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Cell biologically understanding high productivity of protein secretion in Koji mold

Research Project

Project/Area Number 25292045
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionThe University of Tokyo

Principal Investigator

Kitamoto Katsuhiko  東京大学, 農学生命科学研究科, 研究員 (20272437)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords麹菌 / セルファクトリー / タンパク質生産 / オートファジー / タンパク質分泌 / 細胞工場 / 分泌 / 変異株 / ストレス顆粒
Outline of Final Research Achievements

Koji mold (Aspergillus oryzae) is an important microorganism used to brew sake etc in Japan. Koji mold is also useful for the protein production such as enzymes. Its safety is guaranteed due to its long history of use in the food manufacturing industry, and much attention is gained as an attractive host for the useful protein production used in various foods and medical treatments, etc. In this research we tried to elucidate high capability of protein secretion with koji mold by molecular and cellular biology techniques and by making good use of genome information, for the purpose of using as a cell factoryfor useful protein production. Finally I could get following results; 1)functional analysis of cargo receptor genes, 2) Elucidation of autophagy related genes, 3) Discovery of filamentous fungi specific autophagy, nucleophagy, and 4) Identification of a new gene responsible for hyper secretion.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (33 results)

All 2016 2015 2014 2013 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Acknowledgement Compliant: 4 results) Presentation (18 results) (of which Invited: 3 results) Book (4 results) Remarks (2 results)

  • [Journal Article] Modulating endoplasmic reticulum-Golgi cargo receptors for improving secretion of carrier-fused heterologous proteins in the filamentous fungus Aspergillus oryzae.2015

    • Author(s)
      Hoang HD, Maruyama J, Kitamoto K.
    • Journal Title

      Appl Environ Microbiol.

      Volume: 81 Issue: 2 Pages: 533-543

    • DOI

      10.1128/aem.02133-14

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of a genome editing technique using the CRISPR/Cas9 system in the industrial filamentous fungus Aspergillus oryzae2015

    • Author(s)
      T. Katayama, Y. Tanaka, T. Okabe, H. Nakamura, W. Fujii, K. Kitamoto, J. Maruyama
    • Journal Title

      Biotechnol. Lett.

      Volume: 38 Issue: 4 Pages: 637-642

    • DOI

      10.1007/s10529-015-2015-x

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of AoAtg11 in selective autophagy in the filamentous fungus Aspergillus oryzae2015

    • Author(s)
      T. Tadokoro, T. Kikuma, K. Kitamoto
    • Journal Title

      Fungal Biol.

      Volume: 119 Issue: 7 Pages: 560-567

    • DOI

      10.1016/j.funbio.2015.03.001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cell biology of the Koji mold Aspergillus oryzae.2015

    • Author(s)
      Kitamoto K.
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 79 Issue: 6 Pages: 863-869

    • DOI

      10.1080/09168451.2015.1023249

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Vesicle trafficking, organelle functions, and unconventional secretion in fungal physiology and pathogenicity.2014

    • Author(s)
      Shoji JY, Kikuma T, Kitamoto K.
    • Journal Title

      Curr Opin Microbiol.

      Volume: 20 Pages: 1-9

    • DOI

      10.1016/j.mib.2014.03.002

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 糸状菌のオートファジーと物質生産2014

    • Author(s)
      菊間隆志、北本勝ひこ
    • Journal Title

      化学と生物

      Volume: 52 Pages: 757-763

    • NAID

      130005105747

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced production of bovine chymosin by autophagy deficiency in the filamentous fungus Aspergillus oryzae2013

    • Author(s)
      J. Yoon, T. Kikuma, J. Maruyama, K. Kitamoto
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 4 Pages: e62512-e62512

    • DOI

      10.1371/journal.pone.0062512

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of Abp1p-interacting proteins involved in endocytosis of the MCC component in A spergillus oryzae2013

    • Author(s)
      Matsuo, K., Higuchi, Y ., Kikuma, T ., Arioka, M., and Kitamoto, K
    • Journal Title

      Fungal Genet. Biol

      Volume: 56 Pages: 125-134

    • DOI

      10.1016/j.fgb.2013.03.007

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Further enhanced production of heterologous proteins by double-gene disruption (ΔAosedD ΔAovps10) in a hyper-producing mutant of Aspergillus oryzae2013

    • Author(s)
      L. Zhu, J. Maruyama, K. Kitamoto
    • Journal Title

      Appl. Microbiol. Biotechnol.

