Developments for new tick prevantion tageting Toll signal pathway

• Project/Area Number: 25292173
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Kagoshima University
• Principal Investigator: TANAKA TETSUYA 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (00322842)
• Co-Investigator(Kenkyū-buntansha): 松尾 智英 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (50383667) ; 辻 尚利 北里大学, 医学部, 教授 (70355171)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: マダニ / Toll様関連因子(TLR) / TRAF関連因子(TRAFD1) / ディフェンシン / Toll 様関連因子(TLR) / フェリチン / ペルオキシレドキシン / グルタチオンSトランスフェラーゼ / リポソーム / TRAFD1 / TRAF経路

Outline of Final Research Achievements

Toll like receptor (TLR) and tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) are very important to protect againt pathogen infection. Therefore, we alalyzed to idetify and characterize TLR and TRAFD1 from the hard tick Haemaphysalis longicornis. Knockdown of TRAFD1 gene by RNA interference did not affect blood feeding or oviposition. However, TRAFD1 silencing affected the expression of the longicin gene, a defensin-like molecule. On the other hand,recombinant TLR demonstrated growth inhibition activity against B. gibsoni in vitro without a hemolytic effect at used concentration .These results suggested that TLR and TRAFD1 strongly affected innate immunity of ticks.

Research Products

• [Journal Article] 2-Cys peroxiredoxin is required in successful blood-feeding, reproduction, and antioxidant response in the hard tick Haemaphysalis longicornis (2016)
  • Author(s): Kusakisako K, Galay RL, Umemiya-Shirafuji R, Hernandez EP, Maeda H, Talactac MR, Tsuji N, Mochizuki M, Fujisaki K, Tanaka T.
  • Journal Title: Parasites and Vectors Volume: 9 Issue: 1 Pages: 457-457
  • DOI: 10.1186/s13071-016-1748-2
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice (2016)
  • Author(s): Yuina Ono, Makoto Matsubayashi, Hiroaki Kawaguchi, Masashi Tsujio, Masanobu Mizuno, Tetsuya Tanaka, Tatsunori Masatani, Toshihiro Matsui, Tomohide Matsuo
  • Journal Title: Acta Parasitologica Volume: 115(1) Issue: 1 Pages: 211-5
  • DOI: 10.1007/s00436-015-4737-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Role of the tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) from the hard tick Haemaphysalis longicornis in immunity against bacterial infection. (2016)
  • Author(s): Takechi R, Galay RL, Matsuo T, Maeda H, Kusakisako K, Talactac MR, Mochizuki M, Fujisaki K, Tanaka T.
  • Journal Title: Ticks and Tick-borne diseases Volume: 7 Issue: 1 Pages: 36-45
  • DOI: 10.1016/j.ttbdis.2015.08.002
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A novel C-type lectin with triple carbohydrate recognition domains has critical roles for the hard tick Haemaphysalis longicornis against Gram-negative bacteria. (2016)
  • Author(s): Maeda H, Miyata T, Kusakisako K, Galay RL, Talactac MR, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T.
  • Journal Title: Developmental & Comparative Immunology Volume: 57 Pages: 38-47
  • DOI: 10.1016/j.dci.2015.12.015
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Impaired cellular immune response to injected bacteria after knockdown of ferritin genes in the hard tick Haemaphysalis longicornis. (2016)
  • Author(s): Galay RL, Takechi R, Umemiya-Shirafuji R, Talactac MR, Maeda H, Kusakisako K, Mochizuki M, Fujisaki K, Tanaka T.
  • Journal Title: Parasitology International Volume: 65 Issue: 3 Pages: 251-257
  • DOI: 10.1016/j.parint.2016.01.007
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus. (2016)
  • Author(s): Talactac MR, Yoshii K, Maeda H, Kusakisako K, Hernandez EP, Tsuji N, Fujisaki K, Galay RL, Tanaka T, Mochizuki M.
