Development of techniques for prevention of fish disease by the analysis of IFNgamma function in fish immune response

• Project/Area Number: 25292129
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Nihon University
• Principal Investigator: NAKANISHI Teruyuki 日本大学, 生物資源科学部, 教授 (00322496)
• Co-Investigator(Kenkyū-buntansha): MANO Nobuhiro 日本大学, 生物資源科学部, 准教授 (10339286) ; SOMAMOTO Tomonori 九州大学, 農学研究科, 准教授 (40403993) ; ARAKI Kyousuke 鹿児島大学, 水産学部, 助教 (30409073) ; TAKANO Tomokazu 国立研究開発法人水産総合研究センター, その他部局等, 研究員 (40533998) ; NAKAYASU Chihaya 独立行政法人水産総合研究センター, その他部局等, 研究員 (00311225)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 魚類 / インターフェロンγ / 細胞性免疫 / 細胞内寄生細菌 / クローンギンブナ / 抗酸菌 / T細胞 / IFNγ

Outline of Final Research Achievements

In this project, we showed a) binding of fish specific recombinant IFNγ, rgIFNγrel 1 to class II cytokine receptor family member, Crfb5 and Crfb17 and activation of STAT6, b) important roles of fish IFNγ and IFNγrel in the protection against infection caused by virus and cell-associated bacteria, c) increase in the number of neutrophils/macrophages and the decrease in mortality after E. tarda challenge after rgIFNγ 2 administration, d) enhanced allograft rejection and the increase in the number of T cells and sIgM+ cells in scale allografts by rgIFNγrel 1 administration, e) enhanced potency of vaccines after rgIFNγ administration.

Research Products

• [Journal Article] Recombinant carp IL-4/13B stimulates in vitro proliferation of carp IgM+ B cells (2016)
  • Author(s): Takuya Yamaguchi, Shunsuke Miyata, Fumihiko Katakura, Takahiro Nagasawa, Yasuhiro Shibasaki, Takeshi Yabu, Uwe Fischer, Chihaya Nakayasu, Teruyuki Nakanishi, Tadaaki Moritomo
  • Journal Title: Fish & Shellfish Immunology Volume: 49 Pages: 225-229
  • DOI: 10.1016/j.fsi.2015.12.043
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Sequential steps of macroautophagy and chaperone-mediated autophagy are involved in the irreversible process of posterior silk gland histolysis during metamorphosis of Bombyx mori. (2016)
  • Author(s): Shiba, H., T. Yabu, M. Sudayama, N. Mano, N. Arai, T. Nakanishi, and K. Hosono
  • Journal Title: J. Exp. Biol. Volume: 1 Pages: 1-10
  • DOI: 10.1242/jeb.130815
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional analysis of membrane-bound complement regulatory protein on T-cell immune response in ginbuna crucian carp (2016)
  • Author(s): Nur I, Abdelkhalek NK, Motobe S, Nakamura R, Tsujikura M, Somamoto T, Nakao M.
  • Journal Title: Molecular Immunology Volume: 70 Pages: 1-7
  • DOI: 10.1016/j.molimm.2015.11.010
  • Peer Reviewed
• [Journal Article] Purification and characterization of a fish granzymeA involved in cell-mediated immunity. (2016)
  • Author(s): Matsuura Y, T. Yabu, H. Shiba, T. Moritomo, T. Nakanishi
  • Journal Title: Dev Comp Immunol. Volume: 60 Pages: 33-40
  • DOI: 10.1016/j.dci.2016.02.011
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Kinetics of lymphocyte subpopulations in allogeneic grafted scales of ginbuna crucian carp (2015)
  • Author(s): Shibasaki Y., Matsuura Y., Toda H., Imabayashi N., Nishino T., Uzumaki K., Hatanaka C.,Yabu T., Moritomo T, NakanishiT.,
  • Journal Title: Dev Comp Immunol Volume: 52 Issue: 1 Pages: 75-80
  • DOI: 10.1016/j.dci.2015.04.013
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Local and systemic adaptive immune responses toward viral infection via gills in ginbuna crucian carp (2015)
  • Author(s): Somamoto T, Miura Y, Nakanishi T, Nakao M.
