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Study of Streptomyces conjugation mechanism and the application for artificial genome evolution

Research Project

Project/Area Number 25292047
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionShinshu University

Principal Investigator

KATAOKA Masakazu  信州大学, 学術研究院工学系, 准教授 (90332676)

Co-Investigator(Kenkyū-buntansha) MISHIMA Masaki  首都大学東京, 理工学研究科, 准教授 (70346310)
Co-Investigator(Renkei-kenkyūsha) IKEDA Haruo  北里大学, 感染制御科学府, 教授 (90159632)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywords放線菌 / 接合伝達 / ATPase / ゲノム移行 / TraB / DNAチャネル / 進化 / 接合関連遺伝子群 / 移植 / 線状プラスミド / 環状プラスミド / ttrA / ゲノム
Outline of Final Research Achievements

ATPase activity on the FtsK domain of TraB was essential for the conjugative transfer. All mutations within Walker type domains except T277S abolished the ATPase activity and conjugation. TraB showed specific binding to TraA. Disorder of TraB expression led the decreasing of transfer efficiency. Chromosomal Mobilization Activity (CMA ) between S. lividans strains was extremely enhanced by genomic insertion of the pSN22 tra genes. Deletion of traB or clt (cis-acting locus of transfer) abolished the CMA activity, and deletion of traA decreased the CMA efficiency. Genomic insertion of clt and trans supplying of the tra gene products also enhanced the CMA. Genome insertion of the tra genes also enhanced hetero genomic recombination suggesting that this phenomena would be useful for the next generation genome engineering .

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (27 results)

All 2016 2015 2014 2013 Other

All Presentation (25 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (2 results)

  • [Presentation] 放線菌プラスミドpSN22の接合伝達における必須タンパク質TraBの機能解析2016

    • Author(s)
      矢野嵩紘、宮武徹、片岡正和
    • Organizer
      日本農芸化学会年会
    • Place of Presentation
      札幌 国際会議場
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 線状プラスミドにおける接合伝達機構の解析2016

    • Author(s)
      丸山直也、池田治生、片岡正和
    • Organizer
      日本農芸化学会年会
    • Place of Presentation
      札幌 国際会議場
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 放線菌接合伝達遺伝子によるゲノム移行2016

    • Author(s)
      村端広介、金眞熙、宮武徹、片岡正和
    • Organizer
      日本農芸化学会年会
    • Place of Presentation
      札幌 国際会議場
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Conjugation in modern microbiology2016

    • Author(s)
      Masakazu Kataoka
    • Organizer
      異分野融合ワークショップ: 微生物生命システム研究と合成生物学の融合
    • Place of Presentation
      奈良先端大学院大学
    • Year and Date
      2016-03-16
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 放線菌プラスミド pSN22 の接合伝達における必須タンパク質 TraB の膜局在及び ATPase 活性に関する解析2016

    • Author(s)
      矢野嵩紘、宮武徹、片岡正和
    • Organizer
      日本ゲノム微生物学会年会
    • Place of Presentation
      大岡山 東工大
    • Year and Date
      2016-03-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 放線菌接合伝達遺伝子による染色体移行手法の開発2015

    • Author(s)
      村端広介、金眞熙、宮武徹、片岡正和
    • Organizer
      グラム陽性菌ゲノム機能会議
    • Place of Presentation
      雄琴温泉 雄山荘
    • Year and Date
      2015-08-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Optimization of Gene Transfer by the RK2 between Cross-species2015

    • Author(s)
      Takahiro Yokoi, Mitsuhiro Itaya, Hitrotada Mori, Msakazu Kataoka
    • Organizer
      American Society for Microbiology
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of DNA channel protein, TraB, for Streptomyces conjugation2015

    • Author(s)
      Masakazu Kataoka, Tetsu Miyatake, Mai Kobe
    • Organizer
      American Society for Microbiology
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 放線菌プラスミドpSN22由来接合伝達遺伝子による染色体移行の解析2015

    • Author(s)
      村端広介, Jim Hee Kim, 宮武徹, 横井崇紘, 片岡正和
    • Organizer
      日本農芸化学会 2015年年会
    • Place of Presentation
      岡山県岡山市 岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 放線菌接合伝達性線状プラスミド SAP1 の解析2015

    • Author(s)
      遠藤翔太、池田治生、片岡正和
    • Organizer
      日本ゲノム微生物学会
    • Place of Presentation
      兵庫県 神戸市 神戸大学
    • Year and Date
      2015-03-06 – 2015-03-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] いまどきの遺伝子操作:巨大なDNAの扱いと接合伝達の復権2014