  • Journal Title: Parasit Vectors Volume: 9 Issue: 1 Pages: 59-59
  • DOI: 10.1186/s13071-016-1344-5
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Dynamics of Theileria orientalis genotype population in cattle in a year-round grazing system. (2016)
  • Author(s): Masatani, T., Yoshihara, S., Matsubara, A., Gotoh, T., Takahashi, H., Tanaka, T., Andoh, M., Endo, Y. and Matsuo, T.
  • Journal Title: Acta Parasitol. Volume: 61 (2) Issue: 2 Pages: 419-424
  • DOI: 10.1515/ap-2016-0056
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Improvement of the cryopreservation method for the Babesia gibsoni parasite by using commercial freezing media (2016)
  • Author(s): Kusakisako, K., Masatani, T., Yada Y., Talactac, M. R., Hernandez, E. P., Maeda, H., Mochizuki, M. and Tanaka, T.
  • Journal Title: Parasitol. Int. Volume: In press Issue: 5 Pages: 532-535
  • DOI: 10.1016/j.parint.2016.02.012
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Induction of gene silencing in Haemaphysalis longicornis ticks through immersion in double-stranded RNA (2016)
  • Author(s): Galay, R. L., Hernandez, E. P., Talactac, M. R., Maeda, H., Kusakisako, K., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
  • Journal Title: Ticks Tick Borne Dis. Volume: In press Issue: 5 Pages: 813-816
  • DOI: 10.1016/j.ttbdis.2016.03.018
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Functional analysis of recombinant 2-Cys peroxiredoxin from the hard tick Haemaphysalis longicornis (2015)
  • Author(s): Kusakisako K, Masatani T, Miyata T, Galay RL, Maeda H, Talactac MR, Tsuji N, Mochizuki M, Fujisaki K, Tanaka T.
  • Journal Title: Insect Molecular Biology Volume: 25 Issue: 1 Pages: 16-23
  • DOI: 10.1111/imb.12193
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evaluation of Eimeria krijgsmanni as a murine model for testing the efficacy of anti-parasitic agents (2015)
  • Author(s): Takeo, T., Tanaka, T., Matsubayashi, M., Tsujio, M., Umemiya-Shirafuji, R., Tsuji N., Fujisaki K., Matsui, T. and Matsuo, T.
  • Journal Title: Acta Parasitol. Volume: 60 (2) Pages: 190-195
  • Peer Reviewed
• [Journal Article] Identification of the Babesia-responsive leucine-rich repeat domain-containing protein from the hard tick Haemaphysalis longicornis. (2015)
  • Author(s): Maeda H, Kurisu K, Miyata T, Kusakisako K, Galay RL, Rio TM, Mochizuki M, Fujisaki K, Tanaka T.
  • Journal Title: Parasitol. Res. Volume: 114 Issue: 5 Pages: 1793-1802
  • DOI: 10.1007/s00436-015-4365-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The widely distributed hard tick, <i>Haemaphysalis longicornis</i>, can retain canine parvovirus, but not be infected in laboratory condition (2015)
  • Author(s): Mori H., Tanaka, T. and Mochizuki, M.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 4 Pages: 405-411
  • DOI: 10.1292/jvms.14-0199
  • NAID: 130005067022
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] The vector potential of Haemaphysalis longicornis ticks for Babesia microti parasites under experimental condition (2015)
  • Author(s): Kusakisako, K., Maeda, H., Galay, R. L., Matsuo, T., Tsujio, M., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
  • Journal Title: J. Protozool. Res. Volume: In press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evaluation and comparison of the potential of two ferritins as anti-tick vaccines against Haemaphysalis longicornis (2014)
  • Author(s): Galay, R. L., Miyata, T., Umemiya-Shirafuji, R., Maeda, H., Kusakisako, K., Tsuji, N., Mochizuki, M., Fujisaki, K. and Tanaka, T.
