Production of kelp strains which have environment adaptability and development of the cultivation method utilizing bio-flavor

• Project/Area Number: 25292116
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Hokkaido University
• Principal Investigator: YOTSUKURA Norishige 北海道大学, 北方生物圏フィールド科学センター, 准教授 (60312344)
• Co-Investigator(Kenkyū-buntansha): HOSHINO Yoichiro 北海道大学, 北方生物圏フィールド科学センター, 准教授 (50301875) ; KUROKAWA Takayuki 北海道大学, 生命科学研究院, 准教授 (40451439)
• Co-Investigator(Renkei-kenkyūsha): KAWAI Tadashi 北海道立総合研究機構, 稚内水産試験場, 主査 (90644419)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: コンブ / バイオフレーバー / 種苗 / 環境適応株

Outline of Final Research Achievements

Gametophyte of six industrial kelp species in Hokkaido were stocked under axenic condition. Zoospores were isolated from eight sporophytes, that were produced by selection or crossing, including individuals that cultured under high water temperature and low nutrient conditions, and the germinations were cultivated. Meanwhile, cellulose was picked as an epiphytic medium for seedling of kelp. In the inspersion experiment of seedling at the field, the growth of sporophytes that were thought to be germination of the seedlings was found after the half a year of execution. Furthermore, AgNa gel was picked as a supported substrate of bio-flavor extracted from kelp. It was confirmed that aroma substances work on attracting of sea urchin in the laboratory experiment. Because efficiency of the substrates containing the substances was confirmed for capture of sea urchin at the field, the application for cultivation of seedling is expected.

Research Products

• [Journal Article] Genetic differences among varieties of Saccharina japonica in northern Japan as determined by AFLP and SSR analyses (2016)
  • Author(s): Norishige Yotsukura, Takashi Maeda, Tsuyoshi Abe, Masahiro Nakaoka, Tadashi Kawai
  • Journal Title: Journal of Applied Phycology Volume: 印刷中 Issue: 5 Pages: 3043-3055
  • DOI: 10.1007/s10811-016-0807-6
  • NAID: 120006360050
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Quantitative Observation of Electric Potential Distribution of Brittle Polyelectrolyte Hydrogels Using Microelectrode Technique (2016)
  • Author(s): Honglei Guo, Takayuki Kurokawa, Masakazu Takahata, Wei Hong, Yoshinori Katsuyama, Feng Luo, Jamil Ahmed, Tasuku Nakajima, Takayuki Nonoyama, Jian Ping Gong
  • Journal Title: Macromolecules Volume: 印刷中 Issue: 8 Pages: 3100-3108
  • DOI: 10.1021/acs.macromol.6b00037
  • NAID: 120006024346
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Assessment of genetic relationships among cultivated and wild Rubusaccessions using AFLP markers (2015)
  • Author(s): Tomoya Miyashita, Hisato Kunitake, Norishige Yotsukura, Yoichiro Hoshino
  • Journal Title: Scientia Horticulturae Volume: 193 Pages: 165-173
  • DOI: 10.1016/j.scienta.2015.07.004
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Potential resource of Saccharina bongardiana for kelp fisheries in South Kamchatka, Far East Russia (2015)
  • Author(s): Tadashi Kawai, Nobu Nagai, Nina G. Klochkoba, Norishige Yotsukura
  • Journal Title: Algal Resources Volume: 8 Pages: 59-64
  • NAID: 130007785631
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Catch and cultivation of Saccharina japonica in Northern Hokkaido, Japan, and southern Sakhalin and Primorye, Russia: A review (2015)
  • Author(s): Tadashi Kawai, Dmitrii Galanin, Tatiana N Krupnova, Norishige Yotsukura
  • Journal Title: Algal Resources Volume: 8 Pages: 155-163
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 北海道泊村沿岸表層におけるホソメコンブ遊走子の移送と空間的分布 (2015)
  • Author(s): 秋野秀樹・川井唯史・四ツ倉典滋・河野時廣
  • Journal Title: 水産工学 Volume: 52 Pages: 1-9
  • NAID: 110009976962
  • Peer Reviewed
• [Journal Article] Friction of zwitterionic hydrogel by dynamic polymer adsorption (2015)
  • Author(s): Jamil Ahmed, Tetsurou Yamamoto, Honglei Guo, Takayuki Kurokawa, Takayuki Nonoyama, Tasuku Nakajima, Jian Ping Gong
  • Journal Title: Macromolecules Volume: 48 Issue: 15 Pages: 5394-5401
  • DOI: 10.1021/acs.macromol.5b00602
  • NAID: 120005819298
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Self-Adjustable Adhesion of Polyampholyte Hydrogels (2015)
  • Author(s): Chanchal Kumar Roy, Honglei Guo, Tao Lin Sun, Abu Bin Ihsan, Takayuki Kurokawa, Masakazu Takahata, Takayuki Nonoyama, Tasuku Nakajima, Jian Ping Gong
  • Journal Title: Advanced Materials Volume: 27 Issue: 45 Pages: 7344-7348
  • DOI: 10.1002/adma.201504059
  • NAID: 120005893287
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of important marine areas around the Japanese Archipelago: Establishment of a protocol for evaluating a broad area using ecologically and biologically significant areas selection criteria. (2015)
  • Author(s): Takehisa Yamakita, Hiroyuki Yamamoto, Masahiro Nakaoka, Hiroya Yamano, Katsunori Fujikura, Kiyotaka Hidaka, Yuichi Hirota, Tadafumi Ichikawa, Shigeho Kakehi, Takahiko Kameda, Satoshi Kitajima, Kazuhiro Kogure, Teruhisa Komatsu, Naoki H. Kumagai, Hiroomi Miyamoto, Norishige Yotsukura, Yoshihisa Shirayama et al.
