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Study on the mechanism by which yellow substance increases enzymatic cellulose degradation and isolation of novel yellow substance

Research Project

Project/Area Number 25292049
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionMie University

Principal Investigator

SAKKA Kazuo  三重大学, 生物資源学研究科, 教授 (20154031)

Co-Investigator(Kenkyū-buntansha) 粟冠 真紀子  三重大学, 生物資源学研究科, 学術研究員 (00422882)
五十嵐 康弘  富山県立大学, 工学部, 教授 (20285159)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywords黄色色素 / セルロース / 分解 / Ruminiclostridium / セルロソーム / セルラーゼ / ヘミセルラーゼ / エンドグルカナーゼ / セルロース分解 / Clostridium thermocellum / Clostridium josui / セロビオヒドロラーゼ
Outline of Final Research Achievements

Yellow substance was extracted with acetone from Ruminiclostridium thermocellum cells anaerobically grown on microcrystalline cellulose (Funacel) as the carbon source, purified, and expected to be a carotenoid-like compound by structural analysis. Yellow substance was also extracted from Ruminiclostridium josui cells with 1-propanol but not acetone. R. thermocellum yellow substance-adsorbed cellulose was efficiently hydrolyzed by not only R. thermocellum cellulosome but also R. josui cellulosome. On the other hand, R. josui yellow substance-adsorbed cellulose was efficiently hydrolyzed by R. josui cellulosome but not R. thermocellum cellulosome. These results suggest that the mechanisms by which cellulose degradability is enhanced are different and dependent on the origins of yellow substances.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (10 results)

All 2017 2016 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Recombinant cellulolytic or xylanolytic complex comprising the full-length scaffolding protein RjCipA and cellulase RjCel5B or xylanase RjXyn10C of Ruminiclostridium josui2017

    • Author(s)
      T. Orita, M. Sakka, T. Kimura, K. Sakka
    • Journal Title

      Enzyme and Microbial Technology

      Volume: 97 Pages: 63-70

    • DOI

      10.1016/j.enzmictec.2016.10.021

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Ruminiclostridium josuiのRjCel5BまたはRjXyn10Cと全長骨格タンパク質との複合体形成と活性の比較2017

    • Author(s)
      織田拓、粟冠真紀子、木村哲哉、粟冠和郎
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府・京都市)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] セルロース分解性嫌気性細菌Ruminiclostridium josuiの制限酵素RjoIの特性2016

    • Author(s)
      汪亜運、奥川慶、粟冠真紀子、木村哲哉、粟冠和郎
    • Organizer
      第80回日本生化学会中部支部例会
    • Place of Presentation
      三重大学(三重県・津市)
    • Year and Date
      2016-05-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] 微生物によるセルロース分解- セルラーゼからセルロース分解菌叢まで2016

    • Author(s)
      粟冠和郎
    • Organizer
      名城大学理工談話会
    • Place of Presentation
      名城大学( 愛知県・名古屋市)
    • Year and Date
      2016-03-16
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 微生物によるバイオマス分解-酵素分子からセルロース分解菌叢まで2015

    • Author(s)
      粟冠和郎
    • Organizer
      バイオリマテリアル創製センター 第2回研究成果報告会
    • Place of Presentation
      名城大学( 愛知県・名古屋市)
    • Year and Date
      2015-03-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Effect of yellow affinity substance on cellulolyticactivity of Ruminiclostridium thermocellum (formerly Clostridium thermocellum) celluloslosome and related enzymes2014

    • Author(s)
      S. Kimata, M. Hatanaka, M. Sakka, T. Kimura and K. Sakka
    • Organizer
      Mie Bioforum 2014- Lignocellulose Degradation and Biorefinery
    • Place of Presentation
      合歓の郷ホテル&リゾート( 三重県・志摩市)
    • Year and Date
      2014-11-18
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a host-vector system in Ruminiclostridium josui, formerly Clostridium josui2014

    • Author(s)
      K, Okugawa, M. Sakka, T. Kimura and K. Sakka
    • Organizer
      Mie Bioforum 2014- Lignocellulose Degradation and Biorefinery
    • Place of Presentation
      合歓の郷ホテル&リゾート( 三重県・志摩市)
    • Year and Date
      2014-11-18
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Complete genome sequence of Ruminiclostridium josui, formerly Clostridium josui2014

    • Author(s)
      T. Orita, M. Sakka, T. Kimura and K. Sakka
    • Organizer
      Mie Bioforum 2014- Lignocellulose Degradation and Biorefinery
    • Place of Presentation
      合歓の郷ホテル&リゾート( 三重県・志摩市)
    • Year and Date
      2014-11-18
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 嫌気性細菌の生産する黄色色素(YAS)とセルラーゼ活性に関する研究

    • Author(s)
      粟冠真紀子、木全翔太郎、木村哲哉、粟冠和郎
    • Organizer
      日本農芸化学会中部支部第168回例会
    • Place of Presentation
      名古屋市名古屋大学シンポジオン
    • Related Report
      2013 Annual Research Report
  • [Book] Lignocellulose degradation and Biorefinery2015

    • Author(s)
      Makiko Sakka, Shotaro Kimata, Namika Hatanaka, Kei Okugawa, Taku Orita, Tetsuya Kimura, and Kazuo Sakka
    • Total Pages
      351
    • Publisher
      UNIPACS Co., Ltd.
    • Related Report
      2015 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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