| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Outline of Final Research Achievements |
Cryo-preservation methods of embryogenic cells were developed for being ready to produce transgenic Cryptomeria japonica. Transgenic C. japonica expressing barnase controlled by male cone specific promoter were stably male sterility and grew identically to non-transgenic trees in a greenhouse. By comprehensive analysis of expressing genes at female reproductive organs, we collected the genes which did not expressed at vegetative organs, and elucidated spatiotemporal expression pattern of the genes associated with female reproductive development. These results will be useful for the production of non-pollen C. japonica without transgene dispersal by seeds.
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