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Establishment of the freshness assessment system for fresh produces based on the comprehensive analysis of the cell membrane lipid peroxidation and decomposition

Research Project

Project/Area Number 25292155
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Agricultural environmental engineering/Agricultural information engineering
Research InstitutionGifu University

Principal Investigator

Nakano Kohei  岐阜大学, (連合)農学研究科(研究院), 教授 (20303513)

Co-Investigator(Kenkyū-buntansha) KUROKI Shinichiro  神戸大学, 農学研究科, 助教 (00420505)
YOSHIDA Makoto  神奈川県農業技術センター, 生産環境部, 課長 (20503650)
SHIINA Takeo  千葉大学, 園芸学研究科, 教授 (40353974)
NAKAMURA Nobutaka  国立研究開発法人農業・食品産業機構, 食品総合研究所, 主任研究員 (50353975)
SOGA Ayaka  神奈川県農業技術センター, 生産環境部, 主任研究員 (60503638)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Keywords鮮度評価 / 脂質過酸化 / 青果物 / 遺伝子発現 / 揮発性成分 / 水透過係数 / 農業工学 / ポストハーベスト工学 / 水伝導係数 / 揮発性化合物
Outline of Final Research Achievements

The changes of the senescence-relating genes expression level, the qualitative and quantitative characteristics of lipids, the volatile compounds, and the physicochemical property of cell membrane of fruits and vegetables were discussed to establish the novel freshness assessment theory based on the lipid peroxidation and decomposition.
The freshness-relating gene and volatile compound clusters could be identified. The degradation rate of saturated fatty acids was almost same as unsaturated ones, and lipid peroxidation rate differed depending on the part of the leaf. Moreover, it was suggested that the progress of the cell membrane hypofunction occurred by the accumulation of lipid peroxides and following phase transition. These findings will give important knowledge for further research on freshness assessment of fruits and vegetables.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (35 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (27 results) (of which Int'l Joint Research: 1 results,  Invited: 9 results)

  • [Int'l Joint Research] アンダラス大学(インドネシア)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Effective transport and storage condition for preserving the quality of ‘Jiro’ persimmon in export market2016

    • Author(s)
      Fahmy, K., Nakano, K.
    • Journal Title

      Agriculture and Agricultural Science Procedia

      Volume: 9 Pages: 279-290

    • DOI

      10.1016/j.aaspro.2016.02.115

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Response mechanisms of cabbage head to different strength levels ofmechanical impact stress2015

    • Author(s)
      Shiina, T., Umehara, H., Yoshida, M., Soga. A., Kaneta. T., Nakamura, N., Ito. Y., Nakano, K., Thammawong, M.
    • Journal Title

      Acta Horticulturae

      Volume: 1091 Pages: 311-318

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oscillations of Respiration-relating Gene Expression in Postharvest Cabbage Head under Different Controlled Atmosphere (CA) Storage Regimes2015

    • Author(s)
      Thammawong, M., Yoshida, M., Umehara, H., Kaneta, T., Soga, A., Nakamura, N., Ito, Y., Nakano, K., Shiina, T.
    • Journal Title

      Acta Horticulturae

      Volume: 1091 Pages: 303-310

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Investigation on quantitative index of chilling injury in cucumber fruit based on the electrolyte leakage and malondialdehyde content2015

    • Author(s)
      Fahmy, K., Nakano, K., Fidela, V.
    • Journal Title

      International Journal on Advanced Science Engineering Information Technology

      Volume: 5(3) Issue: 3 Pages: 222-225

    • DOI

      10.18517/ijaseit.5.3.532

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 農産物・食品の安全と品質の確保技術(第10回)-生鮮野菜の定量的な鮮度評価技術について-2015

    • Author(s)
      中野浩平
    • Journal Title

      農業食料工学会誌

      Volume: 77(3) Pages: 154-158

    • NAID

      40020473629

    • Related Report
      2015 Annual Research Report
    • Open Access
  • [Journal Article] 保蔵温度の異なる緑熟トマトの追熟における果皮色,積算エチレン生成量および果実品質の変化2014

    • Author(s)
      菅理哉, 小出章二, 折笠貴寛, 中村宣貴, 椎名武夫
    • Journal Title

      日本食品保蔵科学会誌

      Volume: 40(6) Pages: 279-283

    • NAID

      40020306699

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nondestructive freshness evaluation of spinach leaves under low oxygen storage using visible and near infrared spectroscopy2014

    • Author(s)
      Kuroki, S., Mizutani, R., Tachikawa, Y., Nakano, K.
    • Journal Title

