Elucidation of biosynthetic pathway from carlactone to strigolactones
Project/Area Number |
25292065
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Bioorganic chemistry
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Research Institution | Kobe University |
Principal Investigator |
SUGIMOTO Yukihiro 神戸大学, (連合)農学研究科(研究院), 教授 (10243411)
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Co-Investigator(Kenkyū-buntansha) |
中嶌 瞳 神戸大学, (連合)農学研究科(研究院), 研究員 (30598777)
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Co-Investigator(Renkei-kenkyūsha) |
MIZUTANI Masaharu 神戸大学, 大学院農学研究科, 准教授 (60303898)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
|
Keywords | カルラクトン / ストライゴラクトン / 生合成 / ソルゴモール / 5-デオキシストライゴール / 4-デオキシオロバンコール / 5-デオキシストライゴール |
Outline of Final Research Achievements |
Plausible biosynthetic precursors of strigolactones (SLs) were applied to SL-producing plants and conversion of the precursors to SLs was analyzed. The conversion of carlactone (CL) to carlactonoic acid (CLA) was a common reaction among the plants. Sorghum converted CL and CLA to 5-deoxystrigol (5-DS) and sorgomol, and 5-DS to sorgomol. Moonseed converted CL and CLA to strigol but not to 5-DS. The plant did not convert 5-DS to strigol, suggesting that 5-DS is not a precursor of strigol in moonseed. Coincidently, moonseed converted exogenously applied CL to an unknown metabolite. Molecular mass of the metabolite was larger than that of CL by 16 and its major fragment ion smaller than that of CL by 2, suggesting that it is most probably a hydroxylated product of CL. Similarly, 4-deoxyorobanchol is not a precursor of orobanchol in cowpea. These results demonstrate that biosynthetic pathway of hydroxyl SLs does not necessarily pass through their respective deoxy SL as a precursor.
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Report
(5 results)
Research Products
(13 results)
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[Journal Article] Ueno, K., Ishiwa, S., Nakashima, H., Mizutani, M., Takikawa, H., Sugimoto, Y.: Regioselective and stereospecific hydroxylation of GR24 by Sorghum bicolor and evaluation of germination inducing activities of hydroxylated GR24 stereoisomers toward seeds of Striga species2015
Author(s)
Ueno, K., Ishiwa, S., Nakashima, H., Mizutani, M., Takikawa, H., Sugimoto, Y.
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Journal Title
Bioorganic and Medicinal Chemistry
Volume: 23
Issue: 18
Pages: 6100-6110
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Heliolactone, a non-sesquiterpene lactone germination stimulant for root parasitic weeds from sunflower2014
Author(s)
Ueno, K., Furumoto, T., Umeda, S., Mizutani, M., Takikawa, H., Sugimoto, Y.
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Journal Title
Phytochemistry
Volume: 108
Pages: 122-128
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] The bioconversion of 5-deoxystrigol to sorgomol by the sorghum, Sorghum bicolor (L.) Moench.2013
Author(s)
Motonami, N., Ueno, K., Nakashima, H., Nomura, S., Mizutani, M., Takikawa, H., Sugimoto, Y.
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Journal Title
Phytochemistry
Volume: 93
Pages: 41-48
DOI
Related Report
Peer Reviewed
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[Presentation] Heliolactone, a non-sesquiterpene lactone germination stimulant for root parasitic weeds from sunflower2015
Author(s)
Sugimoto, Y., Ueno, K., Furumoto, T., Umeda, S., Mizutani, M., Takikawa, H., Batchvarova, R.
Organizer
The 1st International Congress on Strigolactones
Place of Presentation
Wageningen, The Netherlands
Year and Date
2015-03-01 – 2015-03-05
Related Report
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[Presentation] Oxidative metabolism of 5-deoxystrigol in plants2015
Author(s)
Ueno, K., Nakashima, H., Mizutani, M., Takikawa, H., Sugimoto, Y.
Organizer
The 1st International Congress on Strigolactones
Place of Presentation
Wageningen, The Netherlands
Year and Date
2015-03-01 – 2015-03-05
Related Report
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[Presentation] Regioselective and stereospecific hydroxylation of GR24bby sorghum2015
Author(s)
Ishiwa, S., Ueno, K., Nakashima, H., Mizutani, M., Takikawa, H., Sugimoto, Y.
Organizer
The 1st International Congress on Strigolactones
Place of Presentation
Wageningen, The Netherlands
Year and Date
2015-03-01 – 2015-03-05
Related Report
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