Elucidation of the growth mechanism of bovine small piroplasma

• Project/Area Number: 25292169
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: Yokoyama Naoaki 帯広畜産大学, 原虫病研究センター, 教授 (80301802)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 小型ピロプラズマ / シゾント / ピロプラズム / スポロゾイト / 牛 / ピロプラズマ病 / 増殖 / 赤血球 / マダニ / メロゾイト / 貧血 / 小型ピロプラズマ病 / 増殖メカニズム / 遺伝子多型 / ウシ / MPSP / SCIDマウス / マイコプラズマ / ゲノム

Outline of Final Research Achievements

We prepared an antibody that recognizes the schizont stage of small piroplasm to observe the schizont-infected cells, but such cells were not detected. However, we were able to 1) Sequence the whole genome of two genotypes small piroplasm, 2) Establish a “tick-SCID mouse” model for experimental infection and thereby analyze the gene expression profiles of sporozoite stage, 3) Identify the RBC receptors targeted by protozoan membrane proteins (p23 and MPSP), and 4) Investigate the epidemiology and genetic diversity of small piroplasm in Japan and several endemic countries. Resultantly, we have gained a more thorough understanding of the mechanisms involving the growth of bovine small piroplasm. Furthermore, we have comprehensively reviewed the ecology, epidemiology, genetic polymorphism, and evolution of the small piroplasm in an article that was published in an international scientific journal.

