Study for gene expression control mechanism involved in the soil environment adaptation strategies of actinomycetes
| Project/Area Number |
25292040
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | National Institute for Agro-Environmental Sciences |
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Principal Investigator |
FUJII Takeshi 国立研究開発法人 農業環境技術研究所, その他部局等, 領域長 (00354100)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Akihiro 静岡理工科大学, 理工学部, 准教授 (50375614)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | 遺伝子発現制御 / 転写カスケード / キチナーゼ / 抗生物質生産 / 放線菌 / 転写因子 |
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| Outline of Final Research Achievements |
In Streptomyces coelicolor growing in soil amended with chitin, a transcription factor DasR is involved in the regulation of expression of chitin metabolism-related genes and antibiotic production genes. In this study, in order to clarify the metabolic regulation cascade around the DasR, we tried to find factors which regulate dasR expression. As a result, we found that DasR itself binds strongly to dasR promoter, also that a transcription factor whose deletion leads to over production of chitinase and loss of catabolite repression of chitinase production controls expression of dasR.
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Report
(4 results)
Research Products
(16 results)
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[Presentation] Uptake of a chitin-degradation product via the constitutive N-acetylglucosamine/N,N’-diacetylchitobiose binding protein NgcESCO of an ABC transporter in Streptomyces coelicolor A3(2)2015
Author(s)
Akihiro Saito, Chibaru Iinuma, Takayuki Ohnuma, Tomonori Shinya, Yusuke Kimura, Yuzusa Aoki, Liu Feng, Yoshitake Desaki, Naoto Shibuya, Tamo Fukamizo, Akikazu Ando, Takeshi Fujii, Kiyotaka Miyashita
Organizer
日本微生物生態学会
Place of Presentation
土浦亀城プラザ
Year and Date
2015-10-19
Related Report
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