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New approaches towards high-value added lignin production by the combination of metabolic engineering and organic chemistry

Research Project

Project/Area Number 25292104
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Wood science
Research InstitutionKyoto University

Principal Investigator

Toshiaki Umezawa  京都大学, 生存圏研究所, 教授 (80151926)

Co-Investigator(Kenkyū-buntansha) SUZUKI Shiro  京都大学, 生存圏研究所, 助教 (70437268)
TOBIMATSU Yuki  京都大学, 生存圏研究所, 准教授 (20734221)
Co-Investigator(Renkei-kenkyūsha) SHIBATA Daisuke  かずさDNA研究所, 部長 (10370925)
SUZUKI Hideyuki  かずさDNA研究所, 主任研究員 (80276162)
KAWAMOTO Haruo  京都大学, 大学院エネルギー科学研究科, 准教授 (80224864)
KIKUCHI Jun  理化学研究所, 先端NMRメタボロミクスチーム, チームリーダー (00321753)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Keywordsリグニン / 代謝工学 / 有機化学 / イネ科植物 / 還元分解 / 芳香核構造単純化 / 有機工業原材料 / 芳香族資源
Outline of Final Research Achievements

Towards development of technologies for sustainable utilization of renewable lignocellulose biomass, development of high-value added products from lignin is critically important. To do so, simplification of lignin structures are of fundamental importance. Herein, we developed a new reductive reaction to decrease the complexity of lignin aromatic composition. In addition, we engineered lignin biosynthesis in rice to give rise to transgenic rice plants where aromatic composition of lignins was largely simplified yielding lignins composed mainly of p-hydroxyphenyl, guaiacyl or syringyl units. New findings on reactivity of the engineered ligninocelluloses was also obtained. Taken together, this study provided basic knowledges for future lignocellulose utilization.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (34 results)

All 2016 2015 2014 Other

All Presentation (30 results) (of which Int'l Joint Research: 3 results,  Invited: 13 results) Book (1 results) Remarks (3 results)

  • [Presentation] リグニンバイオマスリファイナリーの可能性2016

    • Author(s)
      梅澤俊明
    • Organizer
      JBA植物バイオ研究会第7回会合
    • Place of Presentation
      東京
    • Year and Date
      2016-04-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] p-クマロイルエステル 3-ヒドロキシラーゼ遺伝子の発現制御によるイネリグニンの構造改変2016

    • Author(s)
      武田ゆり、小柴太一、飛松裕基、村上真也、山村正臣、坂本正弘、鈴木史朗、梅澤俊明
    • Organizer
      第66回 日本木材学会
    • Place of Presentation
      名古屋
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 大型イネ科バイオマス植物エリアンサス及びサトウキビのアルカリ処理前・後におけるリグノセルロース性状解析2016

    • Author(s)
      宮本託志、山村正臣、飛松裕基、鈴木史朗、児島美穂、高部圭司、梅澤俊明
    • Organizer
      第66回 日本木材学会
    • Place of Presentation
      名古屋
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヨウ化水素酸によるリグニン由来芳香族化合物の還元的酸分解2016

    • Author(s)
      宮川泰幸、山村正臣、鈴木史朗、飛松裕基、高野俊幸、梅澤俊明
    • Organizer
      第66回 日本木材学会
    • Place of Presentation
      名古屋
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] エリアンサスおよびソルガム茎の組織分画物におけるリグノセルロースの性状解析2016

    • Author(s)
      林 晃大、山村正臣、飛松裕基、宮本託志、児嶋美穂、高部圭司、鈴木史朗、梅澤俊明
    • Organizer
      第66回 日本木材学会
    • Place of Presentation
      名古屋
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] イネリグニン生合成に関わるシンナミルアルコールデヒドロゲナーゼ遺伝子群の発現解析と変異体の性状評価2016

    • Author(s)
      松本直之、武田ゆり、飛松裕基、小柴太一、鈴木史朗、坂本正弘、梅澤俊明
    • Organizer
      第66回 日本木材学会
    • Place of Presentation
      名古屋
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Production of Biomass Energy and Material through Revegetation of Alang-alang (Imperata cylindrica) Fields2016

    • Author(s)
      Toshiaki Umezawa, Daisuke Shibata, Kenji Umemura, Masaru Kobayashi, Didik Widyatmoko, I Made Sudiana, Endang Sukara
    • Organizer
      SCEJ 81st Annual Meeting (2016) International symposium for biomass utilization and perspectives in Southeast Asia-
    • Place of Presentation
      Suita
    • Year and Date
      2016-03-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Producing Biomass Energy and Material through Revegetation of Alang-alang (Imperata cylindrica) Fields2016

