| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
We have constructed a mutant collection of Bifidobacterium using the gene knock-out (KO) technique, that we have established previously. Nine response regulator genes of the two-component regulatory system were knocked out by double cross-over homologous recombination. Then, the transcriptome analyses were performed. As the result one gene KO showed over x4 or 1/4 change of the gene expression level in over 70 genes. These gene are thought to concern Oxgen stress, Acid and bile tolerance and osmotic shock resistance. Thus, we concluded that this gene should be a master switch gene of the symbiosis with animal gut in Bifidobacterium.
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