      Volume: 97 Issue: 14 Pages: 6347-6357

    • DOI

      10.1007/s00253-013-4795-z

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 麹菌A. oryzaeにおける明暗および温度の周期的変化に対する応答機構の解析2016

    • Author(s)
      山本 実侑、川田 純毅、溝上 豊、松尾 花枝、丸山 潤一、北本 勝ひこ
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 麹菌実用株におけるゲノム編集と非相同組換え修復欠損を用いた高効率遺伝子操作技術の開発2016

    • Author(s)
      中村 英淳1、片山 琢也1、岡部 知弥、岩下 和裕、藤井 渉、有岡 学、北本 勝ひこ、丸山 潤一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 麹菌A. oryzaeにおける核の自食機構ヌクレオファジーの解析2016

    • Author(s)
      三谷 隆宏、菊間 隆志、北本 勝ひこ
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 麹菌A. oryzaeにおけるオートファジー関連タンパク質AoAtg26の局在解析2016

    • Author(s)
      菊間 隆志、有岡 学、北本 勝ひこ
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 麹菌におけるCRISPR/Cas9 システムによるゲノム編集技術の確立2015

    • Author(s)
      片山 琢也、中村 英淳、田中 勇気、岡部 知弥、藤井 渉、北本 勝ひこ、丸山 潤一
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 『国菌』麹菌の未来 ― ユネスコ無形文化遺産“和食”とともに2015

    • Author(s)
      北本 勝ひこ
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      岡山
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 麹菌A. oryzaeにおけるオートファジー関連タンパク質AoAtg9の推定リン酸化部位変異体の解析2015

    • Author(s)
      藤木 耕平、菊間 隆志、北本 勝ひこ
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      岡山
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 麹菌A. oryzaeにおけるAcyl-CoA binding protein の機能及び特殊な分泌経路の解析2015

    • Author(s)
      川口 航平、菊間 隆志、北本 勝ひこ
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      岡山
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 比較ゲノム解析による麹菌の異種タンパク質高生産に関与する変異遺伝子の同定2014

    • Author(s)
      金 鋒杰、丸山 潤一、北本 勝ひこ
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 麹菌オートファジーのユニークな生理機能とその応用2014

    • Author(s)
      菊間 隆志、北本 勝ひこ
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 麹菌A. oryzaeにおけるオートファジー関連ステロール-グルコシルトランスフェラーゼ様遺伝子Aoatg26の解析2014

    • Author(s)
      菊間 隆志、田所 隆之、北本 勝ひこ
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 麹菌A. oryzaeにおけるオートファジー関連膜タンパク質AoAtg9の機能解析2014

    • Author(s)
      藤木 耕平、菊間 隆志、北本 勝ひこ
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 麹菌A. oryzaeにおける2つのacyl-CoA binding proteinの局在及び機能解析2014

    • Author(s)
      川口 航平、菊間 隆志、北本 勝ひこ
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Adjusting the cargo receptor composition of ER-Golgi secretoty pathway for enhancing heterologous protein production in Aspergillus oryzae2014

    • Author(s)
      Huy Dung Hoang, Jun-ichi Maruyama, Katsuhiko Kitamoto
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 麹菌のPHO36ホモログ破壊株を用いた植物由来オスモチンの生産2014

    • Author(s)
      伊藤 大修、丸山 潤一、永田 宏次、田之倉 優、北本 勝ひこ
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Effect of deleting two putative cargo receptors on heterologous protein production in Aspergillus oryzae2013

    • Author(s)
      Dung Huy Hoang, Jun-ichi Maruyama, Katsuhiko Kitamoto
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] Developing Aspergillus oryzae as a host for heterologous protein production2013

    • Author(s)
      Katsuhiko Kitamoto
    • Organizer
      XI International Fungal Biology Conference
    • Place of Presentation
      Karlsruhe, Germany
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Functional analysis of selective autophagy-related genes, Aoatg11 and Aoatg26 in the filamentous fungus Aspergillus oryzae2013

    • Author(s)
      Takashi Kikuma, Takayuki Tadokoro, Shin Yanagisawa, Katsuhiko
    • Organizer
      XI International Fungal Biology Conference
    • Place of Presentation
      Karlsruhe, Germany
    • Related Report
      2013 Annual Research Report
  • [Book] Stress Biology of Yeasts and Fungi: Application for Industrial Brewing and Fermentation,2015

    • Author(s)
      J. Maruyama, K. Kitamoto
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report
  • [Book] 麹菌 における接合型遺伝子の存在と有性生殖の可能性2015

    • Author(s)
      丸山潤一 、北本勝ひこ
    • Publisher
      シーエムシー出版
    • Related Report
      2015 Annual Research Report
  • [Book] 麹菌におけるオートファジーの生理的役割2015

    • Author(s)
      菊間隆志、北本勝ひこ
    • Publisher
      シーエムシー出版
    • Related Report
      2015 Annual Research Report
  • [Book] 発酵・醸造食品の最前線(麹菌におけるオートファジーの生理的役割)2015

    • Author(s)
      菊間隆志、北本勝ひこ
    • Total Pages
      8
    • Publisher
      シーエムシー出版
    • Related Report
      2014 Annual Research Report
  • [Remarks] 東京大学微生物学研究室

    • URL

      http://park.itc.u-tokyo.ac.jp/Lab_Microbiology/hyousi.html

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 東京大学微生物学研究室

    • URL

      http://park.itc.u-tokyo.ac.jp/Lab_Microbiology/

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

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