| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
The objective of this study is development of artificial antibody using the nucleotide sequence encoding variable region of fish immunoglobulin genes. Antibody responses in Japanese flounder, rainbow trout and bester sturgeon were measured before and after immunization with various antigens, including pathogenic bacteria and certain proteins. In Japanese flounder and rainbow trout, specific antibody titers were increased after the immunization, while the leukocytes expressing specific antibody were not isolated. In contrast, bester sturgeon possessed specific antibodies against various antigen without immunization. We isolated cDNA from a single sturgeon blood leukocyte, which bound to the fluorescent labeled bacterial cells, and amplified the IgM heavy chain gene. This technique will allow us to develop artificial fish monoclonal antibodies.
In addition, we identified a number of genes involved in immunity and specific antibody responses.
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