| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Outline of Final Research Achievements |
Streptomyces have the potential to produce more than 30 secondary metabolites; however, the expression of most metabolite biosynthetic gene clusters is cryptic or silent. Indeed, the expression of these genes is conditional and dependent on culture conditions. To activate such gene clusters, many methods have been developed. Combined-culture which is developed by our group is a coculture method to activate secondary metabolism in Streptomyces. The activator strains are mycolic acid-containing bacteria, and about 90% of Streptomyces species show changes in secondary metabolism in combined-culture compared with pure culture. In this project, we revealed that pigment induction was mediated by cell-to-cell physical contact. In addition, we applied combined-culture to new secondary metabolite screening, and then new secondary metabolites (5-aTHQ, chojyalactone, arcyriaflavin E, niizalactam) are discovered. Thus, combined-culture may have applications in natural product-based drug discovery.
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