• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanism of activation and regulation of immune response by thrombocytes in fish

Research Project

Project/Area Number 25292127
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionKyushu University

Principal Investigator

Nakao Miki  九州大学, (連合)農学研究科(研究院), 教授 (50212080)

Co-Investigator(Kenkyū-buntansha) 杣本 智軌  九州大学, (連合)農学研究科(研究院), 准教授 (40403993)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywords魚類 / 栓球 / 免疫 / 血液凝固 / 白血球 / 食細胞 / 貪食活性 / 食作用 / コイ / 補体
Outline of Final Research Achievements

Thrombocytes are regarded as the functional equivalent of anucleated mammalian platelets. Additional immune functions, including phagocytosis, have also been suggested, but no conclusive experimental evidence for their potential ingestion and clearance of infiltrating microbes has been provided till date. Here we demonstrate the active phagocytic ability of thrombocytes in lower vertebrates using teleost fishes and amphibian models. Carp thrombocytes were able to ingest live bacteria and kill them. We found that thrombocytes represented nearly half of the phagocyte population in the common carp total peripheral blood leukocyte pool. Phagocytosis efficiency was further enhanced by serum opsonization. Particle internalization led to phagolysosome fusion and killing of internalized bacteria, pointing to a robust ability for microbe elimination. We find that this potent phagocytic activity is shared across fish and frogs, implying its conservation throughout the lower vertebrate lineage.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (21 results)

All 2016 2015 2014 2013

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Functional analysis of membrane-bound complement regulatory protein on T-cell immune response in ginbuna crucian carp2016

    • Author(s)
      Nur I, Abdelkhalek NK, Motobe S, Nakamura R, Tsujikura M, Somamoto T, Nakao M.
    • Journal Title

      Molecular Immunology

      Volume: 70 Pages: 1-7

    • DOI

      10.1016/j.molimm.2015.11.010

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Carp thrombocyte phagocytosis requires activation factors secreted from other leukocytes2015

    • Author(s)
      Nagasawa T, Somamoto T, Nakao M
    • Journal Title

      Developmental and Comparative Immunology

      Volume: 52 Issue: 2 Pages: 107-111

    • DOI

      10.1016/j.dci.2015.05.002

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Local and systemic adaptive immune responses toward viral infection via gills in ginbuna crucian carp2015

    • Author(s)
      Somamoto T, Miura Y, Nakanishi T, Nakao M.
    • Journal Title

      Developmental and Comparative Immunology

      Volume: 52 Issue: 1 Pages: 81-87

    • DOI

      10.1016/j.dci.2015.04.016

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Ulcer disease prophylaxis in koi carp by bath immersion with chicken egg yolk containing anti-Aeromonas salmonicida IgY.2015

    • Author(s)
      Gan H, He H, Sato A, Hatta H, Nakao M, Somamoto T.
    • Journal Title

      Research in Veterinary Science

      Volume: 99 Pages: 82-86

    • DOI

      10.1016/j.rvsc.2015.01.016

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A CD46-like molecule functional in teleost fish represents an ancestral form of membrane-bound regulators of complement activation2015

    • Author(s)
      Tsujikura M, Nagasawa T, Ichiki S, Nakamura R, Somamoto T, Nakao M.
    • Journal Title

      J. Immunol.

      Volume: 194 Issue: 1 Pages: 262-272

    • DOI

      10.4049/jimmunol.1303179

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Phagocytosis by Thrombocytes is a Conserved Innate Immune Mechanism in Lower Vertebrates.2014

    • Author(s)
      Nagasawa T, Nakayasu C, Rieger AM, Barreda DR, Somamoto T, Nakao M
    • Journal Title

      Front. Immunol.

      Volume: 5 Pages: 445-445

    • DOI

      10.3389/fimmu.2014.00445

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Helper function of CD4⁺ lymphocytes in antiviral immunity in ginbuna crucian carp, Carassius auratus langsdorfii.2014

    • Author(s)
      Somamoto T, Kondo M, Nakanishi T, Nakao M.
    • Journal Title

      Dev Comp Immunol.