      2013 IFAC Bio-Robotics Conference

      Volume: 1(1) Issue: 4 Pages: 302-306

    • DOI

      10.3182/20130327-3-jp-3017.00069

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 脂質過酸化・分解現象に着目した青果物の鮮度評価法2015

    • Author(s)
      中野浩平,Manasikan THAMMAWONG,黒木信一郎,中村宣貴,曽我綾香,吉田誠,椎名武夫
    • Organizer
      農業情報学会2015年度秋期大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-12-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] ホウレンソウ葉細胞膜の物理化学的特性と鮮度との関係2015

    • Author(s)
      小泉高陽,黒木信一郎,曽我綾香,吉田誠,中村宣貴,椎名武夫,中野浩平
    • Organizer
      農業環境工学関連5学会2015合同大会
    • Place of Presentation
      岩手
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 青果物のTET品質管理とそのツールとしての遺伝子発現解析2015

    • Author(s)
      椎名武夫,Manasikan THANMAWONG,中村宣貴,吉田誠,曽我綾香,黒木信一郎,中野浩平
    • Organizer
      農業環境工学関連5学会2015合同大会
    • Place of Presentation
      岩手
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] ブロッコリーにおける物理ストレス指標遺伝子の探索2015

    • Author(s)
      Manasikan THANMAWONG,梅原仁美,中村宣貴,曽我綾香,吉田誠,黒木信一郎,中野浩平,椎名武夫
    • Organizer
      農業環境工学関連5学会2015合同大会
    • Place of Presentation
      岩手
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 農産物の鮮度指標となる揮発性成分の検索2015

    • Author(s)
      吉田誠,曽我綾香,黒木信一郎,中村宣貴,椎名武夫,Manasikan THAMMAWONG,中野浩平
    • Organizer
      農業環境工学関連5学会2015合同大会
    • Place of Presentation
      岩手
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 施肥条件が農産物収穫後の品質保持に及ぼす影響2015

    • Author(s)
      曽我綾香,吉田誠,黒木信一郎,中村宣貴,椎名武夫,Manasikan THAMMAWONG,中野浩平
    • Organizer
      農業環境工学関連5学会2015合同大会
    • Place of Presentation
      岩手
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 過酸化脂質を指標とした青果物の鮮度評価2015

    • Author(s)
      髙木絢子,Manasikan THAMMAWONG,黒木信一郎,中村宣貴,曽我綾香,吉田誠,椎名武夫,中野浩平
    • Organizer
      農業環境工学関連5学会2015合同大会
    • Place of Presentation
      岩手
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] Current Status and Issues of the Quality Management System for Fresh Produes in Japan for Sustainable Agriculture2015

    • Author(s)
      K. Nakano
    • Organizer
      Int. Conf. on Food, Agriculture and Natural Resouce 2015 (FANRes2015)
    • Place of Presentation
      Jember, Indonesia
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] キャベツの老化過程における脂質過酸化物量の変化2015

    • Author(s)
      淺野良太,中野浩平
    • Organizer
      農業食料工学会関西支部第133回例会
    • Place of Presentation
      京都
    • Year and Date
      2015-03-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] 過酸化脂質の定量による青果物のストレス評価 ー測定系の確立とUV処理試験2015

    • Author(s)
      高木絢子,中野浩平
    • Organizer
      農業食料工学会関西支部第133回例会
    • Place of Presentation
      京都
    • Year and Date
      2015-03-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] 農産物品質関連遺伝子の解析と品質評価2014

    • Author(s)
      椎名武夫
    • Organizer
      平成26年度研究人材活性化対策事業研究推進支援研修
    • Place of Presentation
      神奈川
    • Year and Date
      2014-12-04 – 2014-12-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 食品の流通(物流)・保存技術の最前線2014

    • Author(s)
      椎名武夫
    • Organizer
      日本化学会 第4回CSJ化学フェスタ2014
    • Place of Presentation
      東京
    • Year and Date
      2014-10-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Measurement and evaluation of dysfunction of cell membranes in fruits and vegetables during low oxygen storage2014

    • Author(s)
      Kuroki, S., Tachikawa, Y., Koizumi, K., Soga, A., Yoshida, M., Nakamura, N., Shiina, T., Nakano, K
    • Organizer
      International Conference on Sustainable Agriculture, Food and Energy (SAFE2014)
    • Place of Presentation
      Dempasar, Indonesia
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Easy and Low-cost Evaluation Method for the Freshness of Green Beans Based on the Stachyose Detection2014