  • Journal Title: Developmental and Comparative Immunology Volume: 52 Issue: 1 Pages: 81-87
  • DOI: 10.1016/j.dci.2015.04.016
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Comparative analysis of adaptive immune response after vaccine trials using live attenuated and formalin-killed cells of Edwardsiella tarda in ginbuna crucian carp (Carassius auratus langsdorfii) (2015)
  • Author(s): Yamasaki, M., K. Araki, K. Maruyoshi, M. Matsumoto, C. Nakayasu, T. Moritomo, T. Nakanishi, A. Yamamoto
  • Journal Title: Fish and Shellfish Immunology Volume: 45 Issue: 2 Pages: 437-442
  • DOI: 10.1016/j.fsi.2015.04.038
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] In vitro characteristics of cyprinid herpesvirus 2: effect of kidney extract supplementation on growth (2015)
  • Author(s): T Shibata, A Nanjo, M Saito, K Yoshii, T Ito, T Nakanishi, T Sakamoto, M Sano
  • Journal Title: Diseases of Aquatic Organisms Volume: 115 Issue: 3 Pages: 223-232
  • DOI: 10.3354/dao02885
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Exploring erythropoiesis of common carp (Cyprinus carpio) using an in vitro colony assay in the presence of recombinant carp kit ligand A and erythropoietin (2015)
  • Author(s): Fumihiko Katakura, Takeshi Yabu, Takuya Yamaguchi, Jiro Miyamae, Yuki Shirinashihama, Teruyuki Nakanishi, Tadaaki Moritomo
  • Journal Title: Developmental and Comparative Immunology Volume: 53 Issue: 1 Pages: 13-22
  • DOI: 10.1016/j.dci.2015.06.006
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] T cells in fish (2015)
  • Author(s): Nakanishi T., Shibasaki Y., Y. Matsuura
  • Journal Title: Biology Volume: 4 Issue: 4 Pages: 640-663
  • DOI: 10.3390/biology4040640
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Carp thrombocyte phagocytosis requires activation factors secreted from other leukocytes (2015)
  • Author(s): Nagasawa T, Somamoto T, Nakao M
  • Journal Title: Developmental and Comparative Immunology Volume: 52 Issue: 2 Pages: 107-111
  • DOI: 10.1016/j.dci.2015.05.002
  • Peer Reviewed
• [Journal Article] Recombinant sialidase NanA (rNanA) cleaves alpha2-3 linked sialic acid of host cell surface N-linked glycoprotein to promote Edwardsiella tarda infection (2015)
  • Author(s): Chigwechokha, P.K., M. Tabata, S. Shinyoshi, K. Oishi, K. Araki, M. Komatsu, T. Itakura, K. Shiozaki
  • Journal Title: Fish and Shellfish Immunology Volume: 47 Issue: 1 Pages: 34-45
  • DOI: 10.1016/j.fsi.2015.08.015
  • Peer Reviewed
• [Journal Article] A CD46-like molecule functional in teleost fish represents an ancestral form of membrane-bound regulators of complement activation (2015)
  • Author(s): Tsujikura M, Nagasawa T, Ichiki S, Nakamura R, Somamoto T, Nakao M.
  • Journal Title: J. Immunol. Volume: 194 Issue: 1 Pages: 262-272
  • DOI: 10.4049/jimmunol.1303179
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Stress-induced ceramide generation and apoptosis via the phosphorylation and activation of nSMase1 by JNK signaling. (2015)
  • Author(s): Yabu T, Shiba H, Shibasaki Y, Nakanishi T, Imamura S, Touhata K, Yamashita M.
  • Journal Title: Cell Death Differ. Volume: 22 Issue: 2 Pages: 258-273
  • DOI: 10.1038/cdd.2014.128
  • Peer Reviewed
• [Journal Article] Ulcer disease prophylaxis in koi carp by bath immersion with chicken egg yolk containing anti-Aeromonas salmonicida IgY. (2015)
  • Author(s): Gan H, He H, Sato A, Hatta H, Nakao M, Somamoto T.
  • Journal Title: Research in Veterinary Science Volume: 99 Pages: 82-86
  • DOI: 10.1016/j.rvsc.2015.01.016
  • Peer Reviewed
• [Journal Article] Easy-to-use rapid gene amplification method for direct detection of RNA and DNA viruses in sera and feces from various animals (2014)
  • Author(s): Segawa T, Kobayashi Y, Sase Y, Itou T, Suzuki M, Endoh T, Nakanishi T, Sakai
  • Journal Title: J Virol Methods. Volume: 201C Pages: 31-37
  • DOI: 10.1016/j.jviromet.2014.01.019
  • Peer Reviewed
• [Journal Article] Lack of a contact requirement for direct antibacterial activity of lymphocyte subpopulations in ginbuna crucian carp. (2014)
  • Author(s): Tartor, H. M., Matsuura Y., El-Nobi G., Nakanishi, T.
  • Journal Title: Fish Shellfish Immunol. Volume: 39 Issue: 2 Pages: 178-184
  • DOI: 10.1016/j.fsi.2014.05.006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Immune responses to live and inactivated Nocardia seriolae and protective effect of recombinant interferon gamma (rIFN γ) against nocardiosis in ginbuna crucian carp, Carassius auratus langsdorfii. (2014)
  • Author(s): Nayak S K, Y. Shibasaki and Nakanishi T.
  • Journal Title: Fish Shellfish Immunol. Volume: 39 Issue: 2 Pages: 354-364
  • DOI: 10.1016/j.fsi.2014.05.015
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of a novel fish granzyme involved in cell-mediated immunity. (2014)
  • Author(s): Matsuura, Y., Yabu, T., Shiba, H., Moritomo, T., and Nakanishi, T.
  • Journal Title: Developmental & Comparative Immunology Volume: 46 Issue: 2 Pages: 499-507
  • DOI: 10.1016/j.dci.2014.06.006
  • Peer Reviewed / Open Access
• [Journal Article] Phagocytosis by Thrombocytes is a Conserved Innate Immune Mechanism in Lower Vertebrates. (2014)
  • Author(s): Nagasawa T, Nakayasu C, Rieger AM, Barreda DR, Somamoto T, Nakao M
  • Journal Title: Front. Immunol. Volume: 5 Pages: 445-445
  • DOI: 10.3389/fimmu.2014.00445
  • Peer Reviewed / Open Access
• [Journal Article] Peculiar monomeric interferon gammas, IFNγrel 1 and IFNγrel 2, in ginb una crucian carp. (2014)
  • Author(s): Shibasaki Y., Yabu T., Araki K., Manol N., Shiba H., Moritomo T, Nakanishi T.