    • Author(s)
      Toshiaki Umezawa, Daisuke Shibata, Kenji Umemura, Masaru Kobayashi
    • Organizer
      The 6th Flagship Symposium of Tropical Artificial Forest
    • Place of Presentation
      Uji
    • Year and Date
      2016-02-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 熱帯リグノセルロース資源の持続的生産利用2016

    • Author(s)
      梅澤俊明
    • Organizer
      植物CO2 資源化研究拠点ネットワーク(NC-CARP)産学連携コンソーシアム 第11回バイオマスリファイナリー研究会
    • Place of Presentation
      東京
    • Year and Date
      2016-01-22
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] フェルラ酸5-ヒドロキシラーゼOsF5H1の発現制御によるイネリグニンの構造改変2015

    • Author(s)
      武田ゆり、小柴太一、飛松裕基、山村正臣、服部武文、坂本正弘、高野俊幸、鈴木史朗、梅澤俊明
    • Organizer
      第60回リグニン討論会
    • Place of Presentation
      つくば
    • Year and Date
      2015-11-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] OsF5H1発現制御によるイネリグニンの芳香核組成改変2015

    • Author(s)
      武田ゆり、小柴太一、飛松裕基、山村正臣、服部武文、坂本正弘、高野俊幸、鈴木史朗、梅澤俊明
    • Organizer
      第33回日本植物細胞分子生物学会大会
    • Place of Presentation
      東京
    • Year and Date
      2015-08-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Structural modification and increase of lignin in Oryza sativa for biomass refinery2015

    • Author(s)
      Yuri Takeda1, Taichi Koshiba, Yuki Tobimatsu, Masaomi Yamamura, Masahiro Sakamoto, Toshiyuki Takano, Shiro Suzuki, Takefumi Hattori, Toshiaki Umezawa
    • Organizer
      54th Annual Meeting of the Phytochemical Society of North America (PSNA 2015)
    • Place of Presentation
      Illinois, USA
    • Year and Date
      2015-08-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イネリグニン合成パスウェイの改変2015

    • Author(s)
      梅澤俊明、奥西智哉、坂本正弘、鈴木史朗、山村正臣、飛松裕基、武田ゆり、林晃大
    • Organizer
      第288回生存圏シンポジウム、第6回 DASH/FBAS全国共同利用成果告会
    • Place of Presentation
      宇治
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] リグニンの生合成と構造特性2015

    • Author(s)
      梅澤俊明
    • Organizer
      SIPリグニン ワークショップ
    • Place of Presentation
      つくば
    • Year and Date
      2015-06-12
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] エリアンサスの化学構造特性2015

    • Author(s)
      山村正臣、梅澤俊明
    • Organizer
      第279回 生存圏シンポジウム (第5回 生存圏熱帯人工林フラッグシップシンポジウム)
    • Place of Presentation
      京都大学、京都
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] OsF5H1ノックダウンによるイネリグニン生合成の改変2015

    • Author(s)
      武田ゆり,小柴太一,飛松裕基,山村正臣,坂本正弘,高野俊幸,鈴木史朗,梅澤俊明
    • Organizer
      第65回 日本木材学会
    • Place of Presentation
      タワーホール船掘、東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大型イネ科植物エリアンサスのリグノセルロース特性における組織間の差異2015

    • Author(s)
      林 晃大,山村正臣, 高部圭司,坂本正弘,鈴木史朗,梅澤俊明
    • Organizer
      第65回 日本木材学会
    • Place of Presentation
      タワーホール船掘、東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] High-throughput protocols of lignin analysis2015

    • Author(s)
      Masaomi Yamamura, Shiro Suzuki, Takefumi Hattori and Toshiaki Umezawa
    • Organizer
      International Symposium on Wood Science and Technology 2015
    • Place of Presentation
      タワーホール船掘、東京
    • Year and Date
      2015-03-15 – 2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Lignin metabolic engineering in Oryza sativa for biomass refinering2015

    • Author(s)
      Toshiaki Umezawa, Masahiro Sakamoto, Taichi Koshiba, Takefumi Hattori, Shiro Suzuki and Masaomi Yamamura
    • Organizer
      International Symposium on Wood Science and Technology 2015
    • Place of Presentation
      タワーホール船掘、東京
    • Year and Date
      2015-03-15 – 2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 熱帯植物の持続的生産利用と植物バイオマスの高度利用に向けたリグニン代謝工学2015

