| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Outline of Final Research Achievements |
Plausible biosynthetic precursors of strigolactones (SLs) were applied to SL-producing plants and conversion of the precursors to SLs was analyzed. The conversion of carlactone (CL) to carlactonoic acid (CLA) was a common reaction among the plants. Sorghum converted CL and CLA to 5-deoxystrigol (5-DS) and sorgomol, and 5-DS to sorgomol. Moonseed converted CL and CLA to strigol but not to 5-DS. The plant did not convert 5-DS to strigol, suggesting that 5-DS is not a precursor of strigol in moonseed. Coincidently, moonseed converted exogenously applied CL to an unknown metabolite. Molecular mass of the metabolite was larger than that of CL by 16 and its major fragment ion smaller than that of CL by 2, suggesting that it is most probably a hydroxylated product of CL. Similarly, 4-deoxyorobanchol is not a precursor of orobanchol in cowpea. These results demonstrate that biosynthetic pathway of hydroxyl SLs does not necessarily pass through their respective deoxy SL as a precursor.
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