| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
In the current studies, we intended to clarify the molecular bases of the physiological functions of D-amino acids, especially D-Ser and D-Asp. We clarified the reaction mechanism of D-Ser metabolizing enzymes、serine racemase and D-serine dehydratase with focusing on the elimination of the hydroxyl group of substrate. We examined the role of D-Ser and serine racemase in the cellular slime mold, Dictyostelium discoideum, and found that serine racemase probably regulates L-serine concentration. We tried to clarify the synthetic enzyme of D-Asp in mammals, and found that Got1l1 is not an aspartate racemase but a L-glutamate transaminase. We tried to improve the sensitivity of our enzymatic D-Ser assay system, and succeeded to increase it ten times. We attempted to regulate the brain D-Ser concentration with PEGylated D-serine dehydratase. We also studied the protein structure recognizing D-amino acids.
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