    • Author(s)
      片岡正和
    • Organizer
      極限環境生物学会
    • Place of Presentation
      沖縄県今帰仁村,ホテルベルパライソ
    • Year and Date
      2014-11-03 – 2014-11-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Localization and interaction of TraA and TraB, the essential factor for the conjugative transfer of Streptomyces plasmid pSN22.2014

    • Author(s)
      Masakazu Kataoka, Tetsu Miyatake
    • Organizer
      17th ISBA
    • Place of Presentation
      Audin, Turkey
    • Year and Date
      2014-10-08 – 2014-10-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Old is New? : New horizon of conjugation in the generation of synthetic biology.2014

    • Author(s)
      Masakazu Kataoka
    • Organizer
      Cold Spring Harbor Laboratory meeting, Plasmids: History & Biology,
    • Place of Presentation
      Cold Spring Harbor USA
    • Year and Date
      2014-09-21 – 2014-09-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Old is New?:合成生物学時代における接合伝達2014

    • Author(s)
      片岡正和
    • Organizer
      2014グラム陽性菌ゲノム機能会議
    • Place of Presentation
      鶴岡市 慶應大学
    • Year and Date
      2014-09-03 – 2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Functional analyses of Streptomyces conjugation system: the molecular function and harmonized expression of the DNA channel, TraB.2014

    • Author(s)
      Masakazu Kataoka, Tetsu Miyatake, Mai Kobe
    • Organizer
      IUMS congress 2014
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2014-07-27 – 2014-08-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] ATPase activity and expression of TraB, the DNA channel protein on Streptomyces conjugative plasmid pSN22.2014

    • Author(s)
      神戸麻衣、片岡正和
    • Organizer
      第8回長野ミーティング:生物資源の有効利用を目指して
    • Place of Presentation
      長野・ラフォーレ白馬
    • Related Report
      2013 Annual Research Report
  • [Presentation] Construction of a gene cluster transfer system with linear plasmid SAP1.2014

    • Author(s)
      藤森友真、片岡正和
    • Organizer
      第8回長野ミーティング:生物資源の有効利用を目指して
    • Place of Presentation
      長野・ラフォーレ白馬
    • Related Report
      2013 Annual Research Report
  • [Presentation] Gene transfer method using cross-species conjugal transfer.2014

    • Author(s)
      横井崇紘、片岡正和
    • Organizer
      第8回長野ミーティング:生物資源の有効利用を目指して
    • Place of Presentation
      長野・ラフォーレ白馬
    • Related Report
      2013 Annual Research Report
  • [Presentation] 線状プラスミドSAP1を用いた遺伝子クラスター輸送システムの構築2013

    • Author(s)
      藤森友真、池田治生、片岡正和
    • Organizer
      日本放線菌学会2013年度大会
    • Place of Presentation
      広島・メルパルク広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] 接合伝達機構を用いた大腸菌-枯草菌-放線菌間における遺伝子操作法の確立2013

    • Author(s)
      横井崇紘、片岡正和
    • Organizer
      グラム陽性菌ゲノム機能会議
    • Place of Presentation
      つくば・筑波山ホテル
    • Related Report
      2013 Annual Research Report
  • [Presentation] 放線菌線状プラスミドSAP1を用いた遺伝子クラスター輸送システム2013

    • Author(s)
      藤森友真、池田治生、片岡正和
    • Organizer
      グラム陽性菌ゲノム機能会議
    • Place of Presentation
      つくば・筑波山ホテル
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生合成系操作における接合伝達システムの応用可能性2013

    • Author(s)
      片岡正和
    • Organizer
      日本遺伝学会85回大会
    • Place of Presentation
      横浜・慶應大日吉
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 接合伝達機構を用いたE.coli -B. subtilis間遺伝子操作法の確立2013

    • Author(s)
      横井崇紘、板谷光泰、森浩禎、片岡正和
    • Organizer
      日本分子生物学会第36回大会
    • Place of Presentation
      神戸・ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] 放線菌伝達性プラスミドpSN22上の伝達関連タンパク質TraBの解析2013

    • Author(s)
      神戸麻依、宮武徹、片岡正和
    • Organizer
      日本分子生物学会第36回大会
    • Place of Presentation
      神戸・ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] 線状プラスミドSAP1 を用いたStreptomycesへの遺伝子クラスター導入系の開発とその評価2013

    • Author(s)
      藤森友真、池田治生、片岡正和
    • Organizer
      日本分子生物学会第36回大会
    • Place of Presentation
      神戸・ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Remarks] 片岡研HP

    • URL

      http://www.kankyo.shinshu-u.ac.jp/~kataokalab/

    • Related Report
      2014 Annual Research Report
  • [Remarks] SOAR

    • URL

      http://www.shinshu-u.ac.jp/soar/

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

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