    • Author(s)
      Nakano,K
    • Organizer
      International Conference of Sustaintable Agriculture, Food and Energy
    • Place of Presentation
      Dempasar, Indonesia
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Sustainablity Framework for Post-harvest Techonology in Japan2014

    • Author(s)
      Nakano, K.
    • Organizer
      30th cellebration of Warmadawa University
    • Place of Presentation
      Dempasar, Indonesia
    • Year and Date
      2014-09-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 低酸素貯蔵がホウレンソウ葉の細胞膜機能劣化に与える影響2014

    • Author(s)
      立川雄樹, 黒木信一郎, 小泉高陽, 曽我綾香, 吉田誠, 中村宣貴, 椎名武夫, 中野浩平
    • Organizer
      日本生物環境工学会2014年東京大会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-08 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] コマツナの老化過程におけるカルボニル化合物含量の変化2014

    • Author(s)
      吉野哲史,黒木信一郎,曽我綾香,吉田誠,中村宣貴,椎名武夫,中野浩平
    • Organizer
      2014年度農業施設学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2014-08-27 – 2014-08-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 農産物の鮮度指標となる揮発性成分の検索2014

    • Author(s)
      吉田誠,曽我綾香,中村宣貴,椎名武夫,黒木信一郎,中野浩平
    • Organizer
      第63回日本食品保蔵科学会大会
    • Place of Presentation
      長野
    • Year and Date
      2014-06-28 – 2014-06-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 施肥条件が農産物収穫後の品質保持に及ぼす影響2014

    • Author(s)
      曽我綾香,吉田誠,中村 宣貴,椎名 武夫,黒木信一郎,中野浩平
    • Organizer
      第63回日本食品保蔵科学会大会
    • Place of Presentation
      長野
    • Year and Date
      2014-06-28 – 2014-06-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 青果物のコールドチェーンと品質保持技術2014

    • Author(s)
      椎名武夫
    • Organizer
      農業食料工学会2014年度シンポジウム -フードテクノロジー(フーテック)フォーラム
    • Place of Presentation
      東京
    • Year and Date
      2014-06-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 細胞膜の物理化学的特性を指標とした青果物の品質評価法の開発2014

    • Author(s)
      黒木信一郎, 小泉高陽, 立川雄樹, 曽我綾香, 吉田誠, 中村宣貴, 椎名武夫, 中野浩平
    • Organizer
      第73回農業食料工学会
    • Place of Presentation
      沖縄
    • Year and Date
      2014-05-16 – 2014-05-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細胞膜脂質の過酸化分解系の網羅解析に基づく鮮度アセスメントシステムの確立 -わさび菜の貯蔵中における脂肪酸組成の変化-2014

    • Author(s)
      中野浩平,黒木信一郎,曽我綾香,吉田誠,中村宣貴,椎名武夫
    • Organizer
      第73回農業食料工学会
    • Place of Presentation
      沖縄
    • Year and Date
      2014-05-16 – 2014-05-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低温障害による生体膜機能劣化とその非破壊計測技術開発2013

    • Author(s)
      黒木信一郎
    • Organizer
      農林水産消費安全技術センター(FAMIC)神戸センター/食の安全・安心科学センター合同発表会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ホウレンソウ葉内脂質過酸化レベルの非破壊モニタリング技術の開発2013

    • Author(s)
      立川雄樹, 黒木信一郎, 中野浩平
    • Organizer
      第72回農業食料工学会年次大会
    • Place of Presentation
      帯広
    • Related Report
      2013 Annual Research Report
  • [Presentation] キュウリ果実の低温障害進行過程における生体膜機能劣化の非破壊計測2013

    • Author(s)
      小湊貴子, 黒木信一郎, 中野浩平
    • Organizer
      第72回農業食料工学会年次大会
    • Place of Presentation
      帯広
    • Related Report
      2013 Annual Research Report
  • [Presentation] A new quantitative evaluation method for freshness of vegetables for reducing postharvest losses2013

    • Author(s)
      Nakano, K.
    • Organizer
      International Conference Sustainable Agriculture, Food, and Energy 2013 (SAFE2013)
    • Place of Presentation
      Padang, Indonesia
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Freshness assessment of fresh produces for preventing postharvest losses2013

    • Author(s)
      Nakano, K.
    • Organizer
      ASEAN Regional Conference on Food Security 2013 (ARCoFS2013)
    • Place of Presentation
      Penag, Malaysia
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2013-05-21   Modified: 2019-07-29  

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