  • Journal Title: FEBS Journal Volume: 281 Issue: 4 Pages: 1046-1056
  • DOI: 10.1111/febs.12666
  • Peer Reviewed / Open Access
• [Journal Article] Helper function of CD4⁺ lymphocytes in antiviral immunity in ginbuna crucian carp, Carassius auratus langsdorfii. (2014)
  • Author(s): Somamoto T, Kondo M, Nakanishi T, Nakao M.
  • Journal Title: Dev Comp Immunol. Volume: 44 Issue: 1 Pages: 111-115
  • DOI: 10.1016/j.dci.2013.12.008
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Role of CD4+ and CD8α+ T cells in protective immunity against (2014)
  • Author(s): Yamasaki M, Araki K, Nakanishi T, Nakayasu C, Yamamoto A.
  • Journal Title: Fish Shellfish Immunol. Volume: 36 Issue: 1 Pages: 299-304
  • DOI: 10.1016/j.fsi.2013.11.016
  • Peer Reviewed
• [Journal Article] Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species. (2014)
  • Author(s): Takizawa, F., Araki, K., Ohtani, M., Toda, H., Saito, Y., Lampe, V.S., Dijkstra, J.M., Ototake, M., Moritomo, T., and Nakanishi, T.
  • Journal Title: Fish & shellfish immunology Volume: 36 Issue: 1 Pages: 215-222
  • DOI: 10.1016/j.fsi.2013.11.004
  • Peer Reviewed / Open Access
• [Journal Article] Antiviral functions of CD8+ cytotoxic T cells in teleost fish. (2014)
  • Author(s): Somamoto T., Koppang E.O., Fischer U.
  • Journal Title: Dev. Comp. Immunol. Volume: 43 Issue: 2 Pages: 197-204
  • DOI: 10.1016/j.dci.2013.07.014
  • Peer Reviewed
• [Journal Article] Identification of immune relevant genes using expressed sequence tags (ESTs) in common carp (Cyprinus carpio) gills and intestine. (2014)
  • Author(s): Abdelkhalek NK, Komiya A, Kato-Unoki Y, Somamoto T, Nakao M.
  • Journal Title: Journal of Faculty of Agriculture, Kyushu University. Volume: 59 Pages: 109-116
  • NAID: 120005411071
• [Journal Article] Efficacy of high-concentration ascorbic acid supplementation against (2013)
  • Author(s): Ishikawa T., N. Mano, K. Minakami, A. Namba, T. Kojima, H. Hirose and T. Nakanishi
  • Journal Title: Fish Pathology Volume: 48 Pages: 113-118
  • Peer Reviewed
• [Journal Article] Molecular characterization and expression analysis of three membrane-bound complement regulatory protein isoforms in the ginbuna crucian carp Carassius auratus langsdorfii (2013)
  • Author(s): Nur I, Harada H, Tsujikura M, Somamoto T, Nakao M
  • Journal Title: Fish Shellfish Immunol Volume: 35 Issue: 4 Pages: 1333-1337
  • DOI: 10.1016/j.fsi.2013.08.002
  • Peer Reviewed
• [Journal Article] Teleost T and NK cell immunity (2013)
  • Author(s): Fischer U., Koppang E. O. and T. Nakanishi
  • Journal Title: Fish Shellfish Immunol Volume: 35 Issue: 2 Pages: 197-206
  • DOI: 10.1016/j.fsi.2013.04.018
  • Peer Reviewed
• [Journal Article] Nakanishi Expression profiles of interferon gamma genes in response to immune-stimulants and alloantigen in ginbuna crucian carp Carassius auratus langsdorfii (2013)
  • Author(s): Araki, K., F. Takizawa, M. Yamasaki, M. Esumi, T. Moritomo, M. Ototake, A. Yamamoto, T
  • Journal Title: Fisheries Science Volume: 79巻 Issue: 2 Pages: 213-220
  • DOI: 10.1007/s12562-012-0590-5
  • Peer Reviewed
• [Journal Article] Serum GlucNAc-binding IgM of fugu (Takifugu rubripes) suppresses (2013)
  • Author(s): Tsutsui, S., Ariji, T., Sato, A., Yoshida, T., Yamamura, N., Odaka, T., Araki, K., Suetake, H., Miyadai, T., Nakamura, O.
  • Journal Title: Dev. Comp. Immunol. Volume: 41 Issue: 1 Pages: 20-26
  • DOI: 10.1016/j.dci.2013.03.018
  • Peer Reviewed
• [Journal Article] Adaptive immune response to Edwardsiella tarda infection in ginbuna crucian carp (2013)
  • Author(s): Yamasaki, M., K. Araki, T. Nakanishi, C. Nakayasu, Y. Yoshiura, T. Iida, A. Yamamoto
  • Journal Title: Carassius auratus langsdorfii. Veterinary Immunology and Immunopathology Volume: 153巻 Issue: 1-2 Pages: 83-90
  • DOI: 10.1016/j.vetimm.2013.02.004
  • Peer Reviewed
• [Journal Article] Identification of anti-viral cytotoxic effector cells in the ginbuna crucian carp Carassius auratus langsdorfii. (2013)
  • Author(s): Somamoto T, Nakanishi T and Nakao M.