  • Journal Title: Parasit. Vectors Volume: 7（１） Pages: 482-482
  • Peer Reviewed / Open Access
• [Journal Article] Iron metabolism in hard ticks (Acari: Ixodidae): The antidote to their toxic diet (2014)
  • Author(s): Galay, R. L., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
  • Journal Title: Parasitol. Int. Volume: 64（2) Issue: 2 Pages: 182-189
  • DOI: 10.1016/j.parint.2014.12.005
  • Peer Reviewed
• [Journal Article] Host-derived transferrin is maintained and transferred from midgut to ovary in Haemaphysalis longicornis ticks (2014)
  • Author(s): Mori, H., Galay, R. L., Maeda, H., Matsuo, T., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K., Tanaka, T.
  • Journal Title: Ticks Tick Borne Dis. Volume: 5 (2) Issue: 2 Pages: 121-126
  • DOI: 10.1016/j.ttbdis.2013.09.004
  • Peer Reviewed
• [Journal Article] Host specificity and in vivo infectivities of the mouse coccidian parasites Eimeria krijgsmanni (2014)
  • Author(s): Hashimoto, K., Tanaka, T., Matsubayashi, M., Endo, K., Umemiya-Shirafuji, R., Matsui, T., Matsuo, T.
  • Journal Title: Acta Parasitol. Volume: 59 (2) Pages: 337-342
  • Peer Reviewed
• [Journal Article] Expression analysis of autophagy-related genes in the hard tick Haemaphysalis longicornis. (2014)
  • Author(s): Umemiya-Shirafuji R, Galay RL, Maeda H, Kawano S, Tanaka T, Fukumoto S, Suzuki H, Tsuji N, Fujisaki K.
  • Journal Title: Vet. Parasitol. Volume: 201 Issue: 1-2 Pages: 169-175
  • DOI: 10.1016/j.vetpar.2014.01.024
  • Peer Reviewed
• [Journal Article] Two kinds of ferritin protect ixodid ticks from iron overload and consequent oxidative stress. (2014)
  • Author(s): Galay RL, Umemiya-Shirafuji R, Bacolod ET, Maeda H, Kusakisako K, Koyama J, Tsuji N, Mochizuki M, Fujisaki K, Tanaka T.
  • Journal Title: PLoS One. Volume: 9 Issue: 3 Pages: e90661-e90661
  • DOI: 10.1371/journal.pone.0090661
  • Peer Reviewed / Open Access
• [Journal Article] Molecular and phylogenetic characterizations of an Eimeria krijgsmanni Yakimoff & Gouseff, 1938 (Apicomplexa: Eimeriidae) mouse intestinal protozoan parasite by partial 18S ribosomal RNA gene sequence analysis (2014)
  • Author(s): Takeo, T., Tanaka, T., Matsubayashi, M., Maeda, H., Kusakisako, K., Matsui, T., Mochizuki, M., Matsuo, T.
