| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
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| Outline of Final Research Achievements |
Sucrose-induced repression of translation (SIRT), which is mediated by upstream open reading frames (uORFs), was initially reported in AtbZIP11. Here, two AtbZIP11 orthologous genes, SlbZIP1 and SlbZIP2, were identified in tomato. SlbZIP1 and SlbZIP2 contained several uORFs in the cDNA 5'-leader regions. The second uORFs were conserved and involved in SIRT. Tomato plants were transformed with binary vectors in which only the main open reading frames (ORFs) of SlbZIP1 and SlbZIP2 were placed under the control of the fruit-specific promoter. Growth and morphology of the resulting transgenic tomato plants were comparable to those of wild-type plants. Transgenic fruits were approximately 1.5-fold higher in sugar content than nontransgenic tomato fruits. In addition, the levels of several amino acids were higher in transgenic fruits than in wild-type fruits. This 'sweetening' technology is broadly applicable to other plants that utilize sucrose as a major translocation sugar.
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