| Project/Area Number |
25292160
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | Kobe University |
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Principal Investigator |
MIYAKE MASASHI 神戸大学, 統合研究拠点, 名誉教授 (60093316)
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| Co-Investigator(Kenkyū-buntansha) |
HARAYAMA Hiroshi 神戸大学, 大学院農学研究科, 教授 (30281140)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | 着床前発生 / タイトジャンクション / claudins / グルコース代謝 / 活性化2倍体 / ブタ / 胚盤胞形成 / クローディン / 応用動物科学 / 着床前発生能力 / 単為発生2倍体 |
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| Outline of Final Research Achievements |
The expression of claudin family, tight junction (TJ) were examined in pig blastocysts derived from activated diploids. Blastocysts expressed both mRNAs and proteins of claudin-1, -2, -4, -6, -7, -8, -9, -12 and -14. While claudin-4, -6 and -7 localized along the cell-boundary TJ structure, the other claudins were cytoplasmic. These results suggest that claudin-4, -6 and -7 are important for the TJ functions in pig blastocysts. Knockdown of claudin-1 and -4 by their siRNA severely suppressed the early cleavage.
To examine whether expression patterns of the claudins will be employed as markers for the potential developmental ability of in vitro produced blastocysts in future, features of glucose metabolism was examined in pig preimplantation embryos. It was shown that recommendation of carbohydrates are different depending on the preimplantation developmental stages and hexose biosynthesis pathway and GlcNAcylation have important roles in pig preimplantation development.
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