  • Journal Title: Dev. Comp. Immunol. Volume: 39 Issue: 4 Pages: 370-377
  • DOI: 10.1016/j.dci.2012.11.001
  • Peer Reviewed
• [Presentation] Effects of IFNγ administration on allograft rejection in ginbuna crucian carp. (2016)
  • Author(s): Shibasaki Y., C. Hatanaka, Y. Matsuura, R. Miyazawa, T. Yabu, T. Nakanishi
  • Organizer: 2nd International Conference of Fish & Shellfish Immunology
  • Place of Presentation: ポートランド市（米国・メイン州）
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] カンパチのInterleukin-10による細胞性免疫の抑制 (2016)
  • Author(s): 松本 萌、Mahmoud Tanekhy Amer、早志和真、西谷 篤、今岡慶明、柳 宗悦、荒木亨介、山本 淳
  • Organizer: 平成28年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス(東京都港区）
  • Year and Date: 2016-03-26
• [Presentation] 魚類ミコバクテリア症原因細菌が産生するESAT-6およびCFP-10の細胞性免疫誘導能の解析 (2016)
  • Author(s): 早志和真、松本 萌、中西照幸、荒木亨介、山本 淳
  • Organizer: 平成28年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス(東京都港区）
  • Year and Date: 2016-03-26
• [Presentation] 近年日本国内で分離されたIHNVの遺伝的多様性 (2016)
  • Author(s): 難波亜紀・水上海・武江太郎・辻あすみ・岩下誠・間野伸宏・中西照幸
  • Organizer: 平成27年度日本魚病学会春季大会
  • Place of Presentation: 日本獣医生命科学大学（東京都武蔵野市）
  • Year and Date: 2016-03-12
• [Presentation] Cyprinid herpesvirus 2感染耐過魚血清を用いた受動免疫の効果 (2016)
  • Author(s): 南條 梓・柴田智也・吉井啓亮・田中深貴男・中西照幸・加藤豪司・佐野元彦
  • Organizer: 平成27年度日本魚病学会春季大会
  • Place of Presentation: 日本獣医生命科学大学（東京都武蔵野市）
  • Year and Date: 2016-03-12
• [Presentation] ギンブナのツベルクリン様反応におけるIFN-γ相同遺伝子の発現解析 (2016)
  • Author(s): 嘉数泰稚・中西照幸・佐野元彦・加藤豪司
  • Organizer: 平成27年度日本魚病学会春季大会
  • Place of Presentation: 日本獣医生命科学大学（東京都武蔵野市）
  • Year and Date: 2016-03-12
• [Presentation] 魚類細胞性免疫に関与する細胞傷害関連プロテアーゼの同定 (2015)
  • Author(s): 松浦雄太 薮健史 柴崎康宏 司馬肇 中西照幸
  • Organizer: 第38回日本分子生物学会・第88回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-03
• [Presentation] 魚類新規インターフェロン、IFNγrel 1の作用機序解明 (2015)
  • Author(s): 柴﨑康宏，松浦雄太，藪健史，中西照幸
  • Organizer: 第38回日本分子生物学会・第88回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-03
• [Presentation] E.tardaの感染予防を目的としたシアリダーゼNanA阻害物質の探索 (2015)
  • Author(s): 新吉沙弥香、若松麗菜、Petros Kingstone Chigwechokha、松崎光輝、荒木亨介、小松正治、塩崎一弘
  • Organizer: 平成27年度日本水産学会九州支部大会
  • Place of Presentation: 宮崎大学農学部（宮崎県宮崎市）
  • Year and Date: 2015-11-07
• [Presentation] ブリ類のInterferon-γ遺伝子の単離および機能解析 (2015)
  • Author(s): 松本 萌、荒木亨介、早志和真、下野友未、劉 倩、柳 宗悦、今岡慶明、山本 淳
  • Organizer: 平成27年度日本水産学会九州支部大会
  • Place of Presentation: 宮崎大学農学部（宮崎県宮崎市）
  • Year and Date: 2015-11-07
• [Presentation] Current diversity of infectious hematopoietic necrosis virus (IHNV) in Japan (2015)
  • Author(s): NambaA., K. Minakami, T. Takee, N. Mano, T. Nakanishi
  • Organizer: BioMicroWorld 2015
  • Place of Presentation: Barcelona, スペイン
  • Year and Date: 2015-10-28
  • Int'l Joint Research
• [Presentation] ブリ類の抗酸菌症不活化ワクチン接種後の免疫応答 (2015)
  • Author(s): 荒木亨介、下野友未、丸吉浩太、松本 萌、早志和真、山崎雅俊、柳 宗悦、今岡慶明、山本 淳
  • Organizer: 平成27年度日本魚病学会秋季大会
  • Place of Presentation: 東京大学弥生講堂（東京都文京区）
  • Year and Date: 2015-09-25
• [Presentation] カンパチのノカルジア症不活化ワクチンにおけるInterleukin-12のアジュバント効果 (2015)
  • Author(s): 松本 萌、荒木亨介、丸吉浩太、早志和真、末武弘章、柳 宗悦、今岡慶明、山本 淳
  • Organizer: 平成27年度日本魚病学会秋季大会
  • Place of Presentation: 東京大学弥生講堂（東京都文京区）
  • Year and Date: 2015-09-25
• [Presentation] Edwardsiella tarda感染における宿主細胞のN型糖タンパク糖鎖構造変化の重要性 (2015)
  • Author(s): 塩崎一弘、Petros Chigwechokha、新吉沙弥香、大木麗菜、荒木亨介、小松正治
  • Organizer: 平成27年度日本水産学会秋季大会
  • Place of Presentation: 東北大学（宮城県仙台市）
