Activation of endogenous defense mechanisms by catabolites of dietary quercetin glycosides
| Project/Area Number |
25292073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATO YOJI 兵庫県立大学, 環境人間学部, 教授 (30305693)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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| Keywords | ポリフェノール / ケルセチン / 生体内代謝物 / 分子標的 / DOPAC / クリックケミストリー / ビオチンラベル化 |
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| Outline of Final Research Achievements |
The purpose of this study was to clarify the physiological significance of the major phenolic acid catabolites of quercetin 4´-glucoside (Q4´G) from onion through studies on their activation of endogenous defense mechanisms such as the intracellular antioxidant activity and phase II cytoprotective enzymes. DOPAC significantly inhibited the hydrogen peroxide-induced cytotoxicity in hepatocytes with the enhanced expression of the phase II enzymes, which was potentiated by the combination with another catabolite of Q4´G. Various DOPAC-labelled intracellular proteins were detected using a novel tag-free DOPAC probe with the click chemistry. A pulldown assay identified Keap1 and aryl hydrocarbon receptor (AhR) as the target proteins for the phase 2 enzyme up-regulation by DOPAC. Additional researches suggested that optimal polyphenol supplementation could confer beneficial effects but high doses elicit adverse effects in an electrophilic reaction-dependent manner.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] A mushroom-derived amino acid, ergothioneine, is a potential inhibitor of inflammation-related DNA halogenation.2016
Author(s)
Asahi, T., Wu, X., Shimoda, H., Hisaka, S., Harada, E., Kanno, T., Nakamura, Y., Kato, Y., Osawa, T.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 80
Issue: 2
Pages: 313-317
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Allyl isothiocyanate induces stomatal closure in Vicia faba.2015
Author(s)
Sobahan, M.A., Akter, N., Okuma, E., Uraji, M., Ye, W., Mori, I.C., Nakamura, Y., Murata, Y.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 79
Issue: 10
Pages: 1737-1742
DOI
Related Report
Peer Reviewed
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[Journal Article] Lower Photostability of Capsanthin Dispersed in an Aqueous Solution2013
Author(s)
Nishino, M.*, Sakata, M., Murata, Y., Nakamura, Y.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 77
Issue: 6
Pages: 1313-1316
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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