| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Outline of Final Research Achievements |
Toll like receptor (TLR) and tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) are very important to protect againt pathogen infection. Therefore, we alalyzed to idetify and characterize TLR and TRAFD1 from the hard tick Haemaphysalis longicornis. Knockdown of TRAFD1 gene by RNA interference did not affect blood feeding or oviposition. However, TRAFD1 silencing affected the expression of the longicin gene, a defensin-like molecule. On the other hand,recombinant TLR demonstrated growth inhibition activity against B. gibsoni in vitro without a hemolytic effect at used concentration .These results suggested that TLR and TRAFD1 strongly affected innate immunity of ticks.
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