Induction mechanisms of susceptibility to bacterial wilt and development of its monitoring system
Project/Area Number |
25292029
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Plant protection science
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Research Institution | Kochi University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
大西 浩平 高知大学, その他の研究科, 教授 (50211800)
木場 章範 高知大学, その他の研究科, 教授 (50343314)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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Keywords | 植物-病原体相互作用 / 植物免疫 / 感受性誘導 / 植物細菌病 / エフェクター / 青枯病 / 感受性 |
Outline of Final Research Achievements |
We developed the model system for evasion of Ralstonia solanacearum-induced innate immunity by Nicotiana benthamiana and elucidate jasmonic acid-mediated innate immunity used phosphatidic acid as a substrate. It was required for several effectors secreted by R. solanacearum to induce expression of phosphatidic acid phosphatase gene in N. benthamiana, evading its innate immunity. This innate immunity was induced by infection with other plant bacteria such as Pseudomonas syringae. These bacteria also secrete effectors through the type III secretion machinery to induce expression of phosphatidic acid phosphatase gene, evading innate immunity by N. benthamiana. After evading innate immunity, virulent strain OE1-1 of R. solanacearum grew on host cells and produced mushroom-type biofilms which was required for its virulence. Taken together, these results open the door to uncover key interactions between host plants and R. solanacearum at the initial stage of infection.
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Report
(5 results)
Research Products
(92 results)
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[Journal Article] Involvement of ralfuranones in the quorum sensing signaling pathway and virulence of Ralstonia solanacearum strain OE1-12017
Author(s)
Mori, M., Ishikawa, S., Ohnishi, H., Shimatani, M., Morikawa, Y., Hayashi, K., Ohnishi, K., Kiba, A., Kai K. and Hikichi, Y.
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Journal Title
Molecular Plant Pathology
Volume: 印刷中
Issue: 2
Pages: 454-463
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Silencing of <i>Nicotiana benthamiana</i> SEC14 phospholipid transfer protein reduced jasmonic acid dependent defense against <i>Pseudomonas syringae</i>2016
Author(s)
Kiba, A., Imanaka, Y., Nakano, M., Galis, I., Hojo, Y., Shinya, T., Ohnoshi, K. and Hikichi, Y.
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Journal Title
Plant Biotechnology
Volume: 33
Issue: 2
Pages: 111-115
DOI
NAID
ISSN
1342-4580, 1347-6114
Related Report
Peer Reviewed
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[Journal Article] SEC14 phospholipid transfer protein is involved in lipid signaling-mediated plant immune responses in Nicotiana benthamiana.2014
Author(s)
Kiba, A., Galis, I., Hojo, Y., Ohnishi, K., Yoshioka, H., Hikichi, Y.,
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Journal Title
Plos One
Volume: 9
Issue: 5
Pages: e98150-e98150
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Heat shock protein 70 is required for tabtoxinine- B-lactam-induced cell death in Nicotiana benthamiana2014
Author(s)
Ito, M., Yamamoto, Y., Kim, C-S., Ohnishi, K., Hikichia, Y., Kiba, A.
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Journal Title
Journal of Plant Physiology
Volume: 171
Issue: 2
Pages: 173-178
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Heat shock protein 70 is required for tabtoxinine- β-lactam-induced cell death in Nicotiana benthamiana.2013
Author(s)
Ito, M., Yamamotob, Y., Kim, C-S., Ohnishi, K., Hikichia, Y., Kiba, A.
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Journal Title
Journal of Plant Physiology
Volume: 171
Pages: 173-821
Related Report
Peer Reviewed
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[Journal Article] Suppression of DS1 phosphatidic acid phosphatase confirms resistance to Ralstonia solanacearum in Nicotiana benthamiana2013
Author(s)
Nakano, M., Nishihara, M., Yoshioka, H., Takahashi, H., Sawasaki, T., Ohnishi, K., Hikichi, Y., and Kiba, A.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Involvement of HLK effectors in Ralstonia solanacearum disease development in tomato2013
Author(s)
Chen, L., Shirota, M., Zhang, Y., Kiba, A., Hikichi, Y., and Ohnishi, K.
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Journal Title
Journal of General Plant Pathology
Volume: 80
Pages: 79-84
Related Report
Peer Reviewed
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[Presentation] 細胞間隙へ侵入後の青枯病菌OE1-1 株によるバイオフィルム形成は,methyl 3-hydroxymyristate をシグナルとするクオラムセンシングに依存しており,病原性に必須である2015
Author(s)
曵地康史, 森友花, 石川詩歩, 東本周樹, 大西秀幸, 嶋谷美香, 井上加奈子, 池田健一, 中屋敷均, 木場章範, 大西浩平, 甲斐建次
Organizer
植物微生物研究会第25回研究交流会
Place of Presentation
つくば国際会議場、つくば市
Year and Date
2015-09-14
Related Report
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[Presentation] Biofilm formation of Ralstonia solanacaerum in intercellular spaces and implication of ralfuranones in its biofilm formation2014
Author(s)
Yuka Mori, Hideyuki Ohnishi, KanakoInoue, Kenichi Ikeda, Hitoshi Nakayashiki, Shiho Ishikawa, AkinoriKiba, KouheiOhnishi, Kenji Kai, YasufumiHikichi
Organizer
The 13th Internationals Conference on Plant Pathogenic Bacteria
Place of Presentation
Shanghai Everbright International Hotel (Shanghai, China)
Year and Date
2014-06-08 – 2014-06-13
Related Report
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