Advanced use of vitamin D hydroxylases
Project/Area Number |
25292062
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Applied biochemistry
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Research Institution | Toyama Prefectural University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KANO KENJI 京都大学, 農学研究科, 教授 (10152828)
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Co-Investigator(Renkei-kenkyūsha) |
IKUSHIRO SHINICHI 富山県立大学, 工学部, 准教授 (50244679)
YASUTAKE YOSHIAKI 産業技術総合研究所, 生物プロセス研究部門, 研究員 (20415756)
TAMURA TOMOHIRO 産業技術総合研究所, 生物プロセス研究部門, 研究部長 (30357488)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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Keywords | ビタミンD / シトクロムP450 / ビタミンD水酸化酵素 / ビタミンD受容体 / 高感度検出系 / 酵素電極 / バイオコンバージョン |
Outline of Final Research Achievements |
We have successfully produced the active form of vitamin D2 by a triple variant of CYP105A1. Many metabolites of natural or chemically synthesized vitamin D were also produced by bacterial or mammalian vitamin D hydroxylases to use them as authentic standards. In addition, we have developed a novel and highly sensitive system to detect serum 25-hydroxyvitamin D3.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Synthesis and metabolic studies of 1α,2α,25-, 1α,4α,25- and 1α,4β,25- trihydroxyvitamin D3.2015
Author(s)
Takano M, Sawada D, Yasuda K, Nishikawa M, Takeuchi A, Takagi KI, Horie K,Reddy GS, Chen TC, Sakaki T, Kittaka A.
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Journal Title
J Steroid Biochem Mol Biol.
Volume: 148
Pages: 34-37
Related Report
Peer Reviewed
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[Journal Article] Synthesis and Biological Activity of 1α,2α,25-Trihydroxyvitamin D3: Active Metabolite of 2α -(3-Hydroxypropoxy)-1a,25-dihydroxyvitamin D3 by Human CYP3A42014
Author(s)
M. Takano, S. Ohya, K. Yasuda, M. Nishikawa, A. Takeuchi, D. Sawada D, Saito H, Takenouchi K, Sakaki T, Kittaka A
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Journal Title
Chemical and Pharmaceutical Bulletin
Volume: 62
Pages: 182-184
Related Report
Peer Reviewed
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[Journal Article] 1,25-(OH)2D-24-hydroxylase (CYP24A1) deficiency as a cause of nephrolithiasis2013
Author(s)
G. Nesterova, M. Christine, K. Yasuda, T. Sakaki, T. Vilboux, C. Ciccone, R. Horst, Y Huang, G. Golas, W. Introne, M. Huizing, D. Adams, C.F. Boerkoel, M.T.Collins, W.A. Gahl
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Journal Title
Clinical Journal of the American Society of Nephrology
Volume: 8
Issue: 4
Pages: 649-657
DOI
Related Report
Peer Reviewed
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[Presentation] Protein engineering of CYP105A1 to produce active forms of2015
Author(s)
Yuya Yogo, Kaori Yasuda, Teisuke Takita, Hiroshi Sugimoto, Yoshitsugu Shiro, Hiroki Mano, Keiko Hayashi, Masaki Kamakura, Miho Ohta, Shinichi lkushiro, Kiyoshi Yasukawa, Toshiyuki Sakaki
Organizer
The 19th International Conference on Cytochrome P450
Place of Presentation
Tokyo
Year and Date
2015-06-12
Related Report
Int'l Joint Research
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[Presentation] Microbial production of hydroxylated metabolites of vitamin D2014
Author(s)
Kaori Yasuda, Yuya Yogo, Keiko Hayashi, Mariko Endo, Masaki Kamakura, Miho Ohta, Yoshiaki Yasutake, Tomohiro Tamura, Shinichi Ikushiro and Toshiyuki Sakaki
Organizer
12th International Symposium on cytochrome P450 Biodiversity and Biotechnology
Place of Presentation
京都
Year and Date
2014-09-24 – 2014-09-28
Related Report
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[Presentation] Protein engineering of actinomycete CYP105A12014
Author(s)
Teisuke Takita, Kaori Yasuda, Yuya Yogo, Hiroshi Sugimoto, Yoshitsugu Shiro Keiko Hayashi, Hiroki Mano, Masaki Kamakura, Miho Ohta, Shinichi Ilkushiro, Kiyoshi Yasukawa, Toshiyuki Sakaki
Organizer
12th International Symposium on cytochrome P450 Biodiversity and Biotechnology
Place of Presentation
京都
Year and Date
2014-09-24 – 2014-09-28
Related Report
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