  • Year and Date: 2015-09-22
• [Presentation] 抗ギンブナリンパ球モノクローナル抗体によるゼブラフィッシュCD4及びCD8α陽性細胞の同定 (2015)
  • Author(s): 宮澤龍一郎, 松浦雄太, 柴崎康宏, 藪健史, 中西照幸
  • Organizer: 第21回小型魚類研究会
  • Place of Presentation: 大阪大学銀杏会館(大阪府吹田市)
  • Year and Date: 2015-09-19
• [Presentation] Susceptibility of isogeneic ginbuna Carassius langsdorfii to goldfish hematopoietic necorosis virus (CyHV-2) As a model species (2015)
  • Author(s): Nanjo A., T. Shibata, M. Saito, K. Yoshii, M. Tanaka, T. Nakanishi,T. Sakamoto, G. Kato, M. Sano
  • Organizer: 17th Int. Conf. on 'Diseases of Fish and Shellfish'
  • Place of Presentation: Canaria, スペイン
  • Year and Date: 2015-09-07
  • Int'l Joint Research
• [Presentation] IFNγ投与によるギンブナ好中球の活性化について (2015)
  • Author(s): 中嶋 城治、柴崎 康宏、松浦 雄太、難波 亜紀、間野 伸宏、中西 照幸
  • Organizer: 平成27年度日本比較免疫学会、第27回学術集会
  • Place of Presentation: 小浜市働く婦人の家（福井県小浜市）
  • Year and Date: 2015-08-21
• [Presentation] Edwardsiella tarda感染による脱シアリル化機構とその意義 (2015)
  • Author(s): Petros Chigwechokha、新吉沙弥香、本田晃伸、大木麗菜、荒木亨介、小松正治、宮城妙子、塩崎一弘
  • Organizer: 第34回日本糖質学会年会
  • Place of Presentation: 東京大学安田講堂（東京都文京区本郷）
  • Year and Date: 2015-07-31
• [Presentation] Monocolonal antibodies against CD4+ and CD8α+ of ginbuna crucian carp cross-react with zebrafish lymphocytes (2015)
  • Author(s): Miyazawa R., Y. Matsuura, Y. Shibasaki, T. Yabu, T. Nakanishi
  • Organizer: 国際比較免疫学会
  • Place of Presentation: Murcia スペイン
  • Year and Date: 2015-06-26
  • Int'l Joint Research
• [Presentation] Induction of T cell immunity by intestinal immunization with inactivated virus in ginbuna crucain carp (2015)
  • Author(s): Tajimi S, T. Somamoto, T. Nakanishi, M. Nakao
  • Organizer: 国際比較免疫学会
  • Place of Presentation: Murcia スペイン
  • Year and Date: 2015-06-26
  • Int'l Joint Research
• [Presentation] Exploring the serine protease involved in cell-mediated immunity in fish. (2015)
  • Author(s): Matsuura, Y., T. Yabu, H. Shiba, T. Moritomo, T. Nakanishi
  • Organizer: ASBMB 2015（米国生化学会・分子生物学会合同学会）
  • Place of Presentation: 米国、Boston
  • Year and Date: 2015-03-29
  • Invited
• [Presentation] コイ補体成分Properdinアイソフォームの機能解析 (2015)
  • Author(s): 吉岡和紀・鵜木陽子・杣本智軌・中尾実樹
  • Organizer: 平成27年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
  • Year and Date: 2015-03-28
• [Presentation] 穴あき病に対するニシキゴイ補体成C3の防御機構 (2015)
  • Author(s): 前田佑佳・杣本智軌・鶴田幸成・中尾実樹
  • Organizer: 平成27年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
  • Year and Date: 2015-03-28
• [Presentation] コイとギンブナにおけるコイヘルペスウイルスへの免疫応答の比較 (2015)
  • Author(s): 満崎敬子・中尾実樹・杣本智軌
  • Organizer: 平成27年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
  • Year and Date: 2015-03-28
• [Presentation] カンパチInterleukin-12の機能解析 (2015)
  • Author(s): 松本萌、荒木亨介、早志和真、丸吉浩太、劉倩、末武弘章、柳宗悦、今岡慶明、山本淳
  • Organizer: 平成27年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
  • Year and Date: 2015-03-28
• [Presentation] SMART理論による魚病細菌の選択培地の設計 (2015)
  • Author(s): 北野弘、荒木亨介、山本淳
  • Organizer: 日本魚病学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
  • Year and Date: 2015-03-08
• [Presentation] 魚種間における遺伝子発現比較のためのオーソロガス遺伝子の抽出 (2015)
  • Author(s): 高野倫一、近藤秀裕、安池元重、中村洋路、河東康彦、加藤豪司、坂井貴光、松山知正、栗田潤、藤原篤志、廣野育生、中易千早
  • Organizer: 日本魚病学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
  • Year and Date: 2015-03-08
• [Presentation] ヘルペスウイルス性造血器壊死症研究におけるクローンギンブナの有用性 (2015)
  • Author(s): 南條梓・柴田智也・齊藤真慧・吉井啓亮・田中深貴男・中西照幸・坂本崇・佐野元彦
