Biomodulation with D-amino acids: molecular mechanism and application
| Project/Area Number |
25292059
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HEMMI Hisashi 名古屋大学, 大学院生命農学研究科, 准教授 (60302189)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | D-アミノ酸 / D－セリン / D-アスパラギン酸 / セリンラセマーゼ / D-セリンデヒドラターゼ / D－アスパラギン酸 / D－セリンデヒドラターゼ / D-アミノ酸スパラギン酸 / ビタミンB６ / 細胞性粘菌 / アスパラギン酸ラセマーゼ / ピリドキサルリン酸 |
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| Outline of Final Research Achievements |
In the current studies, we intended to clarify the molecular bases of the physiological functions of D-amino acids, especially D-Ser and D-Asp. We clarified the reaction mechanism of D-Ser metabolizing enzymes、serine racemase and D-serine dehydratase with focusing on the elimination of the hydroxyl group of substrate. We examined the role of D-Ser and serine racemase in the cellular slime mold, Dictyostelium discoideum, and found that serine racemase probably regulates L-serine concentration. We tried to clarify the synthetic enzyme of D-Asp in mammals, and found that Got1l1 is not an aspartate racemase but a L-glutamate transaminase. We tried to improve the sensitivity of our enzymatic D-Ser assay system, and succeeded to increase it ten times. We attempted to regulate the brain D-Ser concentration with PEGylated D-serine dehydratase. We also studied the protein structure recognizing D-amino acids.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Role of the aminotransferase domain in Bacillus subtilis GabR, a pyridoxal 5'-phosphate-dependent transcriptional regulator2015
Author(s)
Okuda K., Kato S., Ito T., Shiraki S., Kawase Y., Goto M., Kawashima S., Hemmi H., Fukada H., Yoshimura T
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Journal Title
Molecular Microbiology
Volume: 95
Issue: 2
Pages: 245-257
DOI
Related Report
Peer Reviewed
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[Presentation] Serine Racemase in Dictyostelium discoideum2014
Author(s)
Saki Kobayashi, Natsuki HAMAUCHI, Tomokazu ITO, Hisashi HEMMI, Yukie SATO, Tamao SAITO, Tohru YOSHIMURA
Organizer
The 2nd International Conference of D-Amino Acid Research
Place of Presentation
宇都宮市
Year and Date
2014-11-05 – 2014-11-07
Related Report
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