Cell biologically understanding high productivity of protein secretion in Koji mold
| Project/Area Number |
25292045
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|
|---|
| Keywords | 麹菌 / セルファクトリー / タンパク質生産 / オートファジー / タンパク質分泌 / 細胞工場 / 分泌 / 変異株 / ストレス顆粒 |
|---|
| Outline of Final Research Achievements |
Koji mold (Aspergillus oryzae) is an important microorganism used to brew sake etc in Japan. Koji mold is also useful for the protein production such as enzymes. Its safety is guaranteed due to its long history of use in the food manufacturing industry, and much attention is gained as an attractive host for the useful protein production used in various foods and medical treatments, etc. In this research we tried to elucidate high capability of protein secretion with koji mold by molecular and cellular biology techniques and by making good use of genome information, for the purpose of using as a cell factoryfor useful protein production. Finally I could get following results; 1)functional analysis of cargo receptor genes, 2) Elucidation of autophagy related genes, 3) Discovery of filamentous fungi specific autophagy, nucleophagy, and 4) Identification of a new gene responsible for hyper secretion.
|
Report
(4 results)
Research Products
(33 results)