  • Journal Title: Parasit. Int. Volume: In press
  • Peer Reviewed
• [Presentation] 汎用的な細胞凍結保存液を用いたBabesia gibsoni原虫凍結保存法の検討およびその改良 (2016)
  • Author(s): 草木迫浩大, 正谷達謄, 屋田 友里花, Talactac Melbourne Rio, Hernandez Emmanuel Pacia, 前田大輝, 望月雅美, 田仲哲也
  • Organizer: 第85回日本寄生虫学会
  • Place of Presentation: 宮崎市民プラザ(宮崎市、宮崎)
  • Year and Date: 2016-03-19
• [Presentation] 鹿児島県各地で採取されたマダニにおけるTheileria, Babesia及びHepatozoonの分子疫学 (2016)
  • Author(s): 正谷達謄, 林 慶, 立野守洋, 遠藤泰之, 松尾智英, 田仲哲也, 御供田睦代, 関 まどか, 安藤匡子
  • Organizer: 第85回日本寄生虫学会
  • Place of Presentation: 宮崎市民プラザ(宮崎市、宮崎)
  • Year and Date: 2016-03-19
• [Presentation] フタトゲチマダニ体内におけるBabesia ovata移行動態について (2016)
  • Author(s): 前田大輝, 八田岳士, 坪川大悟, 三上房子, 松林 誠, 三好猛晴, 白藤(梅宮)梨可, 大久保和洋, 五十嵐郁男, 望月雅美, 藤崎幸蔵, 辻 尚利, 田仲哲也
  • Organizer: 第85回日本寄生虫学会
  • Place of Presentation: 宮崎市民プラザ(宮崎市、宮崎)
  • Year and Date: 2016-03-19
• [Presentation] 過酸化水素消去酵素2-Cysペルオキシレドキシンはマダニの吸血および産卵時の抗酸化機構に関与する (2015)
  • Author(s): 田仲哲也, 草木迫浩大, 正谷達謄, 宮田 健, Remil Linggatong Galay, 白藤(梅宮)梨可, 前田大輝, Melbourne Rio Talactac, 辻 尚利, 望月雅美, 藤崎幸蔵
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(神戸市、兵庫)
  • Year and Date: 2015-12-01
• [Presentation] Survival dynamics of tick-borne encephalitis virus surrogate Langat virus Haemaphysalis longicornis (2015)
  • Author(s): Melbourne Rio Talactac, Kentaro Yoshii, Tetsuya Tanaka, Kozo Fujisaki, Masami Mochizuk
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場(福岡市、福岡)
  • Year and Date: 2015-11-22
• [Presentation] Experimental evaluation of Haemaphysalis longicornis tick as a potential biologic vector of Japanese encephalitis virus (2015)
  • Author(s): Manami Goto, Hiroshi Shimoda, Ken Maeda, Hiroki Maeda, Takeshi Hatta, Naotoshi Tsuji, Tetsuya Tanaka, Masami Mochizuki
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場(福岡市、福岡)
  • Year and Date: 2015-11-22
• [Presentation] 大分県久住高原の通年放牧牛におけるTheileria orientalisジェノタイプの年間変動 (2015)
  • Author(s): 正谷達謄, 吉原俊平, 松原敦子, 後藤貴文, 高橋秀之, 田仲哲也, 安藤匡子, 遠藤泰之, 松尾智英
  • Organizer: 第68回日本寄生虫学会南日本支部大会・第65回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 長崎大学(長崎市、長崎)
  • Year and Date: 2015-10-17
• [Presentation] Identification and Expression of two Glutathione S-Transferases from the hard tick Haemaphysalis longicornis (2015)
  • Author(s): Emmanuel Pacia Hernandez, Kodai Kusakisako, Hiroki Maeda, Remil Linggatong Galay, Masami Mochizuki, Kozo Fujizaki, Tetsuya Tanaka
  • Organizer: 第68回日本寄生虫学会南日本支部大会・第65回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 長崎大学(長崎市、長崎)
  • Year and Date: 2015-10-17
• [Presentation] 2-Cys Peroxiredoxin is needed in blood feeding and next-generation production of ticks (2015)
  • Author(s): Kodai Kusakisako, Tatsunori Masatani, Takeshi Miyata, Remil Linggatong Galay, Rika Umemiya-Shirafuji, Hiroki Maeda, Melbourne Rio Talactac, Naotoshi Tsuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
  • Organizer: The 3rd Sapporo Summer Seminar for One Health
  • Place of Presentation: 北海道大学(札幌市、北海道)
  • Year and Date: 2015-09-16
  • Int'l Joint Research
