New function of interleukin-19 in type-IV contact hypersensitivity

• Project/Area Number: 25292174
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Osaka Prefecture University
• Principal Investigator: Azuma Yasu-Taka 大阪府立大学, 生命環境科学研究科, 准教授 (50298816)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 皮膚炎 / Th17応答 / IL-19 / T細胞 / 炎症 / CHS / アレルギー / サイトカイン / DTH

Outline of Final Research Achievements

IL-19 is a member of the IL-10 family of interleukins and is an immuno-modulatory cytokine produced by the main macrophages. There is an increasing focus on the interaction and relationship of IL-19 with the function of T cells. Contact hypersensitivity (CHS) is T cell-mediated cutaneous inflammation. Therefore, we asked whether IL-19 causes CHS. We investigated the immunological role of IL-19 in CHS induced by 1-fluoro-2,4-dinitrofluorobenzene as a hapten. IL-19 was highly expressed in skin exposed to the hapten, and ear swelling was increased in IL-19 knockout mice. The exacerbation of the CHS response in IL-19 knockout mice correlated with increased levels of IL-17 and IL-6, but no alterations were noted in the production of IFNgamma and IL-4 in the T cells of the lymph nodes. In addition to the effect on T cell response, IL-19 knockout mice increased production of inflammatory cytokines. These results show that IL-19 suppressed hapten-dependent skin inflammation in the CHS.

Research Products

• [Journal Article] The Role of Interleukin-19 in Contact Hypersensitivity (2018)
  • Author(s): Fujimoto Y, Fujita T, Kuramoto N, Kuwamura M, Izawa T, Nishiyama K, Yoshida N, Nakajima H, Takeuchi T, Azuma YT.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 41 Issue: 2 Pages: 182-189
  • DOI: 10.1248/bpb.b17-00594
  • NAID: 130006329250
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Interleukin-19 contributes to T cell-mediated contact hypersensitivity (2018)
  • Author(s): Yasuyuki Fujimoto, Hidemitsu Nakajima, Tadayoshi Takeuchi, and Yasu-Taka Azuma
  • Organizer: 18th World Congress of Basic and Clinical Pharmacology (WCP2018)
  • Int'l Joint Research
• [Presentation] アレルギー性皮膚炎におけるIL-19を介した新規調節機構の解明． (2017)
  • Author(s): 藤本泰之、東 泰孝、中嶋秀満、竹内正吉
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] 接触型過敏症におけるIL-19の機能的役割． (2015)
  • Author(s): 藤本泰之，東 泰孝，中嶋秀満，竹内正吉
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学（十和田市、青森県）
  • Year and Date: 2015-09-08
• [Presentation] Role of IL-19 in T cell-mediated immune responses. (2014)
  • Author(s): 東 泰孝、松尾有希子、中嶋秀満、竹内正吉
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 仙台市