  • Organizer: 日本魚病学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
  • Year and Date: 2015-03-08
• [Presentation] コイＢ細胞における自然免疫活性化能 (2015)
  • Author(s): 赤木良太, 長沢貴宏, 杣本智軌, 中尾実樹
  • Organizer: 日本水産学会九州支部会
  • Place of Presentation: 宮崎市民プラザ、宮崎市
  • Year and Date: 2015-01-10
• [Presentation] Effect of high-concentration ascorbic acid supplementation on disease resistance and some innate immune responses in rainbow trout (2014)
  • Author(s): Namba, A. T. Ishikawa, T. Yokozuka, T. Yabu, N. Mano, M. Sawada and T. Nakanishi
  • Organizer: 9th Symposium on Diseases on Asian Aquaculture (DAA9)
  • Place of Presentation: ベトナム、ホーチミン市
  • Year and Date: 2014-11-26
  • Invited
• [Presentation] Effect of adding goldfish kidney extract to cell culture medium on the growth of goldfish hematopoietic necrosis virus (2014)
  • Author(s): Shibata, T., A. Nanjyo, M. Saito, K. Yoshii, T. Ito, T. Nakanishi,T. Sakamoto and M. Sano
  • Organizer: 9th Symposium on Diseases on Asian Aquaculture (DAA9)
  • Place of Presentation: ベトナム、ホーチミン市
  • Year and Date: 2014-11-26
• [Presentation] Exploring the serine protease substrate involved in cell-mediated immunity in fish. (2014)
  • Author(s): Matsuura, Y., T. Yabu, H. Shiba, T. Moritomo, T. Nakanishi
  • Organizer: 9th Symposium on Diseases on Asian Aquaculture (DAA9)
  • Place of Presentation: ベトナム、ホーチミン市
  • Year and Date: 2014-11-26
  • Invited
• [Presentation] キンギョヘルペスウイルス性造血器壊死症ウイルスの増殖等の特性について (2014)
  • Author(s): 柴田智也・南條 梓・齊藤真慧・吉井啓亮・伊東尚史・中西照幸・坂本 崇・佐野元彦
  • Organizer: 日本魚病学会秋季大会
  • Place of Presentation: 九州大学、福岡市
  • Year and Date: 2014-09-23
• [Presentation] イシダイ、カワハギ、ヒラメおよびマダイに対する次世代シーケンサーを使用した免疫関連遺伝子の網羅的探索 (2014)
  • Author(s): 高野倫一、安池元重、中村洋路、加藤豪司、坂井貴光、松山知正、栗田潤、藤原篤志、近藤秀裕、廣野育生、中易千早
  • Organizer: 日本魚病学会秋季大会
  • Place of Presentation: 九州大学、福岡市
  • Year and Date: 2014-09-22
• [Presentation] Rag1欠損ゼブラフィッシュの免疫調節機構 (2014)
  • Author(s): 菅原亮太, 吉浦康寿, 杣本智軌, 中尾実樹
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 九州大学、福岡市
  • Year and Date: 2014-09-21
• [Presentation] カンパチInterleukin-12遺伝子の単離および発現解析 (2014)
  • Author(s): 松本萌、荒木亨介、丸吉浩太、山口愉生也、渡邊勇歩、早志和真、劉倩、柳宗悦、山本淳
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 九州大学、福岡市
  • Year and Date: 2014-09-21
• [Presentation] A new strategy to characterize different cell types from fish leukocytes using the bio-imaging technology of the confocal Raman microspectroscopy (2014)
  • Author(s): Aoki, T., M. Ando, H. Hamaguchi, J. Hikima, M. Sakai, T. Moritomo, T. Nakanishi and H. Takeyama
  • Organizer: Seventh International Symposium on Aquatic Animal Health(ISAAH-7)
  • Place of Presentation: 米国 ポートランド
  • Year and Date: 2014-08-31 – 2014-09-04
• [Presentation] 魚類のホルマリン不活化ワクチンによる液性免疫誘導と細胞性免疫抑制 (2014)
  • Author(s): 山﨑雅俊、荒木亨介、中西照幸、中易千早、松﨑吾朗、山本淳
  • Organizer: 第25回日本生体防御学会学術総会
  • Place of Presentation: 東北大学、仙台市
  • Year and Date: 2014-07-09
• [Presentation] Preparation of anti-Koi Herpes Virus IgY and its prophylactic effect. (2014)
  • Author(s): Hatta, H., Somamoto T, Atsushi. Satou, Kinjiro Morimoto
  • Organizer: EGG BANFF FORUM CONFERENCE
  • Place of Presentation: Poland Wroclaw
  • Year and Date: 2014-06-26
• [Presentation] Identification and characterization of novel Granzyme granzyme in fish (2014)
  • Author(s): Matsuura, Y., T. Yabu, Y. Shibasaki, H. Shiba, T. Moritomo, T. Nakanishi.
