Activation of endogenous defense mechanisms by catabolites of dietary quercetin glycosides

• Project/Area Number: 25292073
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Okayama University
• Principal Investigator: NAKAMURA YOSHIMASA 岡山大学, その他の研究科, 教授 (60324381)
• Co-Investigator(Kenkyū-buntansha): KATO YOJI 兵庫県立大学, 環境人間学部, 教授 (30305693)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
• Keywords: ポリフェノール / ケルセチン / 生体内代謝物 / 分子標的 / DOPAC / クリックケミストリー / ビオチンラベル化

Outline of Final Research Achievements

The purpose of this study was to clarify the physiological significance of the major phenolic acid catabolites of quercetin 4´-glucoside (Q4´G) from onion through studies on their activation of endogenous defense mechanisms such as the intracellular antioxidant activity and phase II cytoprotective enzymes. DOPAC significantly inhibited the hydrogen peroxide-induced cytotoxicity in hepatocytes with the enhanced expression of the phase II enzymes, which was potentiated by the combination with another catabolite of Q4´G. Various DOPAC-labelled intracellular proteins were detected using a novel tag-free DOPAC probe with the click chemistry. A pulldown assay identified Keap1 and aryl hydrocarbon receptor (AhR) as the target proteins for the phase 2 enzyme up-regulation by DOPAC. Additional researches suggested that optimal polyphenol supplementation could confer beneficial effects but high doses elicit adverse effects in an electrophilic reaction-dependent manner.

