Mechanisms for acquiring ability to degrade novel compounds in sphingomonads and their applications
Project/Area Number |
25292043
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
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Research Institution | Tohoku University |
Principal Investigator |
Nagata Yuji 東北大学, 生命科学研究科, 准教授 (30237531)
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Co-Investigator(Kenkyū-buntansha) |
TSUDA MASATAKA 東北大学, 大学院生命科学研究科, 教授 (90172022)
OHTSUBO YOSHIYUKI 東北大学, 大学院生命科学研究科, 助教 (40342761)
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Research Collaborator |
Jiri Damborsky マサリク大学, 理学部, 教授
Zbynek Prokop マサリク大学, 理学部, 准教授
TANOKURA MASARU 東京大学, 大学院農学生命科学研究科, 教授 (60136786)
OKAI MASAHIKO 東京海洋大学, 大学院海洋科学技術研究科, 助教 (00596562)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
|
Keywords | スフィンゴモナス / 環境汚染物質 / 可動性遺伝因子 / ゲノム / 進化 |
Outline of Final Research Achievements |
Studies on degradation of a recalcitrant environmental pollutant, gamma-hexachlorocyclohexane, in sphingomonads revealed that (i) activity of a key enzyme was improved in stepwise manner by the accumulation of single mutations, (ii) sphingomands-specific plasmids and an insertion sequence play significant roles to acquire novel genes, (iii) sphingomonads have genetic background suitable for the degradation of recalcitrant compounds. These important knowledges for the functional evolution of bacteria in the environment can be used for the practical applications in the future.
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Report
(4 results)
Research Products
(103 results)
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[Journal Article] Complete Genome sequence of a gamma-hexachlorocyclohexane degrader, Sphingobium sp. Strain TKS, which was isolated from a gamma-hexachlorocyclohexane-degrading microbial community2016
Author(s)
Tabata M, Ohhata S, Kawasumi T, Nikawadori Y, Kishida K, Sato T, Ohtsubo Y, Tsuda M, Nagata Y
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Journal Title
Genome Announcements
Volume: 4
Issue: 2
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Complete genome sequence of a phenanthrene degrader, Mycobacterium sp. strain EPa45 (NBRC 110737), isolated from a phenanthrene-degrading consortium.2015
Author(s)
Kato H, Ogawa N, Ohtsubo Y, Oshima K, Toyoda A, Mori H, Nagata Y, Kurokawa K, Hattori M, Fujiyama A, Tsuda M
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Journal Title
Genome Announcements
Volume: 3
Issue: 4
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Time-series metagenomic analysis reveals robustness of soil microbiome against chemical disturbance.2015
Author(s)
Kato H, Mori H, Maruyama F, Toyoda A, Oshima K, Endo R, Fuchu G, Miyakoshi M, Dozono A, Ohtsubo Y, Nagata Y, Hattori M, Fujiyama A, Kurokawa K, Tsuda M
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Journal Title
DNA Research
Volume: 22
Issue: 6
Pages: 413-424
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Complete genome sequence of Sphingompyxis macrogoltabidus type strain NBRC 15033, originally isolated as a polyethylene glycol-degrader2015
Author(s)
Ohtsubo Y, Nagata, Y, Numata M, Tsuchikane K, Hosoyama A, Yamazoe A, Tsuda M, Fujita N, Kawai F
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Journal Title
Genome Announcements
Volume: 3
Issue: 6
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Structural and Functional Analysis of Novel Haloalkane Dehalogenase with Two Halide-Binding Sites2014
Author(s)
Chaloupkova R, Prudnikova T, Rezacova P, Prokop Z, Koudelakova T, Daniel L, Brezovsky J, Ikeda-Ohtsubo W, Sato Y, Kuty M, Nagata Y, Smatanova IK, Damborsky J
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Journal Title
Acta Crystallographica Section D
Volume: D70
Issue: 7
Pages: 1884-1897
DOI
Related Report
Peer Reviewed
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[Journal Article] Complete genome sequence of the thermophilic PCB degrader Geobacillus sp. JF8 (NBRC 109937)2014
Author(s)
Shintani, M., Y. Ohtsubo, K. Fukuda, A. Hosoyama, S. Ohji, A. Yamazoe, N. Fujita, Y. Nagata, M. Tsuda, T. Hatta, and K. Kimbara
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Journal Title
Genome Announcements
Volume: 2
Issue: 1
DOI
Related Report
Peer Reviewed
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[Presentation] Haloalkane dehalogenases in bacteria2015
Author(s)
Yuji Nagata, Ryota Moriuchi, Yoshiyuki Ohtsubo, and Masataka Tsuda
Organizer
The International Chemical Congress of Pacific Basin Societies (Pacifichem 2015)
Place of Presentation
Honolulu, Hawaii, USA
Year and Date
2015-12-15
Related Report
Int'l Joint Research / Invited
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[Presentation] リボソームRNA遺伝子の染色体外への移行2015
Author(s)
按田瑞恵, 大坪嘉行, 大久保卓, 菅原雅之, 永田裕二, 津田雅孝, 南澤究, 三井久幸
Organizer
日本遺伝学会第87回大会ワークショップ12「ゲノムとOMICSの比較から現れた遺伝と進化をめぐる驚き」
Place of Presentation
東北大学川内北キャンパス(宮城県、仙台市)
Year and Date
2015-09-24
Related Report
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[Presentation] 土壌微生物の移植メタゲノム2014
Author(s)
加藤広海, 森宙史, 丸山史人, 豊田敦, 永山浩史, 大坪嘉行, 永田裕二, 藤山秋佐夫, 黒川顕, 津田雅孝
Organizer
第8回日本ゲノム微生物学会年会
Place of Presentation
東京
Related Report
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[Presentation] Isolation of phenol-catabolic genes by cultivation-independent functional screening from metagenome of soil artificially polluted by aromatic hydrocarbons2013
Author(s)
Hirofumi Nagayama, Tomonori Sugawara, Ryo Endo, Hiromi Kato, Yoshiyuki Ohtsubo, Yuji Nagata, Hiroshi Mori, Ken, Kurokawa, and Masataka Tsuda
Organizer
2nd Thunen Symposium on Soil Metagenomics: mining and learning from metagenomes plus workshop on bioinformatic tools for soil microbiologists
Place of Presentation
Braunschweig, Germany
Related Report
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[Presentation] Design and experimental validation of a novel non-degenerate universal primer set that amplifies prokaryotic 16S rRNA genes with a low possibility to amplify eukaryotic rRNA genes2013
Author(s)
Hiroshi Mori, Fumito Maruyama, Hiromi Kato, Atsushi Toyoda, Ayumi Dozono, Yoshiyuki Ohtsubo, Yuji Nagata, Asao Fujiyama, Masataka Tsuda, and Ken Kurokawa
Organizer
2nd Thunen Symposium on Soil Metagenomics: mining and learning from metagenomes plus workshop on bioinformatic tools for soil microbiologists
Place of Presentation
Braunschweig, Germany
Related Report
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