Research Products

• [Int'l Joint Research] フエ大学/フエ農林大学(ベトナム)
• [Int'l Joint Research] 国立獣医学研究所(スリランカ)
• [Int'l Joint Research] 獣医学研究所(スリランカ)
• [Int'l Joint Research] モンゴル農業大学(モンゴル)
• [Int'l Joint Research] サダト大学(エジプト)
• [Journal Article] 牛小型ピロプラズマ病対策を目的としたマダニ対策プログラムが北海道共同牧野の放牧育成牛の繁殖成績に与えた影響 (2016)
  • Author(s): 前野和利、松井伸一、河合孝弘、鈴木真生、千葉友行、横山直明、猪熊 壽
  • Journal Title: 産業動物臨床医誌 Volume: 7 Pages: 169-174
  • Peer Reviewed
• [Journal Article] Babesia ovata: Taxonomy, phylogeny and epidemiology (Review). (2016)
  • Author(s): Sivakumar, T., Igarashi, I., and Yokoyama, N.
  • Journal Title: Vet. Parasitol. Volume: 229 Pages: 99-106
  • DOI: 10.1016/j.vetpar.2016.10.006
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Epidemiology of bovine hemoprotozoa parasites in cattle and water buffalo in Vietnam (2016)
  • Author(s): Weerasooriya, G., Sivakumar, T., Lan, D.T., Long, P.T., Takemae, H., Igarashi, I., Inoue, N., and Yokoyama, N.
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 8 Pages: 1361-1367
  • DOI: 10.1292/jvms.16-0099
  • NAID: 130005261519
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A longitudinal study of Babesia and Theileria infections in cattle in Sri Lanka. (2016)
  • Author(s): Sivakumar, T., Kothalawala, H., Weerasooriya, G., Silva, S.S.P., Puvanendiran, S., Munkhjargal, T., Igarashi, I., and Yokoyama, N.
  • Journal Title: Veterinary Parasitology: Regional Studies and Reports Volume: 6 Pages: 20-27
  • DOI: 10.1016/j.vprsr.2016.11.009
  • NAID: 120006390921
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 北海道・釧路管内の２共同牧野における放牧牛及び生息マダニのTheileria oriantalis感染状況とマダニ対策の効果 (2015)
  • Author(s): 前野和利、松井伸一、河合孝弘、吉川真生、横山直明、猪熊壽
  • Journal Title: 日獣会誌 Volume: 68 Pages: 231-238
  • Peer Reviewed
• [Journal Article] 馬ピロプラズマ病 (2015)
  • Author(s): 五十嵐郁男、横山直明
  • Journal Title: Jpn J Vet Parasitol. Volume: 14 Pages: 83-92
  • Peer Reviewed
• [Journal Article] The PCR detection and phylogenetic characterization of Babesia microti in questing ticks in Mongolia. (2015)
  • Author(s): Tuvshintulga B, Sivakumar T, Battsetseg B, Narantsatsaral SO, Enkhtaivan B, Battur B, Hayashida K, Okubo K, Ishizaki T, Inoue N, Igarashi I, Yokoyama N.
  • Journal Title: Parasitol Int. Volume: 64 Issue: 6 Pages: 527-532
  • DOI: 10.1016/j.parint.2015.07.007
  • NAID: 120006390873
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Optimization of a Fluorescence-Based Assay for Large-Scale Drug Screening against Babesia and Theileria Parasites. (2015)
  • Author(s): Rizk, M.A., El-Sayed, S.A., Terkawi, M.A., Youssef, M.A., El Said el Sel, S., Elsayed, G., El-Khodery, S., El-Ashker, M., Elsify, A., Omar, M., Salama, A., Yokoyama, N., and Igarashi, I.
  • Journal Title: PLoS One. Volume: 10 Issue: 4 Pages: e0125276-e0125276
  • DOI: 10.1371/journal.pone.0125276
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An epidemiological survey of bovine Babesia and Theileria parasites in cattle, buffaloes, and sheep in Egypt. (2015)
  • Author(s): Elsify, A., Sivakumar, T., Nayel, M., Salama, A., Elkhtam, A., Rizk, M., Mosaab, O., Sultan, K., Elsayed, S., Igarashi, I., and Yokoyama, N.
  • Journal Title: Parasitol. Int. Volume: 64 Issue: 1 Pages: 79-85
  • DOI: 10.1016/j.parint.2014.10.002
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Macrophages are the determinant of resistance to and outcome of nonlethal Babesia microti infection in mice. (2015)
  • Author(s): Terkawi MA, Cao S, Herbas MS, Nishimura M, Li Y, Moumouni PF, Pyarokhil AH, Kondoh D, Kitamura N, Nishikawa Y, Kato K, Yokoyama N, Zhou J, Suzuki H, Igarashi I, Xuan X.
  • Journal Title: Infect Immun. Volume: 83 Issue: 1 Pages: 8-16
  • DOI: 10.1128/iai.02128-14
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evaluation of inhibitory effect of methylene blue against Babesia and Theileria parasites. (2015)
  • Author(s): Tuvshintulga, B., Sivakumar, T., Salama, A.A., Yokoyama, N., and Igarashi, I.
  • Journal Title: J. Protozool. Res. Volume: 25 Pages: 18-28
  • NAID: 120006391034
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] First detection of Babesia venatorum (EU1) in Ixodes persulcatus ticks in Mongolia. (2015)
  • Author(s): Tuvshintulga, B., Battsetseg, B., Battur, B., Myagmarsuren, P., Narantsatsral, S., Sivakumar, T., Takemae, H., Igarashi, I., Inoue, N., and Yokoyama, N.
  • Journal Title: J. Protozool. Res. Volume: 25 Pages: 29-37
  • NAID: 120006391035
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evolution and genetic diversity of Theileria. (2014)
  • Author(s): Sivakumar T, Hayashida K, Sugimoto C, Yokoyama N.
