Project/Area Number |
25292028
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Plant protection science
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Research Institution | Kobe University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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Keywords | いもち病菌 / エピジェネティクス / ヒストン修飾 / 病原性 / 植物保護 / ヒストンメチル化 |
Outline of Final Research Achievements |
We performed genetic analyses of eight putative histone lysine methyltransferase (KMT) genes in the phytopathogenic fungus Pyricularia oryzae. Phenotypic assays revealed that MoSET1 disruptants (Δmoset1) showed the most severe defects in infection-related morphogenesis, including conidiation and appressorium formation, indicating that H3K4me is an important epigenetic mark for infection-related gene expression in P. oryzae. ChIP-seq analysis of H3K4 di- and tri-methylation (H3K4me2/me3) and MoSET1 protein during infection-related morphogenesis, together with RNA-seq analysis of the Δmoset1 mutant, led to the following conclusions: 1) In general, H3K4-me2 and -me3 abundance was positively associated with active transcription. 2) Among the 4,077 differentially expressed genes (DEGs) between mycelia and germination tubes, 1,201 and 882 genes were up- and down-regulated, respectively, in a MoSET1-dependent manner.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Is the fungus Magnaporthe losing DNA methylation?2013
Author(s)
Ikeda, K., Vu, B. V., Kadotani, N., Tanaka, M., Murata, T., Shiina, K., Chuma, I., Tosa, Y., and Nakayashiki H
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Journal Title
Genetics
Volume: 195
Issue: 3
Pages: 845-855
DOI
Related Report
Peer Reviewed
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[Presentation] いもち病菌ゲノムのエピジェネティクな遺伝子制御2015
Author(s)
Pham,T.M.K., Inoue, Y., Vu, B.V., Ikeda, K., Nakayashiki, H.
Organizer
H27年度植物感染生理談話会
Place of Presentation
メルパルク松山(愛媛県・松山市)
Year and Date
2015-08-24
Related Report
Invited
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