Functional analysis of ER-60, an ER chaperone by gene mamipulation and crystal structure analysis
| Project/Area Number |
25292070
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Kyoto University |
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Principal Investigator |
Reiko Urade 京都大学, (連合)農学研究科(研究院), 教授 (90167289)
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| Co-Investigator(Kenkyū-buntansha) |
裏出 良博 筑波大学, 学内共同利用施設等, 教授 (10201360)
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| Co-Investigator(Renkei-kenkyūsha) |
Lazarus Michael 筑波大学, 国際統合睡眠医科学研究機構, 若手フェロー (80469650)
Cherasse Yoan 筑波大学, 国際統合睡眠医科学研究機構, 研究員 (60544319)
Nagata Nanae 筑波大学, 国際統合睡眠医科学研究機構, 研究員 (80390805)
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| Research Collaborator |
Higashino Yuki 京都大学, 大学院農学研究科, 技術職員
Natsusaki Motonori 京都大学, 大学院農学研究科, 研究員
Okuda Aya 京都大学, 大学院農学研究科・学術振興会, 特別研究員PD
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | アミロイドβペプチド / 小胞体 / 分子シャペロン / 結晶構造解析 / アルツハイマー病 / タンパク質結晶構造解析 / 酵素 / ノックアウトマウス / ER-60 |
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| Outline of Final Research Achievements |
The project was performed to clarify the relationship between Alzheimer’s dementia and a molecular chaperon located in the endoplasmic reticulum, ER-60. We demonstrated that ER-60 has protective effects against toxicity of amyloid beta peptides by using brain neuron-specific ER-60 knockout mice. The function is exerted through inhibition of polymerization of amyloid beta peptides by b-b' domain of ER-60. The amino acid residues essential for
binding to amyloid beta peptides were identified by X-ray structural analysis of a complex of the b-b’ fragment of ER-60 and amyloid beta peptide. In addition, it was suggested that amyloid beta peptides bind to b-b' domain in a flexible manners.
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Report
(4 results)
Research Products
(5 results)
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[Journal Article] Cooperative Protein Folding by Two Protein Thiol Disulfide Oxidoreductases and ERO1 in Soybean2016
Author(s)
Matsusaki M, Okuda A, Masuda T, Koishihara K, Mita R, Iwasaki K, Hara K, Naruo Y, Hirose A, Tsuchi Y, Urade R
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Journal Title
Plant Physiology
Volume: 170
Issue: 2
Pages: 774-774
DOI
Related Report
Peer Reviewed / Open Access
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