• [Presentation] 病原微生物感染に関与するマダニ生物活性分子について (2015)
  • Author(s): 田仲哲也
  • Organizer: 日本衛生動物学会・日本ダニ学会共催 公開シンポジウム
  • Place of Presentation: 法政大学(千代田区、東京都)
  • Year and Date: 2015-09-11
  • Invited
• [Presentation] ベクターマダニ体内におけるBabesia ovata増殖動態についての一考察 (2015)
  • Author(s): 前田大輝, 八田岳士, 松林 誠, 三好猛晴, 白藤(梅宮)梨可, 大久保和洋, 五十嵐郁男, 辻 尚利, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学(十和田市、青森)
  • Year and Date: 2015-09-07
• [Presentation] Toxoplasma gondii K-3株のシスト形成能に関する研究 (2015)
  • Author(s): 小山田昇平, 正谷達謄, 辻尾祐志, 田仲哲也, 松井利博, 松尾智英
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学(十和田市、青森)
  • Year and Date: 2015-09-07
• [Presentation] Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus (2015)
  • Author(s): Melbourne Rio Talactac, 好井健太朗, 辻 尚利, 藤崎幸蔵, 田仲哲也, 望月雅美
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学(十和田市、青森)
  • Year and Date: 2015-09-07
• [Presentation] フタトゲチマダニにおけるペルオキシレドキシンの役割について (2015)
  • Author(s): 草木迫浩大, Remil Linggatong Galay, 白藤(梅宮)梨可, 前田大輝, Melbourne Rio Talactac, 辻 尚利, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学(八戸市、青森)
  • Year and Date: 2015-09-07
• [Presentation] Knockdown of ferritin genes increases the susceptibility of the hard tick Haemaphysalis longicornis to bacterial infection (2015)
  • Author(s): Tetsuya Tanaka, Remil Linggatong Galay, Rie Takechi, Rika Umemiya-Shirafuji, Melbourne Rio Talactac, Hiroki Maeda, Kodai Kusakisako, Masami Mochizuki, Kozo Fujisaki
  • Organizer: World Association for the Advancement of Veterinary Parasitology Congress 2015
  • Place of Presentation: The Arena and Convention Centre(Liverpool, UK)
  • Year and Date: 2015-08-16
  • Int'l Joint Research
• [Presentation] マダニ吸血におけるペルオキシレドキシンの役割について (2015)
  • Author(s): 草木迫浩大, Remil Linggatong Galay, 白藤(梅宮)梨可, 前田大輝, Melbourne Rio Talactac, 辻 尚利, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第23回SADI
  • Place of Presentation: 名取市文化会館(名取市、宮城)
  • Year and Date: 2015-06-26
• [Presentation] Decreased resistance of Haemaphysalis longicornis ticks to bacterialchallenge after knockdown of ferritin molecules (2015)
  • Author(s): Remil Linggatong Galay, 武智理恵, 白藤(梅宮)梨可, Melbourne Rio Talactac, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第23回SADI
  • Place of Presentation: 名取市文化会館(名取市、宮城)
  • Year and Date: 2015-06-26
• [Presentation] フタトゲチマダニ由来ロイシンリッチリピート保有タンパク質(HlLRR)の発現動態とバベシア原虫に及ぼす影響 (2015)
  • Author(s): 田仲哲也, 栗巣孔士, 前田大輝, 宮田 健, 草木迫浩大, Remil Linggatong Galay, Melbourne Rio Talactac, 望月雅美, 藤崎幸蔵
  • Organizer: 第23回SADI
  • Place of Presentation: 名取市文化会館(名取市、宮城)
  • Year and Date: 2015-06-26
• [Presentation] フタトゲチマダニ由来2-Cys型ペルオキシレドキシン (HlPrx2) の抗酸化部位の検索 (2015)
  • Author(s): 草木迫浩大, 正谷達謄, 宮田 健, Remil Linggatong Galay, 前田大輝, 辻 尚利, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 杏林大学(三鷹市、東京都)
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] The role of ferritin in immunity of the ixodid tick Haemaphysalis longicornis injected with Escherichia coli (2015)