    • Author(s)
      梅澤俊明
    • Organizer
      香川大学応用生命化学研究センター第6回公開シンポジウム 生命を分子レベルで探求する
    • Place of Presentation
      香川大学、香川
    • Year and Date
      2015-03-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Lignin Metabolic Engineering for Biomass Refinery2014

    • Author(s)
      Toshiaki Umezawa
    • Organizer
      Workshop on the Research for Genetically Modified Woody Plants and Biomass 2014
    • Place of Presentation
      Nanjing, China
    • Year and Date
      2014-10-17 – 2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] リグノセルロース酵素糖化に及ぼす試料加熱前処理の効果2014

    • Author(s)
      小柴太一、向井まい、服部武文、鈴木史朗、坂本正弘、梅澤俊明
    • Organizer
      第59回リグニン討論会
    • Place of Presentation
      福井工業大学、福井
    • Year and Date
      2014-09-11 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Upregulation of Lignin Biosynthesis in Oryza sativa for Biomass Refinering2014

    • Author(s)
      Taichi Koshiba, Naoki Yamamoto, Takefumi Hattori, Mai Mukai, Shiro Suzuki, Masahiro Sakamoto, Toshiaki Umezawa
    • Organizer
      ICP2014, XXVIIth International Conference on Polyphenols
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2014-09-02 – 2014-09-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] CAD2 deficiency causes both brown midrib and gold hull and internode phenotypes in Oryza sativa2014

    • Author(s)
      Taichi Koshiba, Shinya Murakami, Takefumi Hattori, Mai Mukai, Akira Takahashi, Akio Miyao, Hirohiko Hirochika, Shiro Suzuki, Masahiro Sakamoto, Toshiaki Umezawa
    • Organizer
      53rd Annual Meeting of the Phytochemical Society of North America (PSNA 2014)
    • Place of Presentation
      Raleigh, USA
    • Year and Date
      2014-08-09 – 2014-08-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Plant biotechnology towards sustainable production and utilization of tropical plant biomass resources2014

    • Author(s)
      Toshiaki Umezawa
    • Organizer
      Bandung Symposium on ASEAN University Network (AUN) - Kyoto University (KU) Student Mobility Program toward Human Security Development
    • Place of Presentation
      Bandung, Indonesia
    • Year and Date
      2014-06-24 – 2014-06-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] イネリグニン合成パスウェイの改変2014

    • Author(s)
      梅澤俊明、高辻博志、坂本正弘、鈴木史朗、山村正臣、野田壮一郎、村田久美子、安井あゆみ
    • Organizer
      第259回生存圏シンポジウム、第5回 DASH/FBAS全国共同利用成果告会
    • Place of Presentation
      京都大学、京都
    • Year and Date
      2014-06-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] イネbrown-midrib 変異体の解析2014

    • Author(s)
      小柴太一, 村上真也, 向井まい, 服部武文, 宮尾安藝雄, 廣近洋彦, 鈴木史朗, 坂本正弘, 梅澤俊明
    • Organizer
      第58回リグニン討論会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] イネ科植物細胞壁成分フェルラ酸二量体の測定のための基盤構築2014

    • Author(s)
      安井あゆみ、山村正臣、高部圭司、鈴木史朗、梅澤俊明
    • Organizer
      第64回日本木材学会大会
    • Place of Presentation
      松山
    • Related Report
      2013 Annual Research Report
  • [Presentation] 熱帯バイオマス植物の持続的生産と利用の応用展開2014

    • Author(s)
      梅澤俊明、矢﨑一史、杉山暁史、鈴木史朗、山村正臣、柴田大輔、三位正洋、我有満、上床修弘
    • Organizer
      第248回生存圏シンポジウム ミッションシンポジウム
    • Place of Presentation
      宇治
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物バイオマスの高度利用に向けたリグニン代謝工学の展望

    • Author(s)
      梅澤俊明
    • Organizer
      第28回植物バイテクシンポジウム
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] 植物細胞壁実験法2016

    • Author(s)
      梅澤俊明(石井忠、石水毅、梅澤俊明、加藤陽治、岸本崇生、小西照子、松永俊明 編)
    • Total Pages
      403
    • Publisher
      弘前大学出版会
    • Related Report
      2015 Annual Research Report
  • [Remarks] 京都大学生存圏研究所森林代謝機能化学分野

    • URL

      http://www.rish.kyoto-u.ac.jp/lmsfpm/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 京都大学生存圏研究所森林代謝機能化学分野

    • URL

      http://www.rish.kyoto-u.ac.jp/lmsfpm/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 京都大学生存圏研究所森林代謝機能化学分野

    • URL

      http://www.rish.kyoto-u.ac.jp/W/LMSFPM/

    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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