Research Products

• [Journal Article] A mushroom-derived amino acid, ergothioneine, is a potential inhibitor of inflammation-related DNA halogenation. (2016)
  • Author(s): Asahi, T., Wu, X., Shimoda, H., Hisaka, S., Harada, E., Kanno, T., Nakamura, Y., Kato, Y., Osawa, T.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 80 Issue: 2 Pages: 313-317
  • DOI: 10.1080/09168451.2015.1083396
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effects of Sodium Chloride and Ascorbic Acid on the Formation of Isothiocyanates in Two Types of Pickled Cruciferous Vegetables (Turnip, Chinese cabbage) (2016)
  • Author(s): 宮澤紀子・阿部雅子・木村典代・松岡寛樹・田中 進・森光康次郎・中村宜督・綾部園子・小澤好夫
  • Journal Title: Journal of Cookery Science of Japan Volume: 49 Issue: 2 Pages: 138-146
  • DOI: 10.11402/cookeryscience.49.138
  • NAID: 130005150149
  • ISSN: 1341-1535, 2186-5787
  • Peer Reviewed
• [Journal Article] Plant polyphenols as a double-edged sword in health promotion: Lessons from the experimental models using simple phenolic acids. (2016)
  • Author(s): Nakamura, Y.
  • Journal Title: Agri-Biosci. Monogr. Volume: 6 Issue: 1 Pages: 1-57
  • DOI: 10.5047/agbm.2016.00601.0001
  • NAID: 40021125094
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Allyl isothiocyanate induces stomatal closure in Vicia faba. (2015)
  • Author(s): Sobahan, M.A., Akter, N., Okuma, E., Uraji, M., Ye, W., Mori, I.C., Nakamura, Y., Murata, Y.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 79 Issue: 10 Pages: 1737-1742
  • DOI: 10.1080/09168451.2015.1045827
  • Peer Reviewed
• [Journal Article] Specific role of taurine in the 8-brominated-2´-deoxyguanosine formation. (2015)
  • Author(s): Asahi, T., Nakamura, Y., Kato, Y., Osawa, T.
  • Journal Title: Arch. Biochem. Biophys. Volume: 586 Pages: 45-50
  • DOI: 10.1016/j.abb.2015.10.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Benzyl isothiocyanate inhibits IL-13 expression in human basophilic KU812 cells. (2015)
  • Author(s): Yue Tang, Naomi Abe, Motoko Yoshimoto, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 79 Issue: 1 Pages: 159-163
  • DOI: 10.1080/09168451.2014.963503
  • Peer Reviewed
• [Journal Article] Ascorbic acid synergistically potentiates phloxine-B induced photc cytotoxicity in human acute promyelocytic leukemia cells. (2014)
  • Author(s): Hang Qi, Qian Wu, Naomi A be, Shunya Saiki, Beiwei Zhu, Yoshiyuki Murata, Yoshim asa Nakamura
  • Journal Title: Journal of Biochemical and Molecular Toxicol ogy Volume: (印刷中)(20110104受理) Issue: 4 Pages: 167-173
  • DOI: 10.1002/jbt.21549
  • Peer Reviewed
• [Journal Article] Thiol modification by bioactivated polyphenols and its potential role in skin inflammation. (2014)
  • Author(s): Nakamura Y, Ishii T, Abe N, Murata Y.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 78 Issue: 6 Pages: 1067-1070
  • DOI: 10.1080/09168451.2014.905190
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] (-)-Epigallocatechin-3-gallate ameliorates photodynamic therapy responses in an in vitro T lymphocyte model. (2014)
  • Author(s): Qi H, Abe N, Zhu B, Murata Y, Nakamura Y.
  • Journal Title: Phytother. Res. Volume: 28 Issue: 10 Pages: 1486-1491
  • DOI: 10.1002/ptr.5152
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nuclear factor-kappaB sensitizes to benzyl isothiocyanate-induced antiproliferation in p53-deficient colorectal cancer cells. (2014)
  • Author(s): Naomi Abe, De-xing Hou, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura
  • Journal Title: Cell Death and Disease Volume: 5 Issue: 11 Pages: e1534-e1534
  • DOI: 10.1038/cddis.2014.495
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Tea Catechins Inhibit Cell Proliferation Through Hydrogen Peroxide-Dependent and -Independent Pathways in Human T lymphocytic Leukemia Jurkat Cells (2014)
  • Author(s): Tang Y, Abe N, Qi H, Zhu B, Murata Y, Nakamura Y.
  • Journal Title: Food Science and Technology Research Volume: 20 Issue: 6 Pages: 1245-1249
  • DOI: 10.3136/fstr.20.1245
  • NAID: 130004848141
  • ISSN: 1344-6606, 1881-3984
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Thiol modification by bioactivated polyphenols and its potential role in skin inflammation. (2014)
  • Author(s): Nakamura, Y., Ishii T., Abe, N., Murata, Y.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 78
  • Peer Reviewed
• [Journal Article] Lower Photostability of Capsanthin Dispersed in an Aqueous Solution (2013)
  • Author(s): Nishino, M.*, Sakata, M., Murata, Y., Nakamura, Y.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 6 Pages: 1313-1316
  • DOI: 10.1271/bbb.130033
  • NAID: 10031184702
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Effects of Emulsifiers on the Photostability of Lycopene (2013)
  • Author(s): Nishino, M., Sakata, M., Murata, Y., Nakamura, Y.
  • Journal Title: Food Science and Technology Research Volume: 19 Issue: 6 Pages: 983-987
  • DOI: 10.3136/fstr.19.983
  • NAID: 130003392388
  • ISSN: 1344-6606, 1881-3984
  • Peer Reviewed
• [Presentation] ケルセチン配糖体代謝物の新規タグフリープローブの開発 (2016)
  • Author(s): ○中島 清花、劉 哲、山口 佑也、齋木 俊也、中村 俊之、宗正 晋太郎、村田 芳行、中村 宜督
  • Organizer: 日本農芸化学会2015年大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-03-28
• [Presentation] The regulating role of nuclear factor-kappaB in benzyl isothiocyanate-induced antiproliferation in p53-deficient colorectal cancer cells (2015)
  • Author(s): N. Abe, D.-X. Hou, S. Munemasa, Y. Murata, ◯Y. Nakamura
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2015
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Nuclear factor-kappaB as a potential target for antiproliferation induced by benzyl isothiocyanate in p53-deficient colorectal cancer cells (2015)
  • Author(s): N. Abe, D.-X. Hou, S. Munemasa, Y. Murata, ◯Y. Nakamura
  • Organizer: 6th International Conference on Food Factors
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2015-11-22
  • Int'l Joint Research / Invited
• [Presentation] A novel tag-free probe for targeting molecules interacted with flavonoid catabolites (2015)
  • Author(s): S. Nakashima, Z. Liu, Y. Yamaguchi, S. Saiki, Y. Murata, ◯Y. Nakamura
  • Organizer: 7th International Conference on Polyphenols and Health
  • Place of Presentation: Tours, France
  • Year and Date: 2015-10-27
  • Int'l Joint Research
• [Presentation] フラボノイド配糖体代謝物による生体防御機構の活性化 (2014)
  • Author(s): 中村宜督
  • Organizer: 第8回Antioxidant Unit（AOU）研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-11-26
  • Invited
• [Presentation] 食品成分ベンジルイソチオシアネートは動原体構成タンパクMis12の分解を介してがん細胞の増殖を阻害する (2014)
  • Author(s): Naomi Abe, Narumi Kunisue, Shintaro Munemasa, Yoshiyuki Murata, Hisao Moriya and Yoshimasa Nakamura
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 食品に含まれる抗酸化物質の機能性と安全性-基礎研究の視点から- (2014)
  • Author(s): 中村宜督
  • Organizer: 健康食品管理士会中国支部研修会 市民公開講座
  • Place of Presentation: 岡山
  • Year and Date: 2014-07-12
  • Invited
• [Presentation] Benzyl isothiocyanate inhibits IL-13 expression in human basophil KU812 cells (2014)
  • Author(s): Yue Tang, Sho Naito, Naomi Abe, Yoshiyuki Murata, Yoshimasa Nakamura
  • Organizer: 日本農芸化学会中四国支部第39回講演会
  • Place of Presentation: 福山
  • Year and Date: 2014-05-31
• [Presentation] アブラナ科野菜の健康機能とその分子機構
  • Author(s): 中村宜督
  • Organizer: 第46回日本栄養・食糧学会中国・四国支部大会 特別講演：『食品成分による健康保持』
  • Place of Presentation: 山口
  • Invited
• [Remarks] http://yossan24okayama.jimdo.com/
• [Remarks] http://yossan24okayama.jimdo.com/