      Volume: 44 Issue: 1 Pages: 111-115

    • DOI

      10.1016/j.dci.2013.12.008

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Hagfish C1q: its unique binding property2014

    • Author(s)
      Yamaguchi T, Takamune K, Kondo M, Takahashi Y, Kato-Unoki Y, Nakao M, Sano N, Fujii T.
    • Journal Title

      Dev Comp Immunol

      Volume: 43 Issue: 1 Pages: 47-53

    • DOI

      10.1016/j.dci.2013.10.009

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and functional characterization of complement C3 and a novel zymosan-binding protein in tilapia serum2014

    • Author(s)
      Abdel-Salem SGR, Tsujikura M, Kondo M, Somamoto T, Nakao M
    • Journal Title

      Fisheries Sciences

      Volume: 80 Issue: 2 Pages: 301-310

    • DOI

      10.1007/s12562-014-0700-7

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization and expression analysis of three membrane-bound complement regulatory protein isoforms in the ginbuna crucian carp Carassius auratus langsdorfii2013

    • Author(s)
      Nur I, Harada H, Tsujikura M, Somamoto T, Nakao M
    • Journal Title

      Fish Shellfish Immunol

      Volume: 35 Issue: 4 Pages: 1333-1337

    • DOI

      10.1016/j.fsi.2013.08.002

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Expression and homeostatic functions of Tecrem, a CD46-like complement regulatory protein, on epithelial cells in bony fish2016

    • Author(s)
      Prakash H, Motobe S, Nagasawa T, Somamoto T, Nakao M
    • Organizer
      International Complement Workshop
    • Place of Presentation
      Hotel Nikko Kanazawa
    • Year and Date
      2016-09-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Carp properdin: structural and functional diversity of two isotypes2016

    • Author(s)
      Yoshioka K, Kato-Unoki Y, Nagasawa T, Somamoto T, Nakao M
    • Organizer
      International Complement Workshop
    • Place of Presentation
      Hotel Nikko Kanazawa
    • Year and Date
      2016-09-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 魚類の補体系における成分の多重化とその機能的意義2015

    • Author(s)
      中尾実樹
    • Organizer
      第27回日本比較免疫学会
    • Place of Presentation
      福井県立大学
    • Year and Date
      2015-08-21
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Structural and functional diversity of properdin isotypes in the common carp complement system.2015

    • Author(s)
      Yoshioka K, Kato-Unoki Y, Somamoto T, Nakao M
    • Organizer
      13th Congress of the International Society for Developmental and Comparative Immunology
    • Place of Presentation
      Murcia, Spain
    • Year and Date
      2015-06-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CD46様コイ補体制御因子(Tecrem)の上皮細胞における表面発現動態2014

    • Author(s)
      元部詩織、辻倉正和、中村亮太、杣本智軌、中尾実樹
    • Organizer
      日本補体学会
    • Place of Presentation
      神戸常盤大学
    • Year and Date
      2014-08-22 – 2014-08-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] コイ補体Properdinアイソフォームの多様性と機能解析2014

    • Author(s)
      吉岡和紀、鵜木陽子、杣本智軌、中尾実樹
    • Organizer
      日本比較免疫学会
    • Place of Presentation
      東北大学
    • Year and Date
      2014-07-09 – 2014-07-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 魚類栓球の異物分解・殺菌作用2014

    • Author(s)
      長澤貴宏, 中易千早, Aja M. Rieger, Daniel R. Barreda, 杣本 智軌, 中尾 実樹
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2014-04-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] 魚類栓球の異物分解・殺菌作用2014

    • Author(s)
      長沢貴宏、中易千早、Rieger AM、Barreda DR、杣本智軌、中尾実樹
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 不活化ウイルスの経腸管感作によって誘導されるギンブナT最御坊の免疫応答及び感染防御効果2013

    • Author(s)
      多治見誠亮、杣本智軌、中西照幸、中尾実樹
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      三重大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Characterization of membrane-bound regulatory protein in ginbuna crucian carp Carassius auratus langsdorfii2013

    • Author(s)
      Nur I, Harada H, Nakamura R, Tsujikura M, Somamoto T, Nakao M
    • Organizer
      日本比較免疫学会学術集会
    • Place of Presentation
      岡山理科大学
    • Related Report
      2013 Annual Research Report
  • [Book] The evolution of complement system functions and pathways in vertebrates. in The evolution of the immune system: a balance between conservation and diversity2016

    • Author(s)
      Nakao M, Somamoto T
    • Total Pages
      25
    • Publisher
      Elsevier
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi