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Analyses of differentially expressed genes in Rosellinia necatrix infected with the mycovirus that decreases host fungus virulence

Research Project

Project/Area Number 25292031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant protection science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Kanematsu Satoko  国立研究開発法人農業・食品産業技術総合研究機構, 本部人事部, 室長 (40355433)

Co-Investigator(Kenkyū-buntansha) 八重樫 元  国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門 リンゴ研究領域, 主任研究員 (90582594)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords白紋羽病 / 糸状菌病 / マイコウイルス / 病原力低下 / 植物病原糸状菌 / 植物病理学 / 白紋羽病菌 / 病原性
Outline of Final Research Achievements

Rosellinia necatrix is the causal fungus of white root rot disease of fruit trees. To elucidate the molecular mechanisms underlying the pathogenicity of this fungus, the debilitated W97 strain infected with Rosellinia necatrix megabirnavirus 1 (RnMBV1) underwent a comprehensive transcriptional analysis. Among the differentially expressed genes, 10 were functionally analyzed based on RNAi-mediated gene silencing or overexpression. None of the genes affected the virulence of R. necatrix, but the melanin biosynthesistic gene contributed to the long-time survival of this fungus in soil via morphogenesis.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (16 results)

All 2017 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 2 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Differential inductions of RNA silencing among encapsidated double-stranded RNA mycoviruses in the white root rot fungus, Rosellinia necatrix.2016

    • Author(s)
      Hajime Yaegashi, Takeo Shimizu, Tsutae Ito, Satoko Kanematsu.
    • Journal Title

      Journal of Virology

      Volume: * Issue: 12 Pages: 5677-5692

    • DOI

      10.1128/jvi.02951-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Systemic RNA interference is not triggered by locally-induced RNA interference in a plant pathogenic fungus, Rosellinia necatrix2015

    • Author(s)
      Takeo Shimizu, Hajime Yaegashi, Tsutae Ito, Satoko Kanematsu
    • Journal Title

      Fungal Genetics and Biology

      Volume: 76 Pages: 27-35

    • DOI

      10.1016/j.fgb.2015.02.001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Functional analysis of a melanin biosynthetic gene using RNAi-mediated gene silencing in Rosellinia necatrix2014

    • Author(s)
      Takeo SHIMIZU, Tsutae ITO, Satoko KANEMATSU
    • Journal Title

      Fungal Biology

      Volume: 118 Issue: 4 Pages: 413-421

    • DOI

      10.1016/j.funbio.2014.02.006

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome rearrangement of a mycovirus Rosellinia necatrix megabirnavirus 1 affecting its ability to attenuate virulence of the host fungus.2014

    • Author(s)
      Kanematsu S, Shimizu T, Salaipeth L, Yaegashi H, Sasaki A, Ito T, Suzuki N
    • Journal Title

      Virology

      Volume: 450-451 Pages: 308-315

    • DOI

      10.1016/j.virol.2013.12.002

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 果樹白紋羽病菌に対するマイコウイルスの感染・移行戦略2017

    • Author(s)
      八重樫 元
    • Organizer
      第17回植物病原菌類談話会
    • Place of Presentation
      アイーナ (岩手県盛岡市)
    • Year and Date
      2017-04-28
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 果樹の病原糸状菌を病気にするウイルス2017

    • Author(s)
      八重樫 元
    • Organizer
      園芸学会平成29年度春季大会小集会「園芸学とウイルス学の異分野融合研究会」
    • Place of Presentation
      日本大学生物資源科学部 (神奈川県藤沢市)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] メガビルナウイルスは白紋羽病菌においてRNA誘導サイレンシング複合体を活性化させる2016

    • Author(s)
      八重樫元、清水健雄、伊藤伝、兼松聡子
    • Organizer
      平成28年度日本植物病理学会
    • Place of Presentation
      岡山コンベンションセンター
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mycoviruses in Rosellinia necatrix: Features of potential virocontrol agents2015

    • Author(s)
      Satoko Kanematsu, Hajime Yaegashi
    • Organizer
      23rd International conference on virus and other graft transmissible diseases of fruit crops
    • Place of Presentation
      岩手県盛岡市アイーナセンター
    • Year and Date
      2015-06-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 各種培養条件における白紋羽病菌の性状2014

    • Author(s)
      清水健雄、伊藤伝、兼松聡子
    • Organizer
      平成26年度 日本植物病理学会東北部会
    • Place of Presentation
      盛岡市アイーナ
    • Year and Date
      2014-09-25 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Suppression of RNA silencing by mycoreovirus in the white root rot fungus, Rosellinia necatrix2014

    • Author(s)
      Yaegashi, H., Shimizu, T., Ito, T., Kanematsu, S.
    • Organizer
      3rd International Mycovirus Symposium
    • Place of Presentation
      The university of Vermont
    • Year and Date
      2014-08-02 – 2014-08-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Functional analysis of a melanin biosynthetic gene using RNAi-mediated gene silencing in Rosellinia necatrix2014

    • Author(s)
      Kanematsu, S., Shimizu, T., Ito, T.
    • Organizer
      3rd International Mycovirus Symposium
    • Place of Presentation
      The university of Vermont
    • Year and Date
      2014-08-02 – 2014-08-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Virulent mycoviruses up-regulate RNA silencing related genes in a phytopathogenic fungus, Rosellinia necatrix2014

    • Author(s)
      Yaegashi, H., Shimizu, T., Ito, T., Kanematsu, S.
    • Organizer
      The 16th International Congress of Virology
    • Place of Presentation
      Montreal convention center
    • Year and Date
      2014-07-26 – 2014-08-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] マイコウイルス感染による白紋羽病菌RNAサイレンシング関連遺伝子の発現上昇2014

    • Author(s)
      八重樫元、清水健雄、伊藤伝、兼松聡子
    • Organizer
      平成26年度 日本植物病理学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] 白紋羽病菌Rosellinia necatrix のメガビルナウイルス感染株におけるトランスクリプトーム解析2014

    • Author(s)
      清水健雄、八重樫元、伊藤伝、中屋敷均、兼松聡子
    • Organizer
      平成26年度 日本植物病理学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rosellinia necatrix megabirnavirus 1 の機能未知ORFの部分欠損変異株の解析2014

    • Author(s)
      兼松聡子、清水健雄、八重樫元、伊藤伝、
    • Organizer
      平成26年度 日本植物病理学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Annual Research Report
  • [Book] Transformation of ascomycetous fungi using autonomously replicating fungi, in Genetic Transformation Systems in fungi, Vol.22014

    • Author(s)
      Shimizu, T., Kanematsu, S.
    • Total Pages
      270
    • Publisher
      Springer International Publishing
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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