  • Journal Title: Marine Policy Volume: 51 Pages: 136-147
  • DOI: 10.1016/j.marpol.2014.07.009
  • Peer Reviewed / Open Access
• [Journal Article] 北海道泊村沿岸表層におけるホソメコンブ遊走子の移送と空間的分布 (2015)
  • Author(s): 秋野秀樹, 川井唯史, 四ツ倉典滋, 河野時廣
  • Journal Title: 水産工学 Volume: 印刷中
  • NAID: 110009976962
  • Peer Reviewed
• [Journal Article] Relationship between occurrence of kelp species and water temperature in northern Hokkaido, Japan and southern Sakhalin, Russia (2014)
  • Author(s): Tadashi Kawai, Dmitrii Galanin, Zhanna Tskhay, Elena Latokovskaya, Nobu Nagai, Norishige Yotsukura
  • Journal Title: Algal Resources Volume: 7 Pages: 107-116
  • NAID: 130008048010
  • Peer Reviewed
• [Journal Article] Sliding friction of zwitterionic hydrogel and its electrostatic origin (2014)
  • Author(s): Ahmed, J., Guo, H., Yamamoto, T., Kurokawa, T., Takahata, M., Nakajima, T. and Gong, J. P.
  • Journal Title: Macromolecules Volume: 47 Issue: 9 Pages: 3101-3107
  • DOI: 10.1021/ma500382y
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of CYCLOIDEA-like genes in controlling floral zygomorphy in the monocotyledon Alstroemeria (2014)
  • Author(s): Hoshino Y, Igarashi T, Ohshima M, Shinoda K,Murata N, Kanno A, Nakano M
  • Journal Title: Scientia Horticulturae Volume: 169 Pages: 6-13
  • DOI: 10.1016/j.scienta.2014.01.046
  • NAID: 120005906753
  • Peer Reviewed
• [Journal Article] Investigation of proteomic profiles of lamina of Ecklonia kurome (Laminariales): homology-based cross-species protein identification and analysis of the post-translational processing of vanadium-dependent bromoperoxidases using MALDI-TOF/TOF (2013)
  • Author(s): Kouhei Nagai, Koichi Morimoto, Haruka Ikegami, Hajime Kimura, Norishige Yotsukura
  • Journal Title: Marine Biotechnology Volume: online first Issue: 4 Pages: 487-498
  • DOI: 10.1007/s10126-013-9498-z
  • NAID: 120005460240
  • Peer Reviewed
• [Journal Article] Development of microsatellite markers for Saccharina japonica by dual-suppression PCR (2013)
  • Author(s): Takashi Maeda, Norishige Yotsukura
  • Journal Title: Algal Resources Volume: 6 Pages: 67-71
  • NAID: 130008048016
  • Peer Reviewed
• [Presentation] 北海道西日本海沿岸で持続的に形成されるホソメコンブ藻場の海洋環境について (2016)
  • Author(s): 安永倫明・秋野秀樹・秦 安史・吉田秀嗣・中多章文・三原行雄・大崎正二・四ツ倉典滋
  • Organizer: 平成28年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都港区)
  • Year and Date: 2016-03-29
• [Presentation] コンブ目重要海域の選定－その多様性・将来分布予測をもとに－ (2016)
  • Author(s): 渡辺健太郎・四ツ倉典滋・山北剛久・仲岡雅裕
  • Organizer: 日本生態学会大会第63回仙台大会
  • Place of Presentation: 仙台国際センター(宮城県仙台市)
  • Year and Date: 2016-03-26
• [Presentation] 水棲生物に学ぶ粘着表面設計 (2016)
  • Author(s): 黒川孝幸
