Molecular mechanism of activation and regulation of immune response by thrombocytes in fish
Project/Area Number |
25292127
|
Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Aquatic life science
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Research Institution | Kyushu University |
Principal Investigator |
Nakao Miki 九州大学, (連合)農学研究科(研究院), 教授 (50212080)
|
Co-Investigator(Kenkyū-buntansha) |
杣本 智軌 九州大学, (連合)農学研究科(研究院), 准教授 (40403993)
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
|
Keywords | 魚類 / 栓球 / 免疫 / 血液凝固 / 白血球 / 食細胞 / 貪食活性 / 食作用 / コイ / 補体 |
Outline of Final Research Achievements |
Thrombocytes are regarded as the functional equivalent of anucleated mammalian platelets. Additional immune functions, including phagocytosis, have also been suggested, but no conclusive experimental evidence for their potential ingestion and clearance of infiltrating microbes has been provided till date. Here we demonstrate the active phagocytic ability of thrombocytes in lower vertebrates using teleost fishes and amphibian models. Carp thrombocytes were able to ingest live bacteria and kill them. We found that thrombocytes represented nearly half of the phagocyte population in the common carp total peripheral blood leukocyte pool. Phagocytosis efficiency was further enhanced by serum opsonization. Particle internalization led to phagolysosome fusion and killing of internalized bacteria, pointing to a robust ability for microbe elimination. We find that this potent phagocytic activity is shared across fish and frogs, implying its conservation throughout the lower vertebrate lineage.
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Report
(5 results)
Research Products
(21 results)
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[Presentation] 魚類栓球の異物分解・殺菌作用2014
Author(s)
長澤貴宏, 中易千早, Aja M. Rieger, Daniel R. Barreda, 杣本 智軌, 中尾 実樹
Organizer
平成26年度日本水産学会春季大会
Place of Presentation
東京海洋大学
Year and Date
2014-04-01
Related Report
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