  • Organizer: ASBMB 2014（米国生化学会・分子生物学会合同学会）
  • Place of Presentation: 米国, サンディエゴ
  • Year and Date: 2014-04-29
  • Invited
• [Presentation] ギンブナのEdwardsiella tarda ホルマリン不活化ワクチンおよび弱毒生ワクチンにより誘導される二次免疫応答の比較
  • Author(s): 山崎雅俊・荒木亨介・増田鷹侑・中西照幸・中易千早・山本淳
  • Organizer: 平成26年度日本魚病学会春季大会
  • Place of Presentation: 函館国際ホテル
• [Presentation] コイインターロイキン2のT細胞増殖効果
  • Author(s): 森友忠昭・石村拡之・薮 健史・染谷和江・浅川雅清・中西照幸
  • Organizer: 平成26年度日本魚病学会春季大会
  • Place of Presentation: 函館国際ホテル
• [Presentation] 腸菌封入体を利用したヒラメ1型インターフェロンの調製
  • Author(s): 高野倫一、松山知正、坂井貴光、加藤豪司、中易千早
  • Organizer: 平成26年度日本魚病学会春季大会
  • Place of Presentation: 函館国際ホテル
• [Presentation] ギンブナIFNγrelのシグナル伝達機構
  • Author(s): 柴崎康宏、藪健史、松浦雄太、難波亜紀、間野伸宏、森友忠昭、中西照幸
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学水産学部
• [Presentation] ギンブナの同種移植片拒絶における4種類のIFNγアイソファームの役割
  • Author(s): 畑中ちひろ、柴崎康宏、松浦雄太、藪健史、森友忠昭、中西照幸
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学水産学部
• [Presentation] ギンブナのE. tarda感染に対するIFNγの投与効果
  • Author(s): 難波亜紀・柴崎康宏・松浦雄太・藪 健史・間野伸宏・中西照幸
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学水産学部
• [Presentation] 魚類新規グランザイムの同定
  • Author(s): 松浦雄太 薮健史 柴崎康宏 司馬肇 森友忠昭 中西照幸
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学水産学部
• [Presentation] アユB細胞で発現する遺伝子群の同定
  • Author(s): 加藤豪司、高野倫一、甲斐 渉、安池元重、中村洋路、藤原篤志、坂井貴光、松山知正、佐野菜採、中易千早
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学水産学部
• [Presentation] アユIgT遺伝子の構造解析および発現解析
  • Author(s): 加藤豪司、高野倫一、坂井貴光、松山知正、佐野菜採、川上 穣、中易千早
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学水産学部
• [Presentation] ギンブナのヘルパーＴ細胞および細胞障害性T細胞はEdwardsiella tarda感染に対する防御免疫において重要な役割を果たす
  • Author(s): 山崎雅俊、荒木亨介、中西照幸、中易千早、山本淳
  • Organizer: 平成２５年度日本水産増殖学会
  • Place of Presentation: 鹿児島大学
• [Presentation] ヒラメエドワジエラ症ワクチンにおける2種類の組換えインターフェロンのアジュバント効果
  • Author(s): 高野倫一、松山知正、坂井貴光、加藤豪司、中易千早
  • Organizer: 平成25年度日本魚病学会秋期大会
  • Place of Presentation: 三重大学
• [Presentation] ヒラメIL-6のサンドイッチELISAの確立
  • Author(s): 佐野菜採、松山知正、坂井貴光、高野倫一、加藤豪司、中易千早
  • Organizer: 平成25年度日本水産学会秋期大会
  • Place of Presentation: 三重大学
• [Presentation] 不活化ウイルスの経腸管感作によって誘導されるギンブナT細胞の免疫応答及び感染防御効果
  • Author(s): 多治見誠亮・杣本智軌・中西照幸・中尾実樹
  • Organizer: 平成25年度日本水産学会秋期大会
  • Place of Presentation: 三重大学
• [Presentation] ギンブナTh1様細胞のEdwardsiella tardaに対する感染防御における役割
  • Author(s): 山崎雅俊、荒木亨介、中西照幸、中易千早、山本淳
  • Organizer: 平成２５年度日本魚病学会秋季大会
  • Place of Presentation: 三重大学
• [Presentation] 魚類特有のインターフェロン、IFNγrelの機能解析
  • Author(s): 柴崎康宏，藪健史，有田希、岡本修太、畑中ちひろ、松浦雄太、山下倫明，森友忠昭，中西照幸
  • Organizer: 日本生化学会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] 同種移植鱗拒絶応答におけるギンブナグランザイムの発現動態解析
  • Author(s): 松浦雄太 薮健史 柴崎康宏 司馬肇 森友忠昭 中西照幸
  • Organizer: 日本生化学会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] ストレス誘導性アポトーシスはSAPK/JNKシグナルによる中性スフィンゴミエリナーゼ１のリン酸化が引金となる
  • Author(s): 藪健史，司馬肇，柴崎康宏，中西照幸，今村伸太朗，山下倫明
  • Organizer: 日本生化学会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] Comparative analysis of the adaptive immunity response to live and inactivated Edwarsiella tarda in gibuna crucian carp , Carassius auratus langsdorfii.