  • Organizer: 日本化学会 第96回春季年会
  • Place of Presentation: 同志社大学京田辺キャンパス（京都府京田辺市）
  • Year and Date: 2016-03-26
• [Presentation] Adhesion Gain by Fibrous Skeleton Based on Fish Suction Pad (2016)
  • Author(s): 2.Chanchal Kumar Roy, Gento Shinohara, Daniel R. King, Tao Lin Sun, Jian Ping Gong, ○Takayuki Kurokawa
  • Organizer: International Symposium on Nature-Inspired Technology
  • Place of Presentation: Hotel ICC Convention Hall (Daejeon, Korea)
  • Year and Date: 2016-01-14
  • Int'l Joint Research
• [Presentation] ハイドロゲルの表面摩擦特性 (2015)
  • Author(s): 黒川孝幸
  • Organizer: 第64回高分子学会年次大会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-05-28
  • Invited
• [Presentation] Fisheries Biology of Saccharina bongardiana, South Kamchatka, Far-east Russia (2015)
  • Author(s): Tadashi Kawai, Anna V. Klimova, Nobu Nagai, Nina G. Klochkova, Norishige Yotsukura
  • Organizer: 日本応用藻類学会第14回大会
  • Place of Presentation: 東京海洋大学(東京都港区)
  • Year and Date: 2015-05-16
• [Presentation] Effect of electrostatic interaction on sliding friction of hydrogel (2015)
  • Author(s): Takayuki Kurokawa, Jamil Ahmed, Honglei Guo, Tetsurou Yamamoto, Masakazu Takahata, Tasuku Nakajima, Jian Ping Gong
  • Organizer: DYFP2014
  • Place of Presentation: Rolduc abbey, Kerklade (The Netherlands)
  • Year and Date: 2015-03-30
• [Presentation] 地球温暖化・海洋酸性化が亜寒帯沿岸生態系に及ぼす影響評価・予測に必要なデータ取得のためのモニタリング観測－北海道・忍路湾におけるpHの日周変動－ (2015)
  • Author(s): 髙尾信太郎, 藤井賢彦, 勝山吉徳, 鈴木光次, 四ツ倉典滋
  • Organizer: 日本海洋学会2015年度春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2015-03-24
• [Presentation] 北日本沿岸におけるコンブ類の分布：水温変化をもとにした将来予測 (2015)
  • Author(s): 渡辺健太郎, 四ツ倉典滋, 仲岡雅裕
  • Organizer: 日本生態学会第62回大会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
  • Year and Date: 2015-03-21
• [Presentation] Analysis accuracy of kelp forests thickness and spatial distribution by acoustic method (2014)
  • Author(s): Huamei Shao, Kenji Minami, Toshiaki Ohmura, Yoshikazu Fujikawa, Norishige Yotsukura, Masahiro Nakaoka, Kazushi Miyashita
  • Organizer: The Eighth Annual Meeting of Asian Fisheries Acoustics Society
  • Place of Presentation: National Kaohsiung Marine University, Kaohsiung (Taiwan)
  • Year and Date: 2014-11-19
• [Presentation] Establishment of a protocol for selecting EBSAs: using case studied in Japan (2014)
  • Author(s): Takehisa Yamakita, Hiroyuki Yamamoto, Masahiro Nakaoka, Hiroya Yamano, Katsunori Fujikura, Kiyotaka Hidaka, Yuichi Hirota, Tadafumi Ichikawa, Yoshie Jintsu-Uchifune, Shigeho Kakehi, Shingo Sakamoto, Masayoshi Sano, Kenji Sudo, Hiroya Sugisaki, Kazuaki Tadokoro, Norishige Yotsukura, Yoshihisa Shirayama et al.