  • Author(s): Remil Linggatong Galay, Rie Takechi, Rika Umemiya-Shirafuji,Melbourne Rio Talactac, Hiroki Maeda, Kodai Kusakisako, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 杏林大学(三鷹市、東京都)
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Identification of the antioxidant active sites of Haemaphysalis longicornis 2-Cys type peroxiredoxin (HlPrx2) (2015)
  • Author(s): Kodai Kusakisako, Tatsunori Masatani, Takeshi Miyata, Remil Linggatong Galay, Hiroki Maeda, Naotoshi Tsuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
  • Organizer: Joint Symposium of the 6th Veterinary Research and the 3rd Veterinary Education among Universities of Veterinary Medicine in East Asia
  • Place of Presentation: 台湾大学(台北、台湾)
  • Year and Date: 2015-02-02 – 2015-02-03
• [Presentation] ラクトフェリンによる抗マラリア原虫機能の解析 (2014)
  • Author(s): 小森園 亮, 柿本麻美子, 田仲哲也, 李 長春, 渡部久実, Ibrahim Hisham, 若林裕之,山内恒治, 宮田 健
  • Organizer: 第19回日本フードファクター学会
  • Place of Presentation: 鹿児島大学(鹿児島市、鹿児島県)
  • Year and Date: 2014-11-08 – 2014-11-09
• [Presentation] Identification and characterization of a C-type lectin like protein from the hard tick Haemaphysalis longicornis (2014)
  • Author(s): Hiroki Maeda, Takeshi Miyata, Kodai Kusakisako, Remil Linggatong Galay, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
  • Organizer: The 2nd Sapporo Summer Seminar for One Health
  • Place of Presentation: 北海道大学(札幌市、北海道)
  • Year and Date: 2014-09-24 – 2014-09-25
• [Presentation] The critical functions of ferritins in Haemaphysalis longicornis and their potential as target molecules for tick control (2014)
  • Author(s): Galay Remil Linggatong, 白藤(梅宮)梨可, 宮田 健, 草木迫浩大, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学(札幌市、北海道)
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] マウス寄生性コクシジウムEimeria krijgsmanniの生活環に関する形態学的観察 (2014)
  • Author(s): 小野結菜, 松林 誠, 川口博明, 辻尾祐志, 田仲哲也, 三好宣彰, 松井利博, 松尾智英
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学(札幌市、北海道)
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] フタトゲチマダニ由来2-Cys型ペルオキシレドキシンの機能解析 (2014)
  • Author(s): 草木迫浩大, 正谷達謄, Galay Remil Linggatong, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学(札幌市、北海道)
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] フタトゲチマダニ由来TNF Receptor Associated Factor(TRAF)の同定とその役割について (2014)
  • Author(s): 武智理恵, Galay Remil Linggatong, 前田大輝, 草木迫浩大, 松尾智英, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学(札幌市、北海道)
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] フタトゲチマダニ由来Ｃ型レクチン様蛋白質の動態および機能解析 (2014)
  • Author(s): 前田大輝, 宮田健, 草木迫浩大, Remil Linggatong Galay, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第22回分子寄生虫学ワークショップ第12回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 帯広畜産大学(帯広市、北海道)
  • Year and Date: 2014-08-31 – 2014-09-03
• [Presentation] フタトゲチマダニ由来 2-Cys 型ペルオキシレドキシンの動態 (2014)
  • Author(s): 草木迫浩大, 正谷達謄, Remil Linggatong Galay, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第22回分子寄生虫学ワークショップ第12回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 帯広畜産大学(帯広市、北海道)
  • Year and Date: 2014-08-31 – 2014-09-03
