Molecular mechanism of plant immune responses induced by effector proteins derived from pathogenic bacteria
| Project/Area Number |
25292067
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Nagahama Institute of Bio-Science and Technology |
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Principal Investigator |
SAI KOUSHOKU 長浜バイオ大学, バイオサイエンス研究科, 教授 (00263442)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO AKITSUGU 長浜バイオ大学, バイオサイエンス研究科, 教授 (30174775)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | 植物免疫 / エフェクター / 過敏感細胞死 / 特異的認識 / 宿主特異性決定 / 活性酸素 / タンパク質リン酸化 / 分子間相互作用 / タンパク質相互作用 / 病徴形成 / Hrp分泌機構 / ETI / プロテインキナーゼ / 細胞内情報伝達 / ゲノム配列 / PAMP / PTI |
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| Outline of Final Research Achievements |
We studied molecular mechanism of plant immune responses induced by effector proteins derived from pathogenic bacteria. To identify the effector derived from pathogenic bacteria, the transposon-tagging mutants of the pathogenic bacteria were generated. The screening based on immune induction revealed that IPPT-disruption mutant lost the ability of immune induction in cultured rice cells. The transient expression of IPPT derived from rice-avirulent strain in the rice protoplasts caused immune reactions, but IPPT derived from rice-virulent strain did not. The sequence analysis of IPPT genes revealed that substitution of 13 amino acids exists between the virulent and the avirulent strains, suggesting that there is the specific perception system that recognizes the avirulent IPPT. Moreover, our experiment showed that Ca2+-dependent protein kinases are involved in a signal transduction of the IPPT perception.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] CD2-1, the C-terminal region of flagellin, modulates the induction of immune responses in rice.2015
Author(s)
Katsuragi, Y., Takai, R., Furukawa, T., Hirai, H., Morimoto, T., Katayama, T., Murakami, T. and Che, F. S.
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Journal Title
Mol. Plant Microb. Interact
Volume: 28
Pages: 648-658
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Glycan moiety of flagellin in Acidovorax avenae K1 prevents the recognition by rice that causes the induction of immune responses.2014
Author(s)
Hirai, H., Takai, R., M., Kondo, M., Furukawa, T., Hishiki, T., Takayama, S., Che, F. S.
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Journal Title
Plant Signaling & Behavior
Volume: 9
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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