  • Organizer: The World Conference on Marine Biodiversity 2014
  • Place of Presentation: Qingdao Huanghai Hotel, Qingdao (Chaina)
  • Year and Date: 2014-10-15
• [Presentation] Population genetic structure of Saccharina japonica in northern Japan (2014)
  • Author(s): Norishige Yotsukura, Takashi Maeda, Masahiro Nakaoka, Tadashi Kawai
  • Organizer: 7th Asian Pacific Phycological Forum
  • Place of Presentation: The East Lake International Conference Center, Wuhan (China)
  • Year and Date: 2014-09-21
• [Presentation] ダブルネットワークゲルをはじめとした各種機能性高分子ゲルの作製技術と特性制御及び応用展開 (2014)
  • Author(s): 黒川孝幸
  • Organizer: 情報機構 講習会
  • Place of Presentation: 品川区立総合区民会館「きゅりあん」(東京都品川区）
  • Year and Date: 2014-09-11
  • Invited
• [Presentation] 固体内部からのGraft-gelationによる高靱性ゲルの固体表面へのコーティング (2014)
  • Author(s): 塩本健悟, 中島祐, 黒川孝幸, Jian Ping Gong
  • Organizer: 29th Summer University in Hokkaido・2014 年度北海道高分子若手研究会
  • Place of Presentation: 定山渓ビューホテル（北海道札幌市南区）
  • Year and Date: 2014-08-29
• [Presentation] 再生医療に応用可能な機能性ゲルの創製 (2014)
  • Author(s): 黒川孝幸
  • Organizer: 第93回ニューフロンティア材料部会例会
  • Place of Presentation: 大阪市立工業研究所（大阪府大阪市城東区)
  • Year and Date: 2014-07-29
  • Invited
• [Presentation] Relationship between occurrence of kelp species and water temperature in northern Hokkaido, Japan and southern Sakhalin, Russia (2014)
  • Author(s): Tadashi Kawai, Dmitrii Galanin, Zhanna Tskhay, Elena Latokovskaya, Nobu NagaiI, Norishige Yotsukura
  • Organizer: 日本応用藻類学会第13回大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2014-05-31
• [Presentation] Flavor Chemistry for the Selection of Algal Biotechnology Material to Produce Bioflavor from Edible Kelp, Saccharina spp. (2014)
  • Author(s): Kangsadan Boonprab, Norishige Yotsukura, Yoshinori Katsuyama, Yusuke Takata and Tadahiko Kajiwara
  • Organizer: 1st Joint ACS AGFD-ACS ICSCT Symposium on Agricultural and Food Chemistry
  • Place of Presentation: Montien Riverside Hotel (Thailand)
• [Presentation] 新規“From-on”法を用いた高靱性DNゲルの固体表面コーティング (2014)
  • Author(s): 塩本健悟、岡崎春香、中島祐、黒川孝幸、グン剣萍
  • Organizer: 2014年帝人21世紀フォーラム
  • Place of Presentation: 帝人富士教育研修所（静岡県）
• [Presentation] An approach to pinpoint the geographic origin of samples of Saccharina japonica by DNA fingerprinting
  • Author(s): Maeda T, Kawai T, Nakaoka M, Yotsukura N
  • Organizer: 21st International Seaweeds Symposium
  • Place of Presentation: Bali Nusa Dua Convention Center (Indonesia)
• [Presentation] Technology for quality evaluation of raw brown seaweed
  • Author(s): Kinoshita Y, Nogami T, Yotsukura N
  • Organizer: 21st International Seaweeds Symposium
  • Place of Presentation: Bali Nusa Dua Convention Center (Indonesia)
• [Presentation] Present situation and assignment of biodiversity research on kelp in Japan
  • Author(s): Norishige Yotsukura
  • Organizer: An International Research Meeting: Front of Diversity Research on Kelp in the North Pacific
  • Place of Presentation: Hokkaido University (Hokkaido)
  • Invited
• [Presentation] Technology for quality evaluation of raw brown seaweeds
  • Author(s): Kinoshita Yasunori, Imamura Tamami, Yotsukura Norishige
  • Organizer: 1st Annual EAFTA (East Asia Fisheries Technologists Association) Conference
  • Place of Presentation: Hokkaido University (Hokkaido)
• [Presentation] 強度DNゲルによる固体基板のコーティングとその最適条件の探究
  • Author(s): 岡崎春香、中島 祐、黒川孝幸、グン剣萍
  • Organizer: 第62回高分子学会年次大会
  • Place of Presentation: 京都国際会館（京都府）
• [Presentation] 高強度DNゲルによる固体基板のコーティングの最適条件追求
  • Author(s): 岡崎春香、中島祐、黒川孝幸、グン剣萍
  • Organizer: 第48回北海道支部冬季研究発表会
  • Place of Presentation: 北海道大学(北海道）
• [Presentation] ゲル-固体間の強靭な接着を目指した、重合開始点を含有する基板からのゲル重合
  • Author(s): 塩本健悟、岡崎春香、中島祐、黒川孝幸、グン剣萍
  • Organizer: 化学系学協会北海道支部2014年冬季研究発表会
  • Place of Presentation: 北海道大学(北海道）
• [Book] 北海道つながる海と川の生き物 (2015)
  • Author(s): 川井唯史, 四ツ倉典滋, 富安俊, 幡宮輝雄, 藤森英樹, 武田康孝, 野本和宏, 中原修, 野手啓行
  • Total Pages: 190
  • Publisher: 北海道新聞社