  • Author(s): Yamasaki, M., K. Araki, T. Nakanishi, C. Nakayasu, Y. Yoshiura, and A. Yamamoto
  • Organizer: 16th International Conference on Disease of Fish and Shellfish
  • Place of Presentation: Tampere, Finland
• [Presentation] Adaptive immune response in yellowtail Seriola quinqueradiata induced by formalin-killed Mycobacterium sp. in oil-adjuvant.
  • Author(s): Araki, K., Shimono, Y., Yamasaki, M., Matsumoto, S., Yanagi, S., Maeno, K., Yamamoto, A.
  • Organizer: 16th International Conference on Disease of Fish and Shellfish
  • Place of Presentation: Tampere, Finland
• [Presentation] Immunostimulation with recombinant interferon gamma enhances resistance of ginbuna crucian carp Carassius auratus langsdorfii to the intracellular parasitic bactrium Edwardsiella tarda.
  • Author(s): Namba, A., K. Kanai, Y. Shibasaki, T. Yabu, N. Mano and T. Nakanishi
  • Organizer: 16th International Conference on Disease of Fish and Shellfish
  • Place of Presentation: Tampere, Finland
• [Presentation] Serum interferon gamma （IFNγ） levels are affected by high-concentration ascorbic acid (ASA) supplementation in the rainbow trout Oncorhynchus mykiss.
  • Author(s): Kanai K. A. Namba, Y. Shibasaki, T. Yabu, H. Anzai, T. Ishikawa, T. Yokozuka, N. Mano and T. Nakanishi
  • Organizer: 16th International Conference on Disease of Fish and Shellfish
  • Place of Presentation: Tampere, Finland
• [Presentation] 魚類におけるインターフェロンγ産生細胞の同定
  • Author(s): 有田希、柴崎康宏、松浦雄太、藪健史、森友忠昭、中西照幸
  • Organizer: 日本比較免疫学会 第25回学術集会
  • Place of Presentation: 岡山理科大
• [Presentation] GVHD誘導に伴うドナーTリンパ球における免疫関連遺伝子の発現解析
  • Author(s): 岡本修太，柴崎康宏，松浦雄太，藪健史，森友忠昭，中西照幸
  • Organizer: 日本比較免疫学会 第25回学術集会
  • Place of Presentation: 岡山理科大
• [Presentation] コイの各臓器におけるγδT細胞の多様性について
  • Author(s): 宮前二朗、四反田聡，山口卓哉，森友忠昭，中西照幸
  • Organizer: 日本比較免疫学会 第25回学術集会
  • Place of Presentation: 岡山理科大
• [Presentation] コイIL-2関連サイトカインの遺伝子クローニングとT細胞培養系における発現解析
  • Author(s): 銘苅裕二，石原裕美，染谷和江，山口卓哉，藪健史，森友忠昭，中西照幸
  • Organizer: 日本比較免疫学会 第25回学術集会
  • Place of Presentation: 岡山理科大
• [Presentation] Characterizations of membrane-bound complement regulatory protein in Ginbuna Crucian Carp Carassius auratus langsdorfii
  • Author(s): Nur, I., Harada, H. Tsujikura, M., Somamoto, T., Nakao, M
  • Organizer: 日本比較免疫学会 第25回学術集会
  • Place of Presentation: 岡山理科大
• [Presentation] Requirement of contact for direct antibacterial activity of lymphocyte subpopulations in ginbuna crucian carp
  • Author(s): Tartor H, SK. Nayak, T Nakanishi
  • Organizer: First International Conference of Fish and Shellfish Immunology
  • Place of Presentation: Vigo, Spain
• [Presentation] N-acetyl-D-glucosamine-binding immunoglobulin M of fugu (Takifugu rubripes) inhibits the growth of fish pathogenic bacteria: A novel function of teleost antibody.
  • Author(s): Tsuitsui, S., Ariji, T, Sato, A., Yoshida, T., Yamamura, N., Odaka, T., Araki, K., Suetake, H., Miyadai, T., Nakamura, O.
  • Organizer: First International Conference of Fish and Shellfish Immunology
  • Place of Presentation: Vigo, Spain
• [Book] Vaccine Design: Methods and Protocols (2016)
  • Author(s): Nayak S K and Nakanishi T.
  • Total Pages: 854
  • Publisher: Springer
• [Book] The evolution of complement system functions and pathways in vertebrates. (2016)
  • Author(s): Nakao M, Somamoto T.
  • Total Pages: 500
  • Publisher: Elsevier
• [Book] EOLSS (Encyclopedia of Life Support Systems) (2015)
  • Author(s): Nakanishi, T.
  • Total Pages: 600
  • Publisher: Eolss Publishers Co Ltd