  • Journal Title: Infect. Genet. Evol. Volume: 27 Pages: 250-263
  • DOI: 10.1016/j.meegid.2014.07.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genetic characterization of Babesia and Theileria parasites in water buffaloes in Sri Lanka. (2014)
  • Author(s): Sivakumar T, Tattiyapong M, Fukushi S, Hayashida K, Kothalawala H, Silva SS, Vimalakumar SC, Kanagaratnam R, Meewewa AS, Suthaharan K, Puvirajan T, de Silva WK, Igarashi I, Yokoyama N.
  • Journal Title: Vet. Parasitol. Volume: 200 Issue: 1-2 Pages: 24-30
  • DOI: 10.1016/j.vetpar.2013.11.029
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 小型ピロプラズマの生態と疫学 (2014)
  • Author(s): 横山直明、林田京子
  • Journal Title: 臨床獣医 Volume: 32 Pages: 21-24
• [Journal Article] Interaction between Theileria orientalis 23-kDa piroplasm membrane protein and heparin. (2014)
  • Author(s): Takemae H, Sugi T, Kobayashi K, Murakoshi F, Recuenco FC, Ishiwa A, Inomata A, Horimoto T, Yokoyama N, Kato K.
  • Journal Title: Jpn. J. Vet. Res. Volume: 62 Pages: 17-24
  • NAID: 120005438296
  • Peer Reviewed
• [Journal Article] Analyses of the binding between Theileria orientalis major piroplasm surface proteins and bovine red blood cells. (2014)
  • Author(s): Takemae H, Horimoto T, et al.
  • Journal Title: Vet. Rec. Volume: 175 Issue: 6 Pages: 149-149
  • DOI: 10.1136/vr.102535
  • Peer Reviewed
• [Journal Article] Genetic diversity within Theileria orientalis parasites detected in Sri Lankan cattle. (2013)
  • Author(s): Sivakumar, T., Yoshinari, T., Igarashi, I., Kothalawala, H., Abeyratne, S. A. E., Vimalakumar, S. C., Meewewa, A. S., Kuleswarakumar, K., Chandrasiri, A. D. N., and Yokoyama, N.
  • Journal Title: Ticks Tick Borne Dis. Volume: 4 Issue: 3 Pages: 235-241
  • DOI: 10.1016/j.ttbdis.2012.11.009
  • Peer Reviewed
• [Journal Article] Interference between Theileria orientalis and hemotropic Mycoplasma spp). (hemoplasmas) in grazine cattle. (2013)
  • Author(s): Tagawa M. Ybañez AP. Matsumoto K. Yokoyama N. Inokuma H.
  • Journal Title: Veterinary Parasitology Volume: 195(1-2) Issue: 1-2 Pages: 165-168
  • DOI: 10.1016/j.vetpar.2012.12.041
  • Peer Reviewed
• [Journal Article] Molecular survey of bovine vector-borne pathogens in Cebu (2013)
  • Author(s): Ybanez, A.P., Sivakumar, T., Ybanez, R.H., Vincoy, M.R., Tingson, J.A., Perez, Z.O., Gabotero, S.R., Buchorno, L.P., Inoue, N., Matsumoto, K., Inokuma, H., and Yokoyama, N
  • Journal Title: Philippines. Vet. Parasitol Volume: 196 Issue: 1-2 Pages: 13-20
  • DOI: 10.1016/j.vetpar.2013.02.013
  • Peer Reviewed
• [Journal Article] PCR Detection and Genetic Diversity of Bovine Hemoprotozoan Parasites in Vietnam (2013)
  • Author(s): Sivakumar, T., Lan, D.T., Long, P.T., Yoshinari, T., Tattiyapong, M., Guswanto, A., Okubo, K., Igarashi, I., Inoue, N., Xuan, X., and Yokoyama, N
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 11 Pages: 1455-1462
  • DOI: 10.1292/jvms.13-0221
  • NAID: 130003362251
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Presentation] スリランカ国の牛におけるバベシア及びタイレリアの分子疫学調査 (2016)
  • Author(s): Sivakumar Thillaiampalam、五十嵐郁男、横山直明
  • Organizer: 第57回日本熱帯医学会大会
  • Place of Presentation: 一橋大学（東京都千代田区）
  • Year and Date: 2016-11-05
• [Presentation] Molecular epidemiology of Babesia and Theileria, and Trypanosoma in cattle and water buffaloes in Sri Lanka. (2015)
  • Author(s): Sivakumar T, Kothalawala H, Silva SSP, Yokoyama N.
  • Organizer: 57th Annual Convention of the Sri Lanka Veterinary Association
  • Place of Presentation: Oak-Ray and Earl's Regency (Kandy, Sri Lanka)
  • Year and Date: 2015-07-19
  • Int'l Joint Research
• [Presentation] The molecular diagnostic tools for bovine piroplasmosis (2015)
  • Author(s): Naoaki Yokoyama
  • Organizer: 57th Annual Convention of the Sri Lanka Veterinary Association
  • Place of Presentation: Oak-Ray and Earl's Regency (Kandy, Sri Lanka)
  • Year and Date: 2015-07-19
  • Int'l Joint Research / Invited
• [Presentation] Genetic diversity of Theileria (2014)
  • Author(s): 横山直明、Sivakumar Thillaiamapalam, 林田京子、杉本千尋
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] タイレリア原虫の比較ゲノム解析と病原性進化に関する研究 (2014)
  • Author(s): 林田京子
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌）
  • Year and Date: 2014-09-09 – 2014-09-12
  • Invited
• [Presentation] Theileria orientalis原虫2株の全ゲノム解読とタイレリアの病原性に関わる遺伝的多様性の解明 (2014)
  • Author(s): 林田京子、原 雄一郎、阿部貴志、服部正平、横山直明、杉本千尋
  • Organizer: 第83回日本寄生虫学会
  • Place of Presentation: 愛媛大学
• [Remarks] 帯広畜産大学・原虫病研究センター ホームページ
  • URL: http://www.obihiro.ac.jp/~protozoa/index.html