• [Presentation] The potential of recombinant ferritin 1 and ferritin 2 as anti-tick vaccine against Haemaphysalis longicornis (2014)
  • Author(s): Remil Linggatong Galay, Takeshi Miyata, Rika Umemiya-Shirafuji, Hiroki Maeda, Kodai Kusakisako, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
  • Organizer: TTP8: Ticks and Tick-Borne Pathogens
  • Place of Presentation: Cape Sun hotel(Cape Town, South Africa)
  • Year and Date: 2014-08-24 – 2014-08-29
• [Presentation] The iron-storage and antioxidant functions of two types of ferritin are critical to successful blood feeding and reproduction of the ixodid tick Haemaphysalis longicornis (2014)
  • Author(s): Galay Remil Linggatong, Umemiya-Shirafuji Rika, Maeda Hiroki, Kusakisako Kodai, Mochizuki Masami, Fujisaki Kozo, Tanaka Tetsuya
  • Organizer: The XIIIth International Congress of Parasitoplogy
  • Place of Presentation: Camino Real Hotel(Mexico City, Mexico)
  • Year and Date: 2014-08-10 – 2014-08-15
• [Presentation] Ticks survive at a threshold between engorgement and starvation (2014)
  • Author(s): Kozo Fujisaki, Naotoshi Tsuji, Xuenan Xuan, Rika Shirafuji-Umemiya, Damdinsuren Boldbaatar, Tetsuya Tanaka, Takeshi Hatta, Banzragch Battur, Badgar Battsetseg
  • Organizer: 14th International Congress of Acarology
  • Place of Presentation: 京都テルサ(京都市、京都府)
  • Year and Date: 2014-07-14 – 2014-07-18
  • Invited
• [Presentation] Identification of a C-type lectin like protein from the hard tick Haemaphysalis longicornis (2014)
  • Author(s): Hiroki Maeda, Kodai Kusakisako, Remil Linggatong Galay, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanak
  • Organizer: Joint workshop at Schofield Hall
  • Place of Presentation: ソウル大学(韓国)
• [Presentation] フタトゲチマダニ脂肪体の卵黄タンパク質前駆体合成におけるtarget of rapamycin(TOR)の役割 (2014)
  • Author(s): 白藤(梅宮)梨可, Boldbaatar Damdinsuren, Liao Min, Battur Banzragch,Rahman Md Morshedur, Kuboki Thasaneeya, Galay Remil Linggatong, 田仲哲也, 藤崎幸蔵
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学(愛媛)
• [Presentation] 組換えフェリチンのフタトゲチマダニに対する抗マダニワクチンとしての可能性 (2014)
  • Author(s): ガライ レミル リングガトン, 宮田 健, 白藤(梅宮)梨可, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学(愛媛)
• [Presentation] Expression profiles of autophagy-related genes in the hard tick, Haemaphysalis longicornis (2013)
  • Author(s): Rika Umemiya-Shirafuji, Suguru Kawano, Remil Linggatong Galay, Hiroki Maeda, Aiko Kume, Tetsuya Tanaka, Hiroshi Suzuki, Kozo Fujisaki
  • Organizer: 24th International Conference of the World Association for the Advancement of Veterinary Parasitology
  • Place of Presentation: Perth Convention Exhibition Centre Western Australia(Australia)
• [Presentation] Ferritins protect the hard tick Haemaphysalis longicornis from iron-mediated oxidative stress (2013)
  • Author(s): Remil Linggatong Galay, 草木迫浩大, 前田大輝, 白藤(梅宮)梨可, Eugene Tan Bacolod, 小山次郎, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学(岐阜市)
• [Presentation] フタトゲチマダニ由来C型レクチン様蛋白質の同定とその特性解明 (2013)
  • Author(s): 前田大輝, 草木迫浩大, Remil Linggatong Galay, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学(岐阜市)
• [Presentation] フタトゲチマダニ体内におけるB. microtiの検出 (2013)
  • Author(s): 草木迫浩大, 前田大輝, Remil Linggatong Galay, 白藤(梅宮)梨可, 松尾智英, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学(岐阜市)
• [Presentation] 犬と猫のウイルス病におけるダニ媒介性伝播の実験的考察 (2013)
  • Author(s): 森 寛行, 田仲哲也, 望月雅美
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学(岐阜市)
• [Presentation] マウス腸管寄生性コクシジウムEimeria krijgsmanniの薬剤感受性 (2013)
  • Author(s): 竹尾俊規, 田仲哲也, 松林 誠, 辻尾祐志, 橋本和樹, 辻 尚利, 藤崎幸蔵, 松井利博, 松尾智英
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学(岐阜市)
• [Presentation] マウス寄生性コクシジウムEimeria krijgsmanniの宿主特異性と感染性に関する研究 (2013)
  • Author(s): 橋本和樹, 田仲哲也, 松林 誠, 遠藤恭子, 白藤(梅宮)梨可, 松井利博, 松尾智英
  • Organizer: 第66回日本寄生虫学会南日本支部大会・第63回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 大分大学(大分市)
• [Presentation] フタトゲチマダニ由来ロイシンリッチリピートドメイン保有蛋白質の動態および機能解析 (2013)
  • Author(s): 栗巣孔士, 前田大輝, Remil Linggatong Galay, 武智理恵, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第66回日本寄生虫学会南日本支部大会・第63回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 大分大学(大分市)
• [Presentation] フタトゲチマダニ由来TNF Receptor Associated Factorの同定とその役割について (2013)
  • Author(s): 武智理恵, Remil Linggatong Galay, 前田大輝, 栗巣孔士, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第66回日本寄生虫学会南日本支部大会・第63回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 大分大学(大分市)
• [Presentation] フタトゲチマダニの鉄依存性酸化ストレスから保護するフェリチンの重要性 (2013)
  • Author(s): 田仲哲也, Remil Linggatong Galay, 白藤(梅宮)梨可, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
• [Book] Vaccine Design: Methods and Protocols (2016)
  • Author(s): Galay, R. L., Miyata, T., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
  • Total Pages: 848
  • Publisher: Springer New York
• [Book] RNA Interference (2016)
  • Author(s): Galay, R. L., Miyata, T., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
  • Total Pages: 460
  • Publisher: InTech-Open Access Publisher
• [Book] 第2章 免疫の概念（獣医学教育モデル・コア・カリキュラム準拠 獣医免疫学） (2015)
  • Author(s): 田仲哲也、池田輝雄、岡本まり子、松本安喜（編集：池田輝雄、小川健司、松本安喜）
  • Total Pages: 17
  • Publisher: （株）緑書房
• [Book] 第6章 節足動物各論I（ダニ）（獣医学教育モデル・コア・カリキュラム準拠 寄生虫病学） (2014)
  • Author(s): 田仲哲也 （編集：堀井洋一郎、野中成晃、加藤大智、平 恵介、松本 淳、横山直明）
  • Total Pages: 5
  • Publisher: （株）緑書房
• [Remarks] 鹿児島大学 共同獣医学部獣医学科 病態予防獣医学講座 感染症学分野
  • URL: http://www.vet.kagoshima-u.ac.jp/kadai/V-Infection/sinkoukansen/index.html
• [Remarks] 鹿児島大学 共同獣医学部獣医学科 病態予防獣医学講座 感染症学分野
  • URL: http://www.vet.kagoshima-u.ac.jp/kadai/V-infection/sinkoukansen/top.html
• [Remarks] 鹿児島大学 共同獣医学部獣医学科 病態予防獣医学講座 感染症学分野
  • URL: http://www.vet.kagoshima-u.ac.jp/kadai/V-infection/sinkoukansen/top.html
• [Patent(Industrial Property Rights)] 抗微生物ポリペプチド (2016)
  • Inventor(s): 田仲哲也、メルボルン リオ タラクタック、藤崎幸蔵
  • Industrial Property Rights Holder: 鹿児島大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-07-15
• [Patent(Industrial Property Rights)] 抗マダニワクチン (2014)
  • Inventor(s): 田仲哲也, ガライ レミル リンガートン, 藤崎幸蔵
  • Industrial Property Rights Holder: 田仲哲也, ガライ レミル リンガートン, 藤崎幸蔵
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-02-28