| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
We studied molecular mechanism of plant immune responses induced by effector proteins derived from pathogenic bacteria. To identify the effector derived from pathogenic bacteria, the transposon-tagging mutants of the pathogenic bacteria were generated. The screening based on immune induction revealed that IPPT-disruption mutant lost the ability of immune induction in cultured rice cells. The transient expression of IPPT derived from rice-avirulent strain in the rice protoplasts caused immune reactions, but IPPT derived from rice-virulent strain did not. The sequence analysis of IPPT genes revealed that substitution of 13 amino acids exists between the virulent and the avirulent strains, suggesting that there is the specific perception system that recognizes the avirulent IPPT. Moreover, our experiment showed that Ca2+-dependent protein kinases are involved in a signal transduction of the IPPT perception.
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