| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
Molecular information of spider mites is increasingly used to answer taxonomic and systematic questions. The COI gene and ITS2 region of nuclear ribosomal RNA have been used for phylogenetic reconstruction within the Tetranychidae, but these sequences have not consistently resolved genus-level phylogenetic relationships, because of low bootstrap values. So, we performed phylogenomics analysis for spider mites, derived from comparative RNA-Seq data for 88 strains of 87 species belonging to 15 genera. A phylogenetic tree using 652 orthologous protein-coding genes shared among taxa, was well-resolved and strongly supported for almost all nodes (67/70), allowing us to consider the phylogenetic relationships among genera of Tetranychidae. The topology presented here does not fully agree with the current taxonomic treatment, because the four genera were polyphyletic. These results indicate that the diagnostic morphological characters of the genera of